Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing

Abstract On August 14th, 2018, a Beijing resident living in Xicheng District found a female H. longicornis tick attached to the skin at the front of his upper shin. On examination, the patient was afebrile and appeared well. The species of the tick was identified through morphological characteristic...

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Autores principales:	Jizhou Lv, Huiyu Wang, Xueqing Han, Lin Mei, Xiangfen Yuan, Yufang Kong, Junhua Deng, Zhen F. Fu, Shaoqiang Wu, Xiangmei Lin
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Tick-borne pathogens Metagenomic analyses H. longicornis Downtown Beijing Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/aac2945f77204401b46089fb54dda131
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spelling	oai:doaj.org-article:aac2945f77204401b46089fb54dda1312021-11-14T12:06:09ZIdentification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing10.1186/s44149-021-00018-42731-0442https://doaj.org/article/aac2945f77204401b46089fb54dda1312021-09-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00018-4https://doaj.org/toc/2731-0442Abstract On August 14th, 2018, a Beijing resident living in Xicheng District found a female H. longicornis tick attached to the skin at the front of his upper shin. On examination, the patient was afebrile and appeared well. The species of the tick was identified through morphological characteristics and phylogenetic analysis based on cytochrome C oxidase subunit I. This H. longicornis tick was screened for tick-borne pathogens such as viruses, bacteria and parasites. RNA pathogens were screened by PCR and sequencing, while DNA pathogens were screened by metagenomic analyses. It was found that the tick was positive for the DNA sequences of zoonotic and animal pathogens such as A. phagocytophilum, Ehrlichia minasensis and C. burnetii. Considering the good health condition of the patient, we hypothesized that the pathogens originated from the tick specimen itself rather than host blood meal. For the first time, our study reveals the possible risk of transmission of tick-borne pathogens to human beings through tick bit in downtown Beijing. Further research is needed to screen for tick-borne pathogens among unfed ticks collected from central Beijing.Jizhou LvHuiyu WangXueqing HanLin MeiXiangfen YuanYufang KongJunhua DengZhen F. FuShaoqiang WuXiangmei LinBMCarticleTick-borne pathogensMetagenomic analysesH. longicornisDowntown BeijingVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tick-borne pathogens Metagenomic analyses H. longicornis Downtown Beijing Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Tick-borne pathogens Metagenomic analyses H. longicornis Downtown Beijing Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Jizhou Lv Huiyu Wang Xueqing Han Lin Mei Xiangfen Yuan Yufang Kong Junhua Deng Zhen F. Fu Shaoqiang Wu Xiangmei Lin Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
description	Abstract On August 14th, 2018, a Beijing resident living in Xicheng District found a female H. longicornis tick attached to the skin at the front of his upper shin. On examination, the patient was afebrile and appeared well. The species of the tick was identified through morphological characteristics and phylogenetic analysis based on cytochrome C oxidase subunit I. This H. longicornis tick was screened for tick-borne pathogens such as viruses, bacteria and parasites. RNA pathogens were screened by PCR and sequencing, while DNA pathogens were screened by metagenomic analyses. It was found that the tick was positive for the DNA sequences of zoonotic and animal pathogens such as A. phagocytophilum, Ehrlichia minasensis and C. burnetii. Considering the good health condition of the patient, we hypothesized that the pathogens originated from the tick specimen itself rather than host blood meal. For the first time, our study reveals the possible risk of transmission of tick-borne pathogens to human beings through tick bit in downtown Beijing. Further research is needed to screen for tick-borne pathogens among unfed ticks collected from central Beijing.
format	article
author	Jizhou Lv Huiyu Wang Xueqing Han Lin Mei Xiangfen Yuan Yufang Kong Junhua Deng Zhen F. Fu Shaoqiang Wu Xiangmei Lin
author_facet	Jizhou Lv Huiyu Wang Xueqing Han Lin Mei Xiangfen Yuan Yufang Kong Junhua Deng Zhen F. Fu Shaoqiang Wu Xiangmei Lin
author_sort	Jizhou Lv
title	Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
title_short	Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
title_full	Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
title_fullStr	Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
title_full_unstemmed	Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
title_sort	identification of tick-borne pathogens using metagenomic analyses in h. longicornis feeding on humans in downtown beijing
publisher	BMC
publishDate	2021
url	https://doaj.org/article/aac2945f77204401b46089fb54dda131
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Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing

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