Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

R Olivera,1 LN Moro,2 R Jordan,1 N Pallarols,3 A Guglielminetti,3 C Luzzani,2 SG Miriuka,2 G Vichera1 1KHEIRON S.A Laboratory, Pilar, Buenos Aires, Argentina; 2LIAN-Unit Associated with CONICET, FLENI, Belen de Escobar, Buenos Aires, Argentina; 3Kawell Equine Hospital, Solís, Buenos Aires...

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Autores principales:	Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Equine Cloning MSC SCNT Cytology QH573-671
Acceso en línea:	https://doaj.org/article/aaacd7ef4ce24e17b97ab4ab8fa2d233
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spelling	oai:doaj.org-article:aaacd7ef4ce24e17b97ab4ab8fa2d2332021-12-02T05:12:03ZBone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses1178-6957https://doaj.org/article/aaacd7ef4ce24e17b97ab4ab8fa2d2332018-02-01T00:00:00Zhttps://www.dovepress.com/bone-marrow-mesenchymal-stem-cells-as-nuclear-donors-improve-viability-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957R Olivera,1 LN Moro,2 R Jordan,1 N Pallarols,3 A Guglielminetti,3 C Luzzani,2 SG Miriuka,2 G Vichera1 1KHEIRON S.A Laboratory, Pilar, Buenos Aires, Argentina; 2LIAN-Unit Associated with CONICET, FLENI, Belen de Escobar, Buenos Aires, Argentina; 3Kawell Equine Hospital, Solís, Buenos Aires, Argentina Introduction: Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem cells (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts. Materials and methods: Zona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination (AI) recovered by uterine flushing and transferred to recipient mares were used as controls (AI group). Results: Blastocyst development was higher in the MSC group than in the AF group (18.1% vs 10.9%, respectively; p<0.05). However, pregnancy rates and delivery rates were similar in both cloning groups, although they were lower than in the AI group (pregnancy rates: 17.7% [41/232] for MSC, 12.5% [37/297] for AF and 80.7% [71/88] for AI; delivery rates: 56.8% [21/37], 41.5% [17/41] and 90.1% [64/71], respectively). Remarkably, the gestation length of the AF group was significantly longer than the control (361.7±10.9 vs 333.9±8.7 days), in contrast to the MSC group (340.6±8.89 days). Of the total deliveries, 95.2% (20/21) of the MSC-foals were viable, compared to 52.9% (9/17) of the AF-foals (p<0.05). In addition, the AF-foals had more physiological abnormalities at birth than the MSC-foals; 90.5% (19/21) of the MSC-delivered foals were completely normal and healthy, compared to 35.3% (6/17) in the AF group. The abnormalities included flexural or angular limb deformities, umbilical cord enlargement, placental alterations and signs of syndrome of neonatal maladjustment, which were treated in most cases. Conclusion: In summary, we obtained 29 viable cloned foals and found that MSCs are suitable donor cells in horse cloning. Even more, these cells could be more efficiently reprogrammed compared to fibroblasts. Keywords: equine, cloning, MSC, SCNTOlivera RMoro LNJordan RPallarols NGuglielminetti ALuzzani CMiriuka SGVichera GDove Medical PressarticleEquineCloningMSCSCNTCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 11, Pp 13-22 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Equine Cloning MSC SCNT Cytology QH573-671
spellingShingle	Equine Cloning MSC SCNT Cytology QH573-671 Olivera R Moro LN Jordan R Pallarols N Guglielminetti A Luzzani C Miriuka SG Vichera G Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
description	R Olivera,1 LN Moro,2 R Jordan,1 N Pallarols,3 A Guglielminetti,3 C Luzzani,2 SG Miriuka,2 G Vichera1 1KHEIRON S.A Laboratory, Pilar, Buenos Aires, Argentina; 2LIAN-Unit Associated with CONICET, FLENI, Belen de Escobar, Buenos Aires, Argentina; 3Kawell Equine Hospital, Solís, Buenos Aires, Argentina Introduction: Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem cells (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts. Materials and methods: Zona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination (AI) recovered by uterine flushing and transferred to recipient mares were used as controls (AI group). Results: Blastocyst development was higher in the MSC group than in the AF group (18.1% vs 10.9%, respectively; p<0.05). However, pregnancy rates and delivery rates were similar in both cloning groups, although they were lower than in the AI group (pregnancy rates: 17.7% [41/232] for MSC, 12.5% [37/297] for AF and 80.7% [71/88] for AI; delivery rates: 56.8% [21/37], 41.5% [17/41] and 90.1% [64/71], respectively). Remarkably, the gestation length of the AF group was significantly longer than the control (361.7±10.9 vs 333.9±8.7 days), in contrast to the MSC group (340.6±8.89 days). Of the total deliveries, 95.2% (20/21) of the MSC-foals were viable, compared to 52.9% (9/17) of the AF-foals (p<0.05). In addition, the AF-foals had more physiological abnormalities at birth than the MSC-foals; 90.5% (19/21) of the MSC-delivered foals were completely normal and healthy, compared to 35.3% (6/17) in the AF group. The abnormalities included flexural or angular limb deformities, umbilical cord enlargement, placental alterations and signs of syndrome of neonatal maladjustment, which were treated in most cases. Conclusion: In summary, we obtained 29 viable cloned foals and found that MSCs are suitable donor cells in horse cloning. Even more, these cells could be more efficiently reprogrammed compared to fibroblasts. Keywords: equine, cloning, MSC, SCNT
format	article
author	Olivera R Moro LN Jordan R Pallarols N Guglielminetti A Luzzani C Miriuka SG Vichera G
author_facet	Olivera R Moro LN Jordan R Pallarols N Guglielminetti A Luzzani C Miriuka SG Vichera G
author_sort	Olivera R
title	Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
title_short	Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
title_full	Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
title_fullStr	Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
title_full_unstemmed	Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
title_sort	bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/aaacd7ef4ce24e17b97ab4ab8fa2d233
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Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

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