Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

Ying Wang1, Yuxiang Han1, Yingying Yang1, Jingci Yang1, Xiaonan Guo1, Jingnan Zhang1, Ling Pan1, Guohua Xia2, Baoan Chen21Department of Hematology, the 2nd Hospital of Hebei Medical University, People&rsquo;s Republic of China; 2Department of Hematology, the Affiliated Zhongda Hospital, Medi...

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Autores principales:	Wang Y, Han Y, Yang Y, Yang J, Guo X, Zhang J, Pan L, Xia G, Chen B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/aad59e0ca3024626ae5ca455ae3e73ad
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spelling	oai:doaj.org-article:aad59e0ca3024626ae5ca455ae3e73ad2021-12-02T05:09:58ZEffect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells1176-91141178-2013https://doaj.org/article/aad59e0ca3024626ae5ca455ae3e73ad2011-06-01T00:00:00Zhttp://www.dovepress.com/effect-of-interaction-of-magnetic-nanoparticles-of-fe3o4-and-artesunat-a7625https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ying Wang1, Yuxiang Han1, Yingying Yang1, Jingci Yang1, Xiaonan Guo1, Jingnan Zhang1, Ling Pan1, Guohua Xia2, Baoan Chen21Department of Hematology, the 2nd Hospital of Hebei Medical University, People&rsquo;s Republic of China; 2Department of Hematology, the Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, People&rsquo;s Republic of ChinaAbstract: The present study evaluated whether the magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe3O4 on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot. After being incubated with the copolymer of ART with MNPs-Fe3O4 for 48 hours, the growth inhibition rate of K562 cells was significantly increased compared with that of K562 cells treated with ART alone (P &lt; 0.05), and the apoptosis rate of K562 cells was increased significantly compared with that of K562 cells treated with ART alone, suggesting that MNPs-Fe3O4 can enhance the activity of ART. Interestingly, the copolymer-induced cell death was attenuated by caspase inhibitor Z-VAD-FMK. Our results also showed that treatment with the copolymer of MNPs-Fe3O4 and ART increased the expression of bcl-2, bax, bcl-rambo, and caspase-3 proteins, and decreased the expression of survivin protein in K562 cells compared with ART treatment alone. These results suggest that MNPs-Fe3O4 can enhance ART-induced apoptosis, which may be related to the upregulation of bcl-rambo and downregulation of survivin.Keywords: magnetic nanoparticles, MNPs-Fe3O4, artesunate, K562 cells, apoptosis, bcl-ramboWang YHan YYang YYang JGuo XZhang JPan LXia GChen BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1185-1192 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang Y Han Y Yang Y Yang J Guo X Zhang J Pan L Xia G Chen B Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
description	Ying Wang1, Yuxiang Han1, Yingying Yang1, Jingci Yang1, Xiaonan Guo1, Jingnan Zhang1, Ling Pan1, Guohua Xia2, Baoan Chen21Department of Hematology, the 2nd Hospital of Hebei Medical University, People&rsquo;s Republic of China; 2Department of Hematology, the Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, People&rsquo;s Republic of ChinaAbstract: The present study evaluated whether the magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe3O4 on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot. After being incubated with the copolymer of ART with MNPs-Fe3O4 for 48 hours, the growth inhibition rate of K562 cells was significantly increased compared with that of K562 cells treated with ART alone (P &lt; 0.05), and the apoptosis rate of K562 cells was increased significantly compared with that of K562 cells treated with ART alone, suggesting that MNPs-Fe3O4 can enhance the activity of ART. Interestingly, the copolymer-induced cell death was attenuated by caspase inhibitor Z-VAD-FMK. Our results also showed that treatment with the copolymer of MNPs-Fe3O4 and ART increased the expression of bcl-2, bax, bcl-rambo, and caspase-3 proteins, and decreased the expression of survivin protein in K562 cells compared with ART treatment alone. These results suggest that MNPs-Fe3O4 can enhance ART-induced apoptosis, which may be related to the upregulation of bcl-rambo and downregulation of survivin.Keywords: magnetic nanoparticles, MNPs-Fe3O4, artesunate, K562 cells, apoptosis, bcl-rambo
format	article
author	Wang Y Han Y Yang Y Yang J Guo X Zhang J Pan L Xia G Chen B
author_facet	Wang Y Han Y Yang Y Yang J Guo X Zhang J Pan L Xia G Chen B
author_sort	Wang Y
title	Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
title_short	Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
title_full	Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
title_fullStr	Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
title_full_unstemmed	Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
title_sort	effect of interaction of magnetic nanoparticles of fe3o4 and artesunate on apoptosis of k562 cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/aad59e0ca3024626ae5ca455ae3e73ad
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Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

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