Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle

This study aims to screen potential regulators and regulate fecundity networks between microRNAs (miRNAs) and target genes. The bovine testes of immature and mature Chinese Red Steppes were performed by genome-wide analysis of mRNAs and miRNAs. Compared with testicular tissues of newborns, 6051 upre...

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Autores principales:	Xibi Fang, Lihong Qin, Haibin Yu, Ping Jiang, Lixin Xia, Zhen Gao, Runjun Yang, Yumin Zhao, Xianzhong Yu, Zhihui Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cattle male reproduction microRNA functional gene Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/aa7783bcc5c04a329c47bf2c19fa4f6f
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spelling	oai:doaj.org-article:aa7783bcc5c04a329c47bf2c19fa4f6f2021-11-25T16:13:48ZComprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle10.3390/ani111130242076-2615https://doaj.org/article/aa7783bcc5c04a329c47bf2c19fa4f6f2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3024https://doaj.org/toc/2076-2615This study aims to screen potential regulators and regulate fecundity networks between microRNAs (miRNAs) and target genes. The bovine testes of immature and mature Chinese Red Steppes were performed by genome-wide analysis of mRNAs and miRNAs. Compared with testicular tissues of newborns, 6051 upregulated genes and 7104 downregulated genes in adult cattle were identified as differentially expressed genes (DEGs). The DEGs were significantly enriched in 808 GO terms (<i>p</i> < 0.05) including male gonad development, male genitalia development, spermatogenesis, and sperm motility. Moreover, DEGs were also significantly enriched in 105 KEGG pathways (<i>p</i> < 0.05), including cGMP-PKG signaling pathway and calcium signaling pathway. To explore the expression of miRNA-regulated gene expression, 896 differentially expressed target genes negatively regulated with the expression levels of 31 differentially expressed miRNAs (DERs) were predicted and analyzed, and a network-integrated analysis was constructed. Furthermore, real-time PCR was performed to verify the expression levels of DEGs and DERs. Our results identified novel candidate DEGs and DERs correlated with male reproduction and intricate regulating networks between miRNAs and genes, which will be valuable for future genetic and epigenetic studies of sperm development and maturity, as well as providing valuable insights into the molecular mechanisms of male fertility and spermatogenesis in cattle.Xibi FangLihong QinHaibin YuPing JiangLixin XiaZhen GaoRunjun YangYumin ZhaoXianzhong YuZhihui ZhaoMDPI AGarticlecattlemale reproductionmicroRNAfunctional geneVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3024, p 3024 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cattle male reproduction microRNA functional gene Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	cattle male reproduction microRNA functional gene Veterinary medicine SF600-1100 Zoology QL1-991 Xibi Fang Lihong Qin Haibin Yu Ping Jiang Lixin Xia Zhen Gao Runjun Yang Yumin Zhao Xianzhong Yu Zhihui Zhao Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle
description	This study aims to screen potential regulators and regulate fecundity networks between microRNAs (miRNAs) and target genes. The bovine testes of immature and mature Chinese Red Steppes were performed by genome-wide analysis of mRNAs and miRNAs. Compared with testicular tissues of newborns, 6051 upregulated genes and 7104 downregulated genes in adult cattle were identified as differentially expressed genes (DEGs). The DEGs were significantly enriched in 808 GO terms (<i>p</i> < 0.05) including male gonad development, male genitalia development, spermatogenesis, and sperm motility. Moreover, DEGs were also significantly enriched in 105 KEGG pathways (<i>p</i> < 0.05), including cGMP-PKG signaling pathway and calcium signaling pathway. To explore the expression of miRNA-regulated gene expression, 896 differentially expressed target genes negatively regulated with the expression levels of 31 differentially expressed miRNAs (DERs) were predicted and analyzed, and a network-integrated analysis was constructed. Furthermore, real-time PCR was performed to verify the expression levels of DEGs and DERs. Our results identified novel candidate DEGs and DERs correlated with male reproduction and intricate regulating networks between miRNAs and genes, which will be valuable for future genetic and epigenetic studies of sperm development and maturity, as well as providing valuable insights into the molecular mechanisms of male fertility and spermatogenesis in cattle.
format	article
author	Xibi Fang Lihong Qin Haibin Yu Ping Jiang Lixin Xia Zhen Gao Runjun Yang Yumin Zhao Xianzhong Yu Zhihui Zhao
author_facet	Xibi Fang Lihong Qin Haibin Yu Ping Jiang Lixin Xia Zhen Gao Runjun Yang Yumin Zhao Xianzhong Yu Zhihui Zhao
author_sort	Xibi Fang
title	Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle
title_short	Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle
title_full	Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle
title_fullStr	Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle
title_full_unstemmed	Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle
title_sort	comprehensive analysis of mirnas and target mrnas between immature and mature testis tissue in chinese red steppes cattle
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/aa7783bcc5c04a329c47bf2c19fa4f6f
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Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle

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