Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing

In recent years, lab-on-chip systems based on printed circuit board (PCB) substrates are gaining attraction primarily due to their low cost of manufacturing. Adapting inexpensive PCBs for development of immunosensors usually requires additional processing steps such as gold electroplating and electr...

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Autores principales:	Ruchira Nandeshwar, M. Santhosh Kumar, Kiran Kondabagil, Siddharth Tallur
Formato:	article
Lenguaje:	EN
Publicado:	IEEE 2021
Materias:	ENIG PCB immunosensor functionalization SARS-CoV-2 spike protein Electrical engineering. Electronics. Nuclear engineering TK1-9971
Acceso en línea:	https://doaj.org/article/aa8cc93dcbca4a66af9ca13702c7d6d5
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id	oai:doaj.org-article:aa8cc93dcbca4a66af9ca13702c7d6d5
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spelling	oai:doaj.org-article:aa8cc93dcbca4a66af9ca13702c7d6d52021-11-24T00:01:24ZElectrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing2169-353610.1109/ACCESS.2021.3128668https://doaj.org/article/aa8cc93dcbca4a66af9ca13702c7d6d52021-01-01T00:00:00Zhttps://ieeexplore.ieee.org/document/9617642/https://doaj.org/toc/2169-3536In recent years, lab-on-chip systems based on printed circuit board (PCB) substrates are gaining attraction primarily due to their low cost of manufacturing. Adapting inexpensive PCBs for development of immunosensors usually requires additional processing steps such as gold electroplating and electropolymerisation, that add to the manufacturing costs. In this work, we demonstrate methods to leverage electroless nickel immersion gold (ENIG) finish PCBs as electrodes for developing electrochemical immunosensors. We evaluated the performance of various parameters that impact sensor performance such as methods to clean impurities on PCB surface, optimization of redox probe concentration, and have successfully immobilized antibodies on the electrodes with cysteamine + glutaraldehyde aided process. Based on these methods, we demonstrate an application of ENIG finish PCB electrodes for detection of SARS-CoV-2 spike protein spiked in artificial saliva samples.Ruchira NandeshwarM. Santhosh KumarKiran KondabagilSiddharth TallurIEEEarticleENIG PCBimmunosensorfunctionalizationSARS-CoV-2spike proteinElectrical engineering. Electronics. Nuclear engineeringTK1-9971ENIEEE Access, Vol 9, Pp 154368-154377 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ENIG PCB immunosensor functionalization SARS-CoV-2 spike protein Electrical engineering. Electronics. Nuclear engineering TK1-9971
spellingShingle	ENIG PCB immunosensor functionalization SARS-CoV-2 spike protein Electrical engineering. Electronics. Nuclear engineering TK1-9971 Ruchira Nandeshwar M. Santhosh Kumar Kiran Kondabagil Siddharth Tallur Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing
description	In recent years, lab-on-chip systems based on printed circuit board (PCB) substrates are gaining attraction primarily due to their low cost of manufacturing. Adapting inexpensive PCBs for development of immunosensors usually requires additional processing steps such as gold electroplating and electropolymerisation, that add to the manufacturing costs. In this work, we demonstrate methods to leverage electroless nickel immersion gold (ENIG) finish PCBs as electrodes for developing electrochemical immunosensors. We evaluated the performance of various parameters that impact sensor performance such as methods to clean impurities on PCB surface, optimization of redox probe concentration, and have successfully immobilized antibodies on the electrodes with cysteamine + glutaraldehyde aided process. Based on these methods, we demonstrate an application of ENIG finish PCB electrodes for detection of SARS-CoV-2 spike protein spiked in artificial saliva samples.
format	article
author	Ruchira Nandeshwar M. Santhosh Kumar Kiran Kondabagil Siddharth Tallur
author_facet	Ruchira Nandeshwar M. Santhosh Kumar Kiran Kondabagil Siddharth Tallur
author_sort	Ruchira Nandeshwar
title	Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing
title_short	Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing
title_full	Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing
title_fullStr	Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing
title_full_unstemmed	Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing
title_sort	electrochemical immunosensor platform using low-cost enig pcb finish electrodes: application for sars-cov-2 spike protein sensing
publisher	IEEE
publishDate	2021
url	https://doaj.org/article/aa8cc93dcbca4a66af9ca13702c7d6d5
work_keys_str_mv	AT ruchiranandeshwar electrochemicalimmunosensorplatformusinglowcostenigpcbfinishelectrodesapplicationforsarscov2spikeproteinsensing AT msanthoshkumar electrochemicalimmunosensorplatformusinglowcostenigpcbfinishelectrodesapplicationforsarscov2spikeproteinsensing AT kirankondabagil electrochemicalimmunosensorplatformusinglowcostenigpcbfinishelectrodesapplicationforsarscov2spikeproteinsensing AT siddharthtallur electrochemicalimmunosensorplatformusinglowcostenigpcbfinishelectrodesapplicationforsarscov2spikeproteinsensing
_version_	1718416113855889408

Electrochemical Immunosensor Platform Using Low-Cost ENIG PCB Finish Electrodes: Application for SARS-CoV-2 Spike Protein Sensing

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