A novel human ex vivo skin model to study early local responses to burn injuries

Abstract Burn injuries initiate numerous processes such as heat shock response, inflammation and tissue regeneration. Reliable burn models are needed to elucidate the exact sequence of local events to be able to better predict when local inflammation triggers systemic inflammatory processes. In cont...

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Autores principales:	Elisabeth Hofmann, Julia Fink, Anita Eberl, Eva-Maria Prugger, Dagmar Kolb, Hanna Luze, Simon Schwingenschuh, Thomas Birngruber, Christoph Magnes, Selma I. Mautner, Lars-Peter Kamolz, Petra Kotzbeck
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa79ae995bc14dc48e7d3647aad13c78
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spelling	oai:doaj.org-article:aa79ae995bc14dc48e7d3647aad13c782021-12-02T14:12:45ZA novel human ex vivo skin model to study early local responses to burn injuries10.1038/s41598-020-79683-32045-2322https://doaj.org/article/aa79ae995bc14dc48e7d3647aad13c782021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79683-3https://doaj.org/toc/2045-2322Abstract Burn injuries initiate numerous processes such as heat shock response, inflammation and tissue regeneration. Reliable burn models are needed to elucidate the exact sequence of local events to be able to better predict when local inflammation triggers systemic inflammatory processes. In contrast to other ex vivo skin culture approaches, we used fresh abdominal skin explants to introduce contact burn injuries. Histological and ultrastructural analyses confirmed a partial-thickness burn pathology. Gene expression patterns and cytokine production profiles of key mediators of the local inflammation, heat shock response, and tissue regeneration were analyzed for 24 h after burn injury. We found significantly increased expression of factors involved in tissue regeneration and inflammation soon after burn injury. To investigate purely inflammation-mediated reactions we injected lipopolysaccharide into the dermis. In comparison to burn injury, lipopolysaccharide injection initiated an inflammatory response while expression patterns of heat shock and tissue regeneration genes were unaffected for the duration of the experiment. This novel ex vivo human skin model is suitable to study the local, early responses to skin injuries such as burns while maintaining an intact overall tissue structure and it gives valuable insights into local mechanisms at the very beginning of the wound healing process after burn injuries.Elisabeth HofmannJulia FinkAnita EberlEva-Maria PruggerDagmar KolbHanna LuzeSimon SchwingenschuhThomas BirngruberChristoph MagnesSelma I. MautnerLars-Peter KamolzPetra KotzbeckNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisabeth Hofmann Julia Fink Anita Eberl Eva-Maria Prugger Dagmar Kolb Hanna Luze Simon Schwingenschuh Thomas Birngruber Christoph Magnes Selma I. Mautner Lars-Peter Kamolz Petra Kotzbeck A novel human ex vivo skin model to study early local responses to burn injuries
description	Abstract Burn injuries initiate numerous processes such as heat shock response, inflammation and tissue regeneration. Reliable burn models are needed to elucidate the exact sequence of local events to be able to better predict when local inflammation triggers systemic inflammatory processes. In contrast to other ex vivo skin culture approaches, we used fresh abdominal skin explants to introduce contact burn injuries. Histological and ultrastructural analyses confirmed a partial-thickness burn pathology. Gene expression patterns and cytokine production profiles of key mediators of the local inflammation, heat shock response, and tissue regeneration were analyzed for 24 h after burn injury. We found significantly increased expression of factors involved in tissue regeneration and inflammation soon after burn injury. To investigate purely inflammation-mediated reactions we injected lipopolysaccharide into the dermis. In comparison to burn injury, lipopolysaccharide injection initiated an inflammatory response while expression patterns of heat shock and tissue regeneration genes were unaffected for the duration of the experiment. This novel ex vivo human skin model is suitable to study the local, early responses to skin injuries such as burns while maintaining an intact overall tissue structure and it gives valuable insights into local mechanisms at the very beginning of the wound healing process after burn injuries.
format	article
author	Elisabeth Hofmann Julia Fink Anita Eberl Eva-Maria Prugger Dagmar Kolb Hanna Luze Simon Schwingenschuh Thomas Birngruber Christoph Magnes Selma I. Mautner Lars-Peter Kamolz Petra Kotzbeck
author_facet	Elisabeth Hofmann Julia Fink Anita Eberl Eva-Maria Prugger Dagmar Kolb Hanna Luze Simon Schwingenschuh Thomas Birngruber Christoph Magnes Selma I. Mautner Lars-Peter Kamolz Petra Kotzbeck
author_sort	Elisabeth Hofmann
title	A novel human ex vivo skin model to study early local responses to burn injuries
title_short	A novel human ex vivo skin model to study early local responses to burn injuries
title_full	A novel human ex vivo skin model to study early local responses to burn injuries
title_fullStr	A novel human ex vivo skin model to study early local responses to burn injuries
title_full_unstemmed	A novel human ex vivo skin model to study early local responses to burn injuries
title_sort	novel human ex vivo skin model to study early local responses to burn injuries
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aa79ae995bc14dc48e7d3647aad13c78
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A novel human ex vivo skin model to study early local responses to burn injuries

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