Molecular detection of porcine circovirus type 3 in Shanxi Province, China

Abstract Porcine circovirus type 3 (PCV3), which was first detected in the United States of America in 2015, is a potential threat to the swine industry. However, the prevalence of PCV3 in Shanxi Province, China, is unclear. In this research, the prevalence and genetic diversity of PCV3 were investi...

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Autores principales:	Weidong Yue, Yihui Liu, Xinrong Zhang, Haili Ma, Junping He
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Circovirus Prevalence Porcine circovirus type 3 (PCV3) Phylogenetic analysis Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/aa8ab7127fcd44d99f6cb294f0480df0
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spelling	oai:doaj.org-article:aa8ab7127fcd44d99f6cb294f0480df02021-11-14T12:06:17ZMolecular detection of porcine circovirus type 3 in Shanxi Province, China10.1186/s44149-021-00008-62731-0442https://doaj.org/article/aa8ab7127fcd44d99f6cb294f0480df02021-06-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00008-6https://doaj.org/toc/2731-0442Abstract Porcine circovirus type 3 (PCV3), which was first detected in the United States of America in 2015, is a potential threat to the swine industry. However, the prevalence of PCV3 in Shanxi Province, China, is unclear. In this research, the prevalence and genetic diversity of PCV3 were investigated in above area. Lung tissue samples (n = 491) from 19 pig slaughterhouses across 11 cities throughout Shanxi Province were analyzed for PCV3 infection by PCR in 2019. The results showed that PCV3 positive rates in slaughterhouses and individuals were 100% (19/19) and 86.76% (426/491), respectively. PCV2 and PCV3 double-positive rates in slaughterhouses and individuals were 100% (19/19) and 59.27% (291/491), respectively. PCR positive samples were further sequenced and 8 PCV3 isolates were identified. The nucleotide homology of these isolates with other PCV3 isolates in NCBI database was 97.45–99.90%. A phylogenetic analysis, based on the complete genomic sequence and ORF2, divided these PCV3 strains into 2 major groups. Based on A24/V and R27/K amino acid mutations of capsid protein, the 8 identified PCV3 strains were separated to 2 clades. This was the first detailed investigation into the epidemiology of PCV3 in Shanxi Province. Our findings enabled us to assess the possibility of widespread transmission from this region. Thus, current findings establish a basis for further studies of genetic variations in PCV3 strains circulating in China.Weidong YueYihui LiuXinrong ZhangHaili MaJunping HeBMCarticleCircovirusPrevalencePorcine circovirus type 3 (PCV3)Phylogenetic analysisVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Circovirus Prevalence Porcine circovirus type 3 (PCV3) Phylogenetic analysis Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Circovirus Prevalence Porcine circovirus type 3 (PCV3) Phylogenetic analysis Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Weidong Yue Yihui Liu Xinrong Zhang Haili Ma Junping He Molecular detection of porcine circovirus type 3 in Shanxi Province, China
description	Abstract Porcine circovirus type 3 (PCV3), which was first detected in the United States of America in 2015, is a potential threat to the swine industry. However, the prevalence of PCV3 in Shanxi Province, China, is unclear. In this research, the prevalence and genetic diversity of PCV3 were investigated in above area. Lung tissue samples (n = 491) from 19 pig slaughterhouses across 11 cities throughout Shanxi Province were analyzed for PCV3 infection by PCR in 2019. The results showed that PCV3 positive rates in slaughterhouses and individuals were 100% (19/19) and 86.76% (426/491), respectively. PCV2 and PCV3 double-positive rates in slaughterhouses and individuals were 100% (19/19) and 59.27% (291/491), respectively. PCR positive samples were further sequenced and 8 PCV3 isolates were identified. The nucleotide homology of these isolates with other PCV3 isolates in NCBI database was 97.45–99.90%. A phylogenetic analysis, based on the complete genomic sequence and ORF2, divided these PCV3 strains into 2 major groups. Based on A24/V and R27/K amino acid mutations of capsid protein, the 8 identified PCV3 strains were separated to 2 clades. This was the first detailed investigation into the epidemiology of PCV3 in Shanxi Province. Our findings enabled us to assess the possibility of widespread transmission from this region. Thus, current findings establish a basis for further studies of genetic variations in PCV3 strains circulating in China.
format	article
author	Weidong Yue Yihui Liu Xinrong Zhang Haili Ma Junping He
author_facet	Weidong Yue Yihui Liu Xinrong Zhang Haili Ma Junping He
author_sort	Weidong Yue
title	Molecular detection of porcine circovirus type 3 in Shanxi Province, China
title_short	Molecular detection of porcine circovirus type 3 in Shanxi Province, China
title_full	Molecular detection of porcine circovirus type 3 in Shanxi Province, China
title_fullStr	Molecular detection of porcine circovirus type 3 in Shanxi Province, China
title_full_unstemmed	Molecular detection of porcine circovirus type 3 in Shanxi Province, China
title_sort	molecular detection of porcine circovirus type 3 in shanxi province, china
publisher	BMC
publishDate	2021
url	https://doaj.org/article/aa8ab7127fcd44d99f6cb294f0480df0
work_keys_str_mv	AT weidongyue moleculardetectionofporcinecircovirustype3inshanxiprovincechina AT yihuiliu moleculardetectionofporcinecircovirustype3inshanxiprovincechina AT xinrongzhang moleculardetectionofporcinecircovirustype3inshanxiprovincechina AT hailima moleculardetectionofporcinecircovirustype3inshanxiprovincechina AT junpinghe moleculardetectionofporcinecircovirustype3inshanxiprovincechina
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Molecular detection of porcine circovirus type 3 in Shanxi Province, China

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