Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia

Abstract. Wuhan YOP, Haryanto A, Tjahajati I. 2020. Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia. Biodiversitas 21: 3242-3248. Ehrlichia canis is Gram-negative intracellular obligate bacteria that cause ehrl...

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Autores principales:	yustinus oswin primajuni wuhan, Aris Haryanto, Ida Tjahajati
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	ehrlichia canis, glta gene, hematology, molecular characterization Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aad6454c45234da3b3d04484e9f36895
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spelling	oai:doaj.org-article:aad6454c45234da3b3d04484e9f368952021-11-22T00:40:51ZShort Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia1412-033X2085-472210.13057/biodiv/d210746https://doaj.org/article/aad6454c45234da3b3d04484e9f368952020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5954https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wuhan YOP, Haryanto A, Tjahajati I. 2020. Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia. Biodiversitas 21: 3242-3248. Ehrlichia canis is Gram-negative intracellular obligate bacteria that cause ehrlichiosis, a companion vector-borne disease is a potentially fatal disease that attacks dogs. The purpose of this study was to molecular characterize and determine the features of Ehrlichia-infected blood based on the amplification of the gltA gene in Ehrlichia infected dogs from Yogyakarta, Indonesia. Blood samples were collected from 51 dog patients from the Prof. Dr. Soeparwi Animal Hospital, animal clinics, and pet shops based on the anamnesis, clinical sign, and physical examination, followed by microscopic examination, routine hematology, PCR amplification, and DNA sequencing were carried out on the blood samples. Based on positive PCR amplification and blood hematology profile examination ehrlichiosis-positive in dogs showed that thrombocytopenia case was 82.3%, anemia was 70.5%, eosinopenia was 70.5%, neutropenia was 29.4%, monocytopenia was 23%, leukopenia was 17% and lymphopenia was 11.7%. Morulae of Ehrlichia sp.was not found in microscopic examination. Molecularly, detected of E. canis using the gltA gene showed that 34% of samples were positive results. Then 5 of positive Ehrlichia samples were DNA sequenced, they showed a high homology of 100% with Hat Yai isolates (KU765199.1). There was no genetic diversity between E. canis samples in Yogyakarta.yustinus oswin primajuni wuhanAris HaryantoIda TjahajatiMBI & UNS Soloarticleehrlichia canis, glta gene, hematology, molecular characterizationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ehrlichia canis, glta gene, hematology, molecular characterization Biology (General) QH301-705.5
spellingShingle	ehrlichia canis, glta gene, hematology, molecular characterization Biology (General) QH301-705.5 yustinus oswin primajuni wuhan Aris Haryanto Ida Tjahajati Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia
description	Abstract. Wuhan YOP, Haryanto A, Tjahajati I. 2020. Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia. Biodiversitas 21: 3242-3248. Ehrlichia canis is Gram-negative intracellular obligate bacteria that cause ehrlichiosis, a companion vector-borne disease is a potentially fatal disease that attacks dogs. The purpose of this study was to molecular characterize and determine the features of Ehrlichia-infected blood based on the amplification of the gltA gene in Ehrlichia infected dogs from Yogyakarta, Indonesia. Blood samples were collected from 51 dog patients from the Prof. Dr. Soeparwi Animal Hospital, animal clinics, and pet shops based on the anamnesis, clinical sign, and physical examination, followed by microscopic examination, routine hematology, PCR amplification, and DNA sequencing were carried out on the blood samples. Based on positive PCR amplification and blood hematology profile examination ehrlichiosis-positive in dogs showed that thrombocytopenia case was 82.3%, anemia was 70.5%, eosinopenia was 70.5%, neutropenia was 29.4%, monocytopenia was 23%, leukopenia was 17% and lymphopenia was 11.7%. Morulae of Ehrlichia sp.was not found in microscopic examination. Molecularly, detected of E. canis using the gltA gene showed that 34% of samples were positive results. Then 5 of positive Ehrlichia samples were DNA sequenced, they showed a high homology of 100% with Hat Yai isolates (KU765199.1). There was no genetic diversity between E. canis samples in Yogyakarta.
format	article
author	yustinus oswin primajuni wuhan Aris Haryanto Ida Tjahajati
author_facet	yustinus oswin primajuni wuhan Aris Haryanto Ida Tjahajati
author_sort	yustinus oswin primajuni wuhan
title	Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia
title_short	Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia
title_full	Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia
title_fullStr	Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia
title_full_unstemmed	Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia
title_sort	short communication: molecular characterization and blood hematology profile of dogs infected by ehrlichia canis in yogyakarta, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/aad6454c45234da3b3d04484e9f36895
work_keys_str_mv	AT yustinusoswinprimajuniwuhan shortcommunicationmolecularcharacterizationandbloodhematologyprofileofdogsinfectedbyehrlichiacanisinyogyakartaindonesia AT arisharyanto shortcommunicationmolecularcharacterizationandbloodhematologyprofileofdogsinfectedbyehrlichiacanisinyogyakartaindonesia AT idatjahajati shortcommunicationmolecularcharacterizationandbloodhematologyprofileofdogsinfectedbyehrlichiacanisinyogyakartaindonesia
_version_	1718418542837104640

Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia

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