Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response

Objective To investigate the effects of apoptotic bodies (ABs) derived from dental pulp stem cells (DPSCs) on macrophage polarization and inflammation response in vivo. Methods Human DPSCs were extracted, cultured and identified. Staurosporine was used to apoptosis induction and differential methods...

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Autores principales:	GONG Shengkai, YANG Xiaoshan, DOU Geng, LI Zihan, LIU Siying, WANG Wei, LIU Shiyu
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2022
Materias:	dental pulp stem cells macrophages macrophages polarization inflammatory response apoptosis apoptotic bodies tissue regeneration stem cell transplantation inflammatory regulation Medicine R
Acceso en línea:	https://doaj.org/article/aa6876596ecb459d8515060feb1ddfb8
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spelling	oai:doaj.org-article:aa6876596ecb459d8515060feb1ddfb82021-11-23T03:44:36ZDental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response10.12016/j.issn.2096-1456.2022.01.0032096-1456https://doaj.org/article/aa6876596ecb459d8515060feb1ddfb82022-01-01T00:00:00Zhttp://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2022.01.003https://doaj.org/toc/2096-1456Objective To investigate the effects of apoptotic bodies (ABs) derived from dental pulp stem cells (DPSCs) on macrophage polarization and inflammation response in vivo. Methods Human DPSCs were extracted, cultured and identified. Staurosporine was used to apoptosis induction and differential methods were performed for ABs identification. The in vitro cultured macrophages were divided into 3 groups: solvent control, lipopolysaccharide (LPS), and the LPS+ABs. The macrophages were stimulated with LPS to induce inflammation followed by ABs treatment. In the untreated group, macrophages were added with an equal amount of solvent. The specific uptake of ABs by macrophages, the expression level of CD206 and the levels of inflammatory cytokines were analyzed. The mouse models of cutaneous wounds and dextran sulfate sodium (DSS)-induced colitis were established, and the mice were randomly divided into 3 groups: the PBS-treated group, the DPSCs-treated group, and the ABs-treated group. The mice were injected with the same volume of PBS, DPSCs and ABs, respectively. The body weight, histological pathology, the expression levels of CD206 and cytokines, and the extent of tissue regeneration were measured. Results DPSCs and ABs derived from DPSCs were successfully isolated and characterized. ABs could be taken up by macrophage. While lipopolysaccharide(LPS) induced production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), ABs significantly reduced the levels of these pro-inflammatory cytokines and increased the expression of transforming growth factor-β (TGF-β) and CD206 (P < 0.01). In the cutaneous inflammatory wound model, the wound closure rate in mice intravenously injected with ABs was significantly accelerated (P < 0.05). The administration of ABs markedly reduced the pro-inflammatory factors levels and increased the CD206+ cell number. In the colitis model, treatment with ABs markedly reduced the loss in bodyweight (P < 0.05), recovered the colon length (P < 0.01), and significantly increased the CD206+ cell number. Conclusion DPSCs-derived ABs could enhance macrophage M2 polarization and attenuate inflammation. Therefore, ABs could be used as a promising cell replacement for inflammatory regulation and tissue regeneration.GONG ShengkaiYANG XiaoshanDOU GengLI ZihanLIU SiyingWANG WeiLIU ShiyuEditorial Department of Journal of Prevention and Treatment for Stomatological Diseasesarticledental pulp stem cellsmacrophagesmacrophages polarizationinflammatory responseapoptosisapoptotic bodiestissue regenerationstem cell transplantationinflammatory regulationMedicineRZH口腔疾病防治, Vol 30, Iss 1, Pp 12-19 (2022)
institution	DOAJ
collection	DOAJ
language	ZH
topic	dental pulp stem cells macrophages macrophages polarization inflammatory response apoptosis apoptotic bodies tissue regeneration stem cell transplantation inflammatory regulation Medicine R
spellingShingle	dental pulp stem cells macrophages macrophages polarization inflammatory response apoptosis apoptotic bodies tissue regeneration stem cell transplantation inflammatory regulation Medicine R GONG Shengkai YANG Xiaoshan DOU Geng LI Zihan LIU Siying WANG Wei LIU Shiyu Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
description	Objective To investigate the effects of apoptotic bodies (ABs) derived from dental pulp stem cells (DPSCs) on macrophage polarization and inflammation response in vivo. Methods Human DPSCs were extracted, cultured and identified. Staurosporine was used to apoptosis induction and differential methods were performed for ABs identification. The in vitro cultured macrophages were divided into 3 groups: solvent control, lipopolysaccharide (LPS), and the LPS+ABs. The macrophages were stimulated with LPS to induce inflammation followed by ABs treatment. In the untreated group, macrophages were added with an equal amount of solvent. The specific uptake of ABs by macrophages, the expression level of CD206 and the levels of inflammatory cytokines were analyzed. The mouse models of cutaneous wounds and dextran sulfate sodium (DSS)-induced colitis were established, and the mice were randomly divided into 3 groups: the PBS-treated group, the DPSCs-treated group, and the ABs-treated group. The mice were injected with the same volume of PBS, DPSCs and ABs, respectively. The body weight, histological pathology, the expression levels of CD206 and cytokines, and the extent of tissue regeneration were measured. Results DPSCs and ABs derived from DPSCs were successfully isolated and characterized. ABs could be taken up by macrophage. While lipopolysaccharide(LPS) induced production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), ABs significantly reduced the levels of these pro-inflammatory cytokines and increased the expression of transforming growth factor-β (TGF-β) and CD206 (P < 0.01). In the cutaneous inflammatory wound model, the wound closure rate in mice intravenously injected with ABs was significantly accelerated (P < 0.05). The administration of ABs markedly reduced the pro-inflammatory factors levels and increased the CD206+ cell number. In the colitis model, treatment with ABs markedly reduced the loss in bodyweight (P < 0.05), recovered the colon length (P < 0.01), and significantly increased the CD206+ cell number. Conclusion DPSCs-derived ABs could enhance macrophage M2 polarization and attenuate inflammation. Therefore, ABs could be used as a promising cell replacement for inflammatory regulation and tissue regeneration.
format	article
author	GONG Shengkai YANG Xiaoshan DOU Geng LI Zihan LIU Siying WANG Wei LIU Shiyu
author_facet	GONG Shengkai YANG Xiaoshan DOU Geng LI Zihan LIU Siying WANG Wei LIU Shiyu
author_sort	GONG Shengkai
title	Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
title_short	Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
title_full	Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
title_fullStr	Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
title_full_unstemmed	Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
title_sort	dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response
publisher	Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
publishDate	2022
url	https://doaj.org/article/aa6876596ecb459d8515060feb1ddfb8
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_version_	1718417339587756032

Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response

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