$Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol$
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Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol

The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression of drug resistant and anti-apoptotic proteins in tumor cells. In a rescue protocol we evaluated the ability of toceranib phosphate concurrent with lomustine (CCNU) or L-asparaginase and vincristine to dec...

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Autores principales:	Sirivisoot Sirintra, Teewasutrakul Patharakrit, Rungsipipat Anudep, Tangkawattana Sirikachorn, Techangamsuwan Somporn
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	abcb1 abcg2 bax bcl2 relapsed/refractory lymphoma Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/aa8a9d264be6401f91881cf553ba45af
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spelling	oai:doaj.org-article:aa8a9d264be6401f91881cf553ba45af2021-11-17T21:27:52ZTranscriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol1820-744810.2478/acve-2018-0002https://doaj.org/article/aa8a9d264be6401f91881cf553ba45af2018-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0002https://doaj.org/toc/1820-7448The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression of drug resistant and anti-apoptotic proteins in tumor cells. In a rescue protocol we evaluated the ability of toceranib phosphate concurrent with lomustine (CCNU) or L-asparaginase and vincristine to decrease drug resistant and apoptotic proteins in relapsed and refractory canine lymphomas. The peripheral blood samples were collected before and after the rescue treatment from fourteen dogs that were refractory to cyclophosphamide-vincristine-prednisolone (COP) or COP-doxorubicin (CHOP) treatment and had recurrent multicentric lymphoma. The mRNA expression level of ABCB1, ABCG2, Bcl2 and Bax were determined by quantitative real-time PCR. The fold-change in ABCB1, ABCG2, Bcl2 and Bax mRNA levels were analyzed in correlation with the progression-free survival (PFS). After the rescue treatment, the ABCB1 and ABCG2 mRNA expression levels were 1.57- and 1.85-fold lower (p = 0.4 and p = 0.87), respectively, compared to pre-treatment. Bcl2/Bax ratio was numerically but not significantly decreased 1.02-fold (p = 0.74). The overall response rate of this protocol was 50% with a median PFS of 79 days (range 14-207 days). The low medians of relative expression levels of ABCB1, ABCG2 and Bcl2/Bax ratio group did not correlate with the clinical outcomes when compared to the high medians of relative expression levels, and likewise with the clinical stage, immunophenotype, histological grade and sub-stage. Therefore, the administration of a rescue drug with toceranib phosphate might be beneficial in refractory and relapsed canine lymphoma.Sirivisoot SirintraTeewasutrakul PatharakritRungsipipat AnudepTangkawattana SirikachornTechangamsuwan SompornSciendoarticleabcb1abcg2baxbcl2relapsed/refractory lymphomaVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 1, Pp 16-31 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	abcb1 abcg2 bax bcl2 relapsed/refractory lymphoma Veterinary medicine SF600-1100
spellingShingle	abcb1 abcg2 bax bcl2 relapsed/refractory lymphoma Veterinary medicine SF600-1100 Sirivisoot Sirintra Teewasutrakul Patharakrit Rungsipipat Anudep Tangkawattana Sirikachorn Techangamsuwan Somporn Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
description	The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression of drug resistant and anti-apoptotic proteins in tumor cells. In a rescue protocol we evaluated the ability of toceranib phosphate concurrent with lomustine (CCNU) or L-asparaginase and vincristine to decrease drug resistant and apoptotic proteins in relapsed and refractory canine lymphomas. The peripheral blood samples were collected before and after the rescue treatment from fourteen dogs that were refractory to cyclophosphamide-vincristine-prednisolone (COP) or COP-doxorubicin (CHOP) treatment and had recurrent multicentric lymphoma. The mRNA expression level of ABCB1, ABCG2, Bcl2 and Bax were determined by quantitative real-time PCR. The fold-change in ABCB1, ABCG2, Bcl2 and Bax mRNA levels were analyzed in correlation with the progression-free survival (PFS). After the rescue treatment, the ABCB1 and ABCG2 mRNA expression levels were 1.57- and 1.85-fold lower (p = 0.4 and p = 0.87), respectively, compared to pre-treatment. Bcl2/Bax ratio was numerically but not significantly decreased 1.02-fold (p = 0.74). The overall response rate of this protocol was 50% with a median PFS of 79 days (range 14-207 days). The low medians of relative expression levels of ABCB1, ABCG2 and Bcl2/Bax ratio group did not correlate with the clinical outcomes when compared to the high medians of relative expression levels, and likewise with the clinical stage, immunophenotype, histological grade and sub-stage. Therefore, the administration of a rescue drug with toceranib phosphate might be beneficial in refractory and relapsed canine lymphoma.
format	article
author	Sirivisoot Sirintra Teewasutrakul Patharakrit Rungsipipat Anudep Tangkawattana Sirikachorn Techangamsuwan Somporn
author_facet	Sirivisoot Sirintra Teewasutrakul Patharakrit Rungsipipat Anudep Tangkawattana Sirikachorn Techangamsuwan Somporn
author_sort	Sirivisoot Sirintra
title	Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
title_short	Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
title_full	Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
title_fullStr	Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
title_full_unstemmed	Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
title_sort	transcriptome analysis of abcb1, abcg2 and the bcl2/bax ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/aa8a9d264be6401f91881cf553ba45af
work_keys_str_mv	AT sirivisootsirintra transcriptomeanalysisofabcb1abcg2andthebcl2baxratioinrefractoryandrelapsedcaninelymphomasundertreatmentandrescueprotocol AT teewasutrakulpatharakrit transcriptomeanalysisofabcb1abcg2andthebcl2baxratioinrefractoryandrelapsedcaninelymphomasundertreatmentandrescueprotocol AT rungsipipatanudep transcriptomeanalysisofabcb1abcg2andthebcl2baxratioinrefractoryandrelapsedcaninelymphomasundertreatmentandrescueprotocol AT tangkawattanasirikachorn transcriptomeanalysisofabcb1abcg2andthebcl2baxratioinrefractoryandrelapsedcaninelymphomasundertreatmentandrescueprotocol AT techangamsuwansomporn transcriptomeanalysisofabcb1abcg2andthebcl2baxratioinrefractoryandrelapsedcaninelymphomasundertreatmentandrescueprotocol
_version_	1718425305948880896

Transcriptome analysis of ABCB1, ABCG2 and the BCL2/BAX ratio in refractory and relapsed canine lymphomas under treatment and rescue protocol

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