Tumor cell plasticity and angiogenesis in human melanomas.

Recent molecular studies provide evidence for a significant transcriptional plasticity of tumor cell subpopulations that facilitate an active contribution to tumor vasculature. This feature is accompanied by morphological changes both in vitro and in vivo. Herein, we investigated the morphological p...

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Autores principales:	Daniela Mihic-Probst, Kristian Ikenberg, Marianne Tinguely, Peter Schraml, Silvia Behnke, Burkhardt Seifert, Gianluca Civenni, Lukas Sommer, Holger Moch, Reinhard Dummer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aabace52fadd4ab8a51b44c2d30507ef
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spelling	oai:doaj.org-article:aabace52fadd4ab8a51b44c2d30507ef2021-11-18T07:24:48ZTumor cell plasticity and angiogenesis in human melanomas.1932-620310.1371/journal.pone.0033571https://doaj.org/article/aabace52fadd4ab8a51b44c2d30507ef2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22442699/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent molecular studies provide evidence for a significant transcriptional plasticity of tumor cell subpopulations that facilitate an active contribution to tumor vasculature. This feature is accompanied by morphological changes both in vitro and in vivo. Herein, we investigated the morphological plasticity of tumor cells with special focus on vasculogenic mimicry and neovascularisation in human melanoma and mouse xenografts of human melanoma cell lines. In melanoma xenograft experiments, different vessel markers and green fluorescent protein expression were used to show how melanoma cells contribute to neovascularization. Additionally, we analyzed neovascularization in 49 primary melanomas and 175 melanoma metastases using immunostaining for blood (CD34) and lymphatic (D2-40) vessel-specific markers. We found significantly more lymphatic vessels in primary melanomas than in melanoma metastases (p<0.0001). In contrast to the near absence of lymphatic vessels within metastases, we found extensive blood micro-neovascularization. Blood micro-neovascularization was absent in micro metastases (less than 2 mm). A significant inverse correlation between Glut-1 expression (implying local hypoxia) and the presence of microvessels indicates their functional activity as blood vessels (p<0.0001). We suggest that the hypoxic microenvironment in metastases contributes to a phenotype switch allowing melanoma cells to physically contribute to blood vessel formation.Daniela Mihic-ProbstKristian IkenbergMarianne TinguelyPeter SchramlSilvia BehnkeBurkhardt SeifertGianluca CivenniLukas SommerHolger MochReinhard DummerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33571 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Mihic-Probst Kristian Ikenberg Marianne Tinguely Peter Schraml Silvia Behnke Burkhardt Seifert Gianluca Civenni Lukas Sommer Holger Moch Reinhard Dummer Tumor cell plasticity and angiogenesis in human melanomas.
description	Recent molecular studies provide evidence for a significant transcriptional plasticity of tumor cell subpopulations that facilitate an active contribution to tumor vasculature. This feature is accompanied by morphological changes both in vitro and in vivo. Herein, we investigated the morphological plasticity of tumor cells with special focus on vasculogenic mimicry and neovascularisation in human melanoma and mouse xenografts of human melanoma cell lines. In melanoma xenograft experiments, different vessel markers and green fluorescent protein expression were used to show how melanoma cells contribute to neovascularization. Additionally, we analyzed neovascularization in 49 primary melanomas and 175 melanoma metastases using immunostaining for blood (CD34) and lymphatic (D2-40) vessel-specific markers. We found significantly more lymphatic vessels in primary melanomas than in melanoma metastases (p<0.0001). In contrast to the near absence of lymphatic vessels within metastases, we found extensive blood micro-neovascularization. Blood micro-neovascularization was absent in micro metastases (less than 2 mm). A significant inverse correlation between Glut-1 expression (implying local hypoxia) and the presence of microvessels indicates their functional activity as blood vessels (p<0.0001). We suggest that the hypoxic microenvironment in metastases contributes to a phenotype switch allowing melanoma cells to physically contribute to blood vessel formation.
format	article
author	Daniela Mihic-Probst Kristian Ikenberg Marianne Tinguely Peter Schraml Silvia Behnke Burkhardt Seifert Gianluca Civenni Lukas Sommer Holger Moch Reinhard Dummer
author_facet	Daniela Mihic-Probst Kristian Ikenberg Marianne Tinguely Peter Schraml Silvia Behnke Burkhardt Seifert Gianluca Civenni Lukas Sommer Holger Moch Reinhard Dummer
author_sort	Daniela Mihic-Probst
title	Tumor cell plasticity and angiogenesis in human melanomas.
title_short	Tumor cell plasticity and angiogenesis in human melanomas.
title_full	Tumor cell plasticity and angiogenesis in human melanomas.
title_fullStr	Tumor cell plasticity and angiogenesis in human melanomas.
title_full_unstemmed	Tumor cell plasticity and angiogenesis in human melanomas.
title_sort	tumor cell plasticity and angiogenesis in human melanomas.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/aabace52fadd4ab8a51b44c2d30507ef
work_keys_str_mv	AT danielamihicprobst tumorcellplasticityandangiogenesisinhumanmelanomas AT kristianikenberg tumorcellplasticityandangiogenesisinhumanmelanomas AT mariannetinguely tumorcellplasticityandangiogenesisinhumanmelanomas AT peterschraml tumorcellplasticityandangiogenesisinhumanmelanomas AT silviabehnke tumorcellplasticityandangiogenesisinhumanmelanomas AT burkhardtseifert tumorcellplasticityandangiogenesisinhumanmelanomas AT gianlucacivenni tumorcellplasticityandangiogenesisinhumanmelanomas AT lukassommer tumorcellplasticityandangiogenesisinhumanmelanomas AT holgermoch tumorcellplasticityandangiogenesisinhumanmelanomas AT reinharddummer tumorcellplasticityandangiogenesisinhumanmelanomas
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Tumor cell plasticity and angiogenesis in human melanomas.

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