The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress

Abstract Genetically improved farmed tilapia (GIFT, Oreochromis niloticus) are commercially important fish that are cultured in China. GIFT are highly susceptible to diseases when exposed to high temperatures in summer. Better understanding the GIFT regulatory response to heat stress will not only h...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Qiang, Wen J. Bao, Fan Y. Tao, Jie He, Xia H. Li, Pao Xu, Lan Y. Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aaa282f4efc847be8fb6c169cc4033e6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aaa282f4efc847be8fb6c169cc4033e6
record_format	dspace
spelling	oai:doaj.org-article:aaa282f4efc847be8fb6c169cc4033e62021-12-02T11:52:17ZThe expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress10.1038/s41598-017-09264-42045-2322https://doaj.org/article/aaa282f4efc847be8fb6c169cc4033e62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09264-4https://doaj.org/toc/2045-2322Abstract Genetically improved farmed tilapia (GIFT, Oreochromis niloticus) are commercially important fish that are cultured in China. GIFT are highly susceptible to diseases when exposed to high temperatures in summer. Better understanding the GIFT regulatory response to heat stress will not only help in determining the relationship between heat stress signalling pathways and adaption mechanisms, but will also contribute to breeding new high-temperature tolerant strains of GIFT. In this study, we built control (28 °C) and heat-treated (37.5 °C) groups, and extracted RNA from the liver tissues for high-throughput next-generation sequencing to study the miRNA and mRNA expression profiles. We identified 28 differentially expressed (DE) miRNAs and 744 DE mRNAs between the control and heat-treated groups and annotated them using the KEGG database. A total of 38 target genes were predicted for 21 of the DE miRNAs, including 64 negative miRNA–mRNA interactions. We verified 15 DE miRNA–mRNA pairs and 16 other DE mRNAs by quantitative real-time PCR. Important regulatory pathways involved in the early response of GIFT to heat stress included organism system, metabolism, and diseases. Our findings will facilitate the understanding of regulatory pathways affected by acute heat stress, which will help to better prevent heat damage to GIFT.Jun QiangWen J. BaoFan Y. TaoJie HeXia H. LiPao XuLan Y. SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Qiang Wen J. Bao Fan Y. Tao Jie He Xia H. Li Pao Xu Lan Y. Sun The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress
description	Abstract Genetically improved farmed tilapia (GIFT, Oreochromis niloticus) are commercially important fish that are cultured in China. GIFT are highly susceptible to diseases when exposed to high temperatures in summer. Better understanding the GIFT regulatory response to heat stress will not only help in determining the relationship between heat stress signalling pathways and adaption mechanisms, but will also contribute to breeding new high-temperature tolerant strains of GIFT. In this study, we built control (28 °C) and heat-treated (37.5 °C) groups, and extracted RNA from the liver tissues for high-throughput next-generation sequencing to study the miRNA and mRNA expression profiles. We identified 28 differentially expressed (DE) miRNAs and 744 DE mRNAs between the control and heat-treated groups and annotated them using the KEGG database. A total of 38 target genes were predicted for 21 of the DE miRNAs, including 64 negative miRNA–mRNA interactions. We verified 15 DE miRNA–mRNA pairs and 16 other DE mRNAs by quantitative real-time PCR. Important regulatory pathways involved in the early response of GIFT to heat stress included organism system, metabolism, and diseases. Our findings will facilitate the understanding of regulatory pathways affected by acute heat stress, which will help to better prevent heat damage to GIFT.
format	article
author	Jun Qiang Wen J. Bao Fan Y. Tao Jie He Xia H. Li Pao Xu Lan Y. Sun
author_facet	Jun Qiang Wen J. Bao Fan Y. Tao Jie He Xia H. Li Pao Xu Lan Y. Sun
author_sort	Jun Qiang
title	The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress
title_short	The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress
title_full	The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress
title_fullStr	The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress
title_full_unstemmed	The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress
title_sort	expression profiles of mirna–mrna of early response in genetically improved farmed tilapia (oreochromis niloticus) liver by acute heat stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/aaa282f4efc847be8fb6c169cc4033e6
work_keys_str_mv	AT junqiang theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT wenjbao theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT fanytao theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT jiehe theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT xiahli theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT paoxu theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT lanysun theexpressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT junqiang expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT wenjbao expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT fanytao expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT jiehe expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT xiahli expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT paoxu expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress AT lanysun expressionprofilesofmirnamrnaofearlyresponseingeneticallyimprovedfarmedtilapiaoreochromisniloticusliverbyacuteheatstress
_version_	1718395124440891392

The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress

Ejemplares similares