TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.

Local cultivars of A. caudatus: Helios and Karmin were used as plant material. Amaranth is a new pseudocereal introduced in Ukraine. The plant biomass of amaranth is used in medicine, food industry and cosmetology industry. Aim. The purpose of the work was to identify the optimal conditions for t...

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Autores principales:	O. M. Yaroshko, M. V. Kuchuk
Formato:	article
Lenguaje:	EN RU UK
Publicado:	National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2021
Materias:	amaranthus uida gfp agrobacterium transient expression. Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/aa74be0c88a34071b1de86654fc94f87
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spelling	oai:doaj.org-article:aa74be0c88a34071b1de86654fc94f872021-11-03T16:56:11ZTRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.2410-77512410-776Xhttps://doaj.org/article/aa74be0c88a34071b1de86654fc94f872021-08-01T00:00:00Zhttp://biotechnology.kiev.ua/images/4_2021/yaroshko_4_2021.pdfhttps://doaj.org/toc/2410-7751https://doaj.org/toc/2410-776XLocal cultivars of A. caudatus: Helios and Karmin were used as plant material. Amaranth is a new pseudocereal introduced in Ukraine. The plant biomass of amaranth is used in medicine, food industry and cosmetology industry. Aim. The purpose of the work was to identify the optimal conditions for the transient expression of reporter genes in Amaranthus caudatus cultivars. Methods. Biochemical and microscopy methods were used in the following work. Seedlings and adult plants of different age were infiltrated with agrobacterial suspensions separately (genetic vector pCBV19 with a uidA gene and genetic vector pNMD2501 with a gfp gene in Agrobacterium tumefaciens GV3101 strain). Results. Transient expression of the uidA and gfp genes was obtained in amaranth plants after conduction series of experiments. The most intensive transient expression of gfp and uidA genes was observed in seedlings infiltrated at the age of 1 day. The maximum fluorescence of the GFP protein was observed on 5th–6th days. Conclusions. It was shown that the cultivar Helios was more susceptible to agrobacterial infection than the cultivar Karmin. The effectiveness of Agrobacterium mediated transformation was from 16% to 95% for the Helios cultivar and from 12% to 93% for the Karmin cultivar. The obtained results indicate that the studied amaranth cultivars can potentially be used for obtaining transient expression of target genes and synthesizing target proteins in their tissues in the future.O. M. YaroshkoM. V. KuchukNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.articleamaranthusuidagfpagrobacteriumtransient expression.BiotechnologyTP248.13-248.65ENRUUKBiotechnologia Acta, Vol 14, Iss 4, Pp 53-63 (2021)
institution	DOAJ
collection	DOAJ
language	EN RU UK
topic	amaranthus uida gfp agrobacterium transient expression. Biotechnology TP248.13-248.65
spellingShingle	amaranthus uida gfp agrobacterium transient expression. Biotechnology TP248.13-248.65 O. M. Yaroshko M. V. Kuchuk TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.
description	Local cultivars of A. caudatus: Helios and Karmin were used as plant material. Amaranth is a new pseudocereal introduced in Ukraine. The plant biomass of amaranth is used in medicine, food industry and cosmetology industry. Aim. The purpose of the work was to identify the optimal conditions for the transient expression of reporter genes in Amaranthus caudatus cultivars. Methods. Biochemical and microscopy methods were used in the following work. Seedlings and adult plants of different age were infiltrated with agrobacterial suspensions separately (genetic vector pCBV19 with a uidA gene and genetic vector pNMD2501 with a gfp gene in Agrobacterium tumefaciens GV3101 strain). Results. Transient expression of the uidA and gfp genes was obtained in amaranth plants after conduction series of experiments. The most intensive transient expression of gfp and uidA genes was observed in seedlings infiltrated at the age of 1 day. The maximum fluorescence of the GFP protein was observed on 5th–6th days. Conclusions. It was shown that the cultivar Helios was more susceptible to agrobacterial infection than the cultivar Karmin. The effectiveness of Agrobacterium mediated transformation was from 16% to 95% for the Helios cultivar and from 12% to 93% for the Karmin cultivar. The obtained results indicate that the studied amaranth cultivars can potentially be used for obtaining transient expression of target genes and synthesizing target proteins in their tissues in the future.
format	article
author	O. M. Yaroshko M. V. Kuchuk
author_facet	O. M. Yaroshko M. V. Kuchuk
author_sort	O. M. Yaroshko
title	TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.
title_short	TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.
title_full	TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.
title_fullStr	TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.
title_full_unstemmed	TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.
title_sort	transient expression of reporter genes in cultivars of amaranthus caudatus l.
publisher	National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
publishDate	2021
url	https://doaj.org/article/aa74be0c88a34071b1de86654fc94f87
work_keys_str_mv	AT omyaroshko transientexpressionofreportergenesincultivarsofamaranthuscaudatusl AT mvkuchuk transientexpressionofreportergenesincultivarsofamaranthuscaudatusl
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TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.

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