Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea
Accelerating genetic gain in crop improvement is required to ensure improved yield and yield stability under increasingly challenging climatic conditions. This case study demonstrates the effective confluence of innovative breeding technologies within a collaborative breeding framework to develop an...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:aaa9c37745074289a8f829223b554cc42021-12-03T05:20:07ZEvidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea1664-462X10.3389/fpls.2021.779122https://doaj.org/article/aaa9c37745074289a8f829223b554cc42021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.779122/fullhttps://doaj.org/toc/1664-462XAccelerating genetic gain in crop improvement is required to ensure improved yield and yield stability under increasingly challenging climatic conditions. This case study demonstrates the effective confluence of innovative breeding technologies within a collaborative breeding framework to develop and rapidly introgress imidazolinone Group 2 herbicide tolerance into an adapted Australian chickpea genetic background. A well-adapted, high-yielding desi cultivar PBA HatTrick was treated with ethyl methanesulfonate to generate mutations in the ACETOHYDROXYACID SYNTHASE 1 (CaAHAS1) gene. After 2 years of field screening with imidazolinone herbicide across >20 ha and controlled environment progeny screening, two selections were identified which exhibited putative herbicide tolerance. Both selections contained the same single amino acid substitution, from alanine to valine at position 205 (A205V) in the AHAS1 protein, and KASP™ markers were developed to discriminate between tolerant and intolerant genotypes. A pipeline combining conventional crossing and F2 production with accelerated single seed descent from F2:4 and marker-assisted selection at F2 rapidly introgressed the herbicide tolerance trait from one of the mutant selections, D15PAHI002, into PBA Seamer, a desi cultivar adapted to Australian cropping areas. Field evaluation of the derivatives of the D15PAHI002 × PBA Seamer cross was analyzed using a factor analytic mixed model statistical approach designed to accommodate low seed numbers resulting from accelerated single seed descent. To further accelerate trait introgression, field evaluation trials were undertaken concurrent with crop safety testing trials. In 2020, 4 years after the initial cross, an advanced line selection CBA2061, bearing acetohydroxyacid synthase (AHAS) inhibitor tolerance and agronomic and disease resistance traits comparable to parent PBA Seamer, was entered into Australian National Variety Trials as a precursor to cultivar registration. The combination of cross-institutional collaboration and the application of novel pre-breeding platforms and statistical technologies facilitated a 3-year saving compared to a traditional breeding approach. This breeding pipeline can be used as a model to accelerate genetic gain in other self-pollinating species, particularly food legumes.Janine CroserJanine CroserDili MaoDili MaoNicole DronSimon MichelmoreSimon MichelmoreLarn McMurrayChristopher PrestonDylan BruceFrancis Chuks OgbonnayaFederico Martin RibaltaJulie HayesJudith LichtenzveigWilliam ErskineBrian CullisTim SuttonTim SuttonKristy HobsonFrontiers Media S.A.articlemutationimidazolinonesulfonylureamarker-assisted selection (MAS)accelerated single seed descentsparse phenotypingPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| topic |
mutation imidazolinone sulfonylurea marker-assisted selection (MAS) accelerated single seed descent sparse phenotyping Plant culture SB1-1110 |
| spellingShingle |
mutation imidazolinone sulfonylurea marker-assisted selection (MAS) accelerated single seed descent sparse phenotyping Plant culture SB1-1110 Janine Croser Janine Croser Dili Mao Dili Mao Nicole Dron Simon Michelmore Simon Michelmore Larn McMurray Christopher Preston Dylan Bruce Francis Chuks Ogbonnaya Federico Martin Ribalta Julie Hayes Judith Lichtenzveig William Erskine Brian Cullis Tim Sutton Tim Sutton Kristy Hobson Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea |
| description |
Accelerating genetic gain in crop improvement is required to ensure improved yield and yield stability under increasingly challenging climatic conditions. This case study demonstrates the effective confluence of innovative breeding technologies within a collaborative breeding framework to develop and rapidly introgress imidazolinone Group 2 herbicide tolerance into an adapted Australian chickpea genetic background. A well-adapted, high-yielding desi cultivar PBA HatTrick was treated with ethyl methanesulfonate to generate mutations in the ACETOHYDROXYACID SYNTHASE 1 (CaAHAS1) gene. After 2 years of field screening with imidazolinone herbicide across >20 ha and controlled environment progeny screening, two selections were identified which exhibited putative herbicide tolerance. Both selections contained the same single amino acid substitution, from alanine to valine at position 205 (A205V) in the AHAS1 protein, and KASP™ markers were developed to discriminate between tolerant and intolerant genotypes. A pipeline combining conventional crossing and F2 production with accelerated single seed descent from F2:4 and marker-assisted selection at F2 rapidly introgressed the herbicide tolerance trait from one of the mutant selections, D15PAHI002, into PBA Seamer, a desi cultivar adapted to Australian cropping areas. Field evaluation of the derivatives of the D15PAHI002 × PBA Seamer cross was analyzed using a factor analytic mixed model statistical approach designed to accommodate low seed numbers resulting from accelerated single seed descent. To further accelerate trait introgression, field evaluation trials were undertaken concurrent with crop safety testing trials. In 2020, 4 years after the initial cross, an advanced line selection CBA2061, bearing acetohydroxyacid synthase (AHAS) inhibitor tolerance and agronomic and disease resistance traits comparable to parent PBA Seamer, was entered into Australian National Variety Trials as a precursor to cultivar registration. The combination of cross-institutional collaboration and the application of novel pre-breeding platforms and statistical technologies facilitated a 3-year saving compared to a traditional breeding approach. This breeding pipeline can be used as a model to accelerate genetic gain in other self-pollinating species, particularly food legumes. |
| format |
article |
| author |
Janine Croser Janine Croser Dili Mao Dili Mao Nicole Dron Simon Michelmore Simon Michelmore Larn McMurray Christopher Preston Dylan Bruce Francis Chuks Ogbonnaya Federico Martin Ribalta Julie Hayes Judith Lichtenzveig William Erskine Brian Cullis Tim Sutton Tim Sutton Kristy Hobson |
| author_facet |
Janine Croser Janine Croser Dili Mao Dili Mao Nicole Dron Simon Michelmore Simon Michelmore Larn McMurray Christopher Preston Dylan Bruce Francis Chuks Ogbonnaya Federico Martin Ribalta Julie Hayes Judith Lichtenzveig William Erskine Brian Cullis Tim Sutton Tim Sutton Kristy Hobson |
| author_sort |
Janine Croser |
| title |
Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea |
| title_short |
Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea |
| title_full |
Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea |
| title_fullStr |
Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea |
| title_full_unstemmed |
Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea |
| title_sort |
evidence for the application of emerging technologies to accelerate crop improvement – a collaborative pipeline to introgress herbicide tolerance into chickpea |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/aaa9c37745074289a8f829223b554cc4 |
| work_keys_str_mv |
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