Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus

Abstract The adaptive ability of sperm in the female reproductive tract micromilieu signifies the successful fertilization process. The study aimed to analyze the preparedness of sperm to the prevailing osmotic and pH stressors in the female reproductive tract. Fresh bovine sperm were incubated in 2...

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Autores principales:	Maharajan Lavanya, Santhanahalli Siddalingappa Archana, Divakar Swathi, Laxman Ramya, Arunachalam Arangasamy, Balakrishnan Binsila, Arindam Dhali, Narayanan Krishnaswamy, Sanjay Kumar Singh, Harendra Kumar, Muniandy Sivaram, Sellappan Selvaraju
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa77fb96b41c49e4aa92d9ff6553991e
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spelling	oai:doaj.org-article:aa77fb96b41c49e4aa92d9ff6553991e2021-11-21T12:23:50ZSperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus10.1038/s41598-021-01928-62045-2322https://doaj.org/article/aa77fb96b41c49e4aa92d9ff6553991e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01928-6https://doaj.org/toc/2045-2322Abstract The adaptive ability of sperm in the female reproductive tract micromilieu signifies the successful fertilization process. The study aimed to analyze the preparedness of sperm to the prevailing osmotic and pH stressors in the female reproductive tract. Fresh bovine sperm were incubated in 290 (isosmotic-control), 355 (hyperosmotic-uterus and oviduct), and 420 (hyperosmotic-control) mOsm/kg and each with pH of 6.8 (uterus) and 7.4 (oviduct). During incubation, the changes in sperm functional attributes were studied. Sperm kinematics and head area decreased significantly (p < 0.05) immediately upon exposure to hyperosmotic stress at both pH. Proportion of sperm capacitated (%) in 355 mOsm/kg at 1 and 2 h of incubation were significantly (p < 0.05) higher than those in 290 mOsm media. The magnitude and duration of recovery of sperm progressive motility in 355 mOsm with pH 7.4 was correlated with the ejaculate rejection rate (R2 = 0.7). Using this information, the bulls were divided into good (n = 5) and poor (n = 5) osmo-adapters. The osmo-responsive genes such as NFAT5, HSP90AB1, SLC9C1, ADAM1B and GAPDH were upregulated (p < 0.05) in the sperm of good osmo-adapters. The study suggests that sperm are prepared for the osmotic and pH challenges in the female reproductive tract and the osmoadaptive ability is associated with ejaculate quality in bulls.Maharajan LavanyaSanthanahalli Siddalingappa ArchanaDivakar SwathiLaxman RamyaArunachalam ArangasamyBalakrishnan BinsilaArindam DhaliNarayanan KrishnaswamySanjay Kumar SinghHarendra KumarMuniandy SivaramSellappan SelvarajuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maharajan Lavanya Santhanahalli Siddalingappa Archana Divakar Swathi Laxman Ramya Arunachalam Arangasamy Balakrishnan Binsila Arindam Dhali Narayanan Krishnaswamy Sanjay Kumar Singh Harendra Kumar Muniandy Sivaram Sellappan Selvaraju Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus
description	Abstract The adaptive ability of sperm in the female reproductive tract micromilieu signifies the successful fertilization process. The study aimed to analyze the preparedness of sperm to the prevailing osmotic and pH stressors in the female reproductive tract. Fresh bovine sperm were incubated in 290 (isosmotic-control), 355 (hyperosmotic-uterus and oviduct), and 420 (hyperosmotic-control) mOsm/kg and each with pH of 6.8 (uterus) and 7.4 (oviduct). During incubation, the changes in sperm functional attributes were studied. Sperm kinematics and head area decreased significantly (p < 0.05) immediately upon exposure to hyperosmotic stress at both pH. Proportion of sperm capacitated (%) in 355 mOsm/kg at 1 and 2 h of incubation were significantly (p < 0.05) higher than those in 290 mOsm media. The magnitude and duration of recovery of sperm progressive motility in 355 mOsm with pH 7.4 was correlated with the ejaculate rejection rate (R2 = 0.7). Using this information, the bulls were divided into good (n = 5) and poor (n = 5) osmo-adapters. The osmo-responsive genes such as NFAT5, HSP90AB1, SLC9C1, ADAM1B and GAPDH were upregulated (p < 0.05) in the sperm of good osmo-adapters. The study suggests that sperm are prepared for the osmotic and pH challenges in the female reproductive tract and the osmoadaptive ability is associated with ejaculate quality in bulls.
format	article
author	Maharajan Lavanya Santhanahalli Siddalingappa Archana Divakar Swathi Laxman Ramya Arunachalam Arangasamy Balakrishnan Binsila Arindam Dhali Narayanan Krishnaswamy Sanjay Kumar Singh Harendra Kumar Muniandy Sivaram Sellappan Selvaraju
author_facet	Maharajan Lavanya Santhanahalli Siddalingappa Archana Divakar Swathi Laxman Ramya Arunachalam Arangasamy Balakrishnan Binsila Arindam Dhali Narayanan Krishnaswamy Sanjay Kumar Singh Harendra Kumar Muniandy Sivaram Sellappan Selvaraju
author_sort	Maharajan Lavanya
title	Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus
title_short	Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus
title_full	Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus
title_fullStr	Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus
title_full_unstemmed	Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus
title_sort	sperm preparedness and adaptation to osmotic and ph stressors relate to functional competence of sperm in bos taurus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aa77fb96b41c49e4aa92d9ff6553991e
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Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus

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