Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state

Abstract In the field of regenerative medicine, microcarriers are used as support matrix for the growth of adherent cells. They are increasingly recognised as promising biomaterials for large scale, cost-effective cell expansion bioreactor processes. However, their individual morphologies can be hig...

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Autores principales:	Sébastien de Bournonville, Liesbet Geris, Greet Kerckhofs
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa8c6d13d5bc4f2cb71a0711fb56805e
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spelling	oai:doaj.org-article:aa8c6d13d5bc4f2cb71a0711fb56805e2021-12-02T10:44:08ZMicro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state10.1038/s41598-021-81998-82045-2322https://doaj.org/article/aa8c6d13d5bc4f2cb71a0711fb56805e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81998-8https://doaj.org/toc/2045-2322Abstract In the field of regenerative medicine, microcarriers are used as support matrix for the growth of adherent cells. They are increasingly recognised as promising biomaterials for large scale, cost-effective cell expansion bioreactor processes. However, their individual morphologies can be highly heterogeneous which increases bioprocesses’ variability. Additionally, only limited information is available on the microcarriers’ 3D morphology and how it affects cell proliferation. Most imaging modalities do not provide sufficient 3D information or have a too limited field of view to appropriately study the 3D morphology. While microfocus X-ray computed tomography (microCT) could be appropriate, many microcarriers are hydrated before in-vitro use. This wet state makes them swell, changing considerably their morphology and making them indistinguishable from the culture solution in regular microCT images due to their physical density close to water. The use of contrast-enhanced microCT (CE-CT) has been recently reported for 3D imaging of soft materials. In this study, we selected a range of commercially available microcarrier types and used a combination of microCT and CE-CT for full 3D morphological characterization of large numbers of microcarriers, both in their dry and wet state. With in-house developed image processing and analysis tools, morphometrics of individual microcarriers were collected. Also, the morphology in wet state was assessed and related to accessible attachment surface area as a function of cell size. The morphological information on all microcarriers was collected in a publicly available database. This work provides a quantitative basis for optimization and modelling of microcarrier based cell expansion processes.Sébastien de BournonvilleLiesbet GerisGreet KerckhofsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sébastien de Bournonville Liesbet Geris Greet Kerckhofs Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
description	Abstract In the field of regenerative medicine, microcarriers are used as support matrix for the growth of adherent cells. They are increasingly recognised as promising biomaterials for large scale, cost-effective cell expansion bioreactor processes. However, their individual morphologies can be highly heterogeneous which increases bioprocesses’ variability. Additionally, only limited information is available on the microcarriers’ 3D morphology and how it affects cell proliferation. Most imaging modalities do not provide sufficient 3D information or have a too limited field of view to appropriately study the 3D morphology. While microfocus X-ray computed tomography (microCT) could be appropriate, many microcarriers are hydrated before in-vitro use. This wet state makes them swell, changing considerably their morphology and making them indistinguishable from the culture solution in regular microCT images due to their physical density close to water. The use of contrast-enhanced microCT (CE-CT) has been recently reported for 3D imaging of soft materials. In this study, we selected a range of commercially available microcarrier types and used a combination of microCT and CE-CT for full 3D morphological characterization of large numbers of microcarriers, both in their dry and wet state. With in-house developed image processing and analysis tools, morphometrics of individual microcarriers were collected. Also, the morphology in wet state was assessed and related to accessible attachment surface area as a function of cell size. The morphological information on all microcarriers was collected in a publicly available database. This work provides a quantitative basis for optimization and modelling of microcarrier based cell expansion processes.
format	article
author	Sébastien de Bournonville Liesbet Geris Greet Kerckhofs
author_facet	Sébastien de Bournonville Liesbet Geris Greet Kerckhofs
author_sort	Sébastien de Bournonville
title	Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
title_short	Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
title_full	Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
title_fullStr	Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
title_full_unstemmed	Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
title_sort	micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aa8c6d13d5bc4f2cb71a0711fb56805e
work_keys_str_mv	AT sebastiendebournonville microcomputedtomographywithandwithoutcontrastenhancementforthecharacterizationofmicrocarriersindryandwetstate AT liesbetgeris microcomputedtomographywithandwithoutcontrastenhancementforthecharacterizationofmicrocarriersindryandwetstate AT greetkerckhofs microcomputedtomographywithandwithoutcontrastenhancementforthecharacterizationofmicrocarriersindryandwetstate
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Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state

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