Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers

Abstract We report a technique to reconstruct cardiovascular tissue using multiscale scaffolds incorporating polycaprolactone fibers with double-layered hydrogels comprising fibrin hydrogel surrounded by secondary alginate hydrogel. The scaffolds compartmentalized cells into the core region of cardi...

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Autores principales:	Yun-Min Kook, Soonjae Hwang, Hyerim Kim, Ki-Jong Rhee, Kangwon Lee, Won-Gun Koh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aacda3c9ecb54b908110acda8bd558c3
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spelling	oai:doaj.org-article:aacda3c9ecb54b908110acda8bd558c32021-12-02T12:34:05ZCardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers10.1038/s41598-020-77187-82045-2322https://doaj.org/article/aacda3c9ecb54b908110acda8bd558c32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77187-8https://doaj.org/toc/2045-2322Abstract We report a technique to reconstruct cardiovascular tissue using multiscale scaffolds incorporating polycaprolactone fibers with double-layered hydrogels comprising fibrin hydrogel surrounded by secondary alginate hydrogel. The scaffolds compartmentalized cells into the core region of cardiac tissue and the peripheral region of blood vessels to construct cardiovascular tissue, which was accomplished by a triple culture system of adipose-derived mesenchymal stem cells (ADSCs) with C2C12 myoblasts on polycaprolactone (PCL) fibers along with human umbilical vein endothelial cells (HUVECs) in fibrin hydrogel. The secondary alginate hydrogel prevented encapsulated cells from migrating outside scaffold and maintained the scaffold structure without distortion after subcutaneous implantation. According to in vitro studies, resultant scaffolds promoted new blood vessel formation as well as cardiomyogenic phenotype expression of ADSCs. Cardiac muscle-specific genes were expressed from stem cells and peripheral blood vessels from HUVECs were also successfully developed in subcutaneously implanted cell-laden multiscale scaffolds. Furthermore, the encapsulated stem cells modulated the immune response of scaffolds by secreting anti-inflammatory cytokines for successful tissue construction. Our study reveals that multiscale scaffolds can be promising for the remodeling and transplantation of cardiovascular tissue.Yun-Min KookSoonjae HwangHyerim KimKi-Jong RheeKangwon LeeWon-Gun KohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yun-Min Kook Soonjae Hwang Hyerim Kim Ki-Jong Rhee Kangwon Lee Won-Gun Koh Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
description	Abstract We report a technique to reconstruct cardiovascular tissue using multiscale scaffolds incorporating polycaprolactone fibers with double-layered hydrogels comprising fibrin hydrogel surrounded by secondary alginate hydrogel. The scaffolds compartmentalized cells into the core region of cardiac tissue and the peripheral region of blood vessels to construct cardiovascular tissue, which was accomplished by a triple culture system of adipose-derived mesenchymal stem cells (ADSCs) with C2C12 myoblasts on polycaprolactone (PCL) fibers along with human umbilical vein endothelial cells (HUVECs) in fibrin hydrogel. The secondary alginate hydrogel prevented encapsulated cells from migrating outside scaffold and maintained the scaffold structure without distortion after subcutaneous implantation. According to in vitro studies, resultant scaffolds promoted new blood vessel formation as well as cardiomyogenic phenotype expression of ADSCs. Cardiac muscle-specific genes were expressed from stem cells and peripheral blood vessels from HUVECs were also successfully developed in subcutaneously implanted cell-laden multiscale scaffolds. Furthermore, the encapsulated stem cells modulated the immune response of scaffolds by secreting anti-inflammatory cytokines for successful tissue construction. Our study reveals that multiscale scaffolds can be promising for the remodeling and transplantation of cardiovascular tissue.
format	article
author	Yun-Min Kook Soonjae Hwang Hyerim Kim Ki-Jong Rhee Kangwon Lee Won-Gun Koh
author_facet	Yun-Min Kook Soonjae Hwang Hyerim Kim Ki-Jong Rhee Kangwon Lee Won-Gun Koh
author_sort	Yun-Min Kook
title	Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
title_short	Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
title_full	Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
title_fullStr	Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
title_full_unstemmed	Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
title_sort	cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/aacda3c9ecb54b908110acda8bd558c3
work_keys_str_mv	AT yunminkook cardiovasculartissueregenerationsystembasedonmultiscalescaffoldscomprisingdoublelayeredhydrogelsandfibers AT soonjaehwang cardiovasculartissueregenerationsystembasedonmultiscalescaffoldscomprisingdoublelayeredhydrogelsandfibers AT hyerimkim cardiovasculartissueregenerationsystembasedonmultiscalescaffoldscomprisingdoublelayeredhydrogelsandfibers AT kijongrhee cardiovasculartissueregenerationsystembasedonmultiscalescaffoldscomprisingdoublelayeredhydrogelsandfibers AT kangwonlee cardiovasculartissueregenerationsystembasedonmultiscalescaffoldscomprisingdoublelayeredhydrogelsandfibers AT wongunkoh cardiovasculartissueregenerationsystembasedonmultiscalescaffoldscomprisingdoublelayeredhydrogelsandfibers
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Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers

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