Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds

Qi Tan, Hao Tang, Jianguo Hu, Yerong Hu, Xinmin Zhou, Yunming Tao, Zhongshi WuDepartment of Cardiothoracic Surgery Second Xiangya Hospital, Central South University, Changsha 410011, People&#39;s Republic of ChinaAbstract: Regeneration deficiency is one of the main obstacles limiting the eff...

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Autores principales:	Tan Q, Tang H, Hu J, Hu Y, Zhou X, Tao R, Wu Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/aa77e3074a7e4523a204c0dc24b00b15
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spelling	oai:doaj.org-article:aa77e3074a7e4523a204c0dc24b00b152021-12-02T04:54:04ZControlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds1176-91141178-2013https://doaj.org/article/aa77e3074a7e4523a204c0dc24b00b152011-05-01T00:00:00Zhttp://www.dovepress.com/controlled-release-of-chitosanheparin-nanoparticle-delivered-vegf-enha-a7323https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Qi Tan, Hao Tang, Jianguo Hu, Yerong Hu, Xinmin Zhou, Yunming Tao, Zhongshi WuDepartment of Cardiothoracic Surgery Second Xiangya Hospital, Central South University, Changsha 410011, People&#39;s Republic of ChinaAbstract: Regeneration deficiency is one of the main obstacles limiting the effectiveness of tissue-engineered scaffolds. To develop scaffolds that are capable of accelerating regeneration, we created a heparin/chitosan nanoparticle-immobilized decellularized bovine jugular vein scaffold to increase the loading capacity and allow for controlled release of vascular endothelial growth factor (VEGF). The vascularization of the scaffold was evaluated in vitro and in vivo. The functional nanoparticles were prepared by physical self-assembly with a diameter of 67&ndash;132 nm, positive charge, and a zeta potential of ~30 mV and then the nanoparticles were successfully immobilized to the nanofibers of scaffolds by ethylcarbodiimide hydrochloride/hydroxysulfosuccinimide modification. The scaffolds immobilized with heparin/chitosan nanoparticles exhibited highly effective localization and sustained release of VEGF for several weeks in vitro. This modified scaffold significantly stimulated endothelial cells&#39; proliferation in vitro. Importantly, utilization of heparin/chitosan nanoparticles to localize VEGF significantly increased fibroblast infiltration, extracellular matrix production, and accelerated vascularization in mouse subcutaneous implantation model in vivo. This study provided a novel and promising system for accelerated regeneration of tissue-engineering scaffolds.Keywords: nanoparticle, scaffolds, VEGF, control release, vascularization, regenerationTan QTang HHu JHu YZhou XTao RWu ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 929-942 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tan Q Tang H Hu J Hu Y Zhou X Tao R Wu Z Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds
description	Qi Tan, Hao Tang, Jianguo Hu, Yerong Hu, Xinmin Zhou, Yunming Tao, Zhongshi WuDepartment of Cardiothoracic Surgery Second Xiangya Hospital, Central South University, Changsha 410011, People&#39;s Republic of ChinaAbstract: Regeneration deficiency is one of the main obstacles limiting the effectiveness of tissue-engineered scaffolds. To develop scaffolds that are capable of accelerating regeneration, we created a heparin/chitosan nanoparticle-immobilized decellularized bovine jugular vein scaffold to increase the loading capacity and allow for controlled release of vascular endothelial growth factor (VEGF). The vascularization of the scaffold was evaluated in vitro and in vivo. The functional nanoparticles were prepared by physical self-assembly with a diameter of 67&ndash;132 nm, positive charge, and a zeta potential of ~30 mV and then the nanoparticles were successfully immobilized to the nanofibers of scaffolds by ethylcarbodiimide hydrochloride/hydroxysulfosuccinimide modification. The scaffolds immobilized with heparin/chitosan nanoparticles exhibited highly effective localization and sustained release of VEGF for several weeks in vitro. This modified scaffold significantly stimulated endothelial cells&#39; proliferation in vitro. Importantly, utilization of heparin/chitosan nanoparticles to localize VEGF significantly increased fibroblast infiltration, extracellular matrix production, and accelerated vascularization in mouse subcutaneous implantation model in vivo. This study provided a novel and promising system for accelerated regeneration of tissue-engineering scaffolds.Keywords: nanoparticle, scaffolds, VEGF, control release, vascularization, regeneration
format	article
author	Tan Q Tang H Hu J Hu Y Zhou X Tao R Wu Z
author_facet	Tan Q Tang H Hu J Hu Y Zhou X Tao R Wu Z
author_sort	Tan Q
title	Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds
title_short	Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds
title_full	Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds
title_fullStr	Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds
title_full_unstemmed	Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds
title_sort	controlled release of chitosan/heparin nanoparticle-delivered vegf enhances regeneration of decellularized tissue-engineered scaffolds
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/aa77e3074a7e4523a204c0dc24b00b15
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Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds

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