Influence of Culture Conditions on In Vitro Asymbiotic Germination of Anacamptis longicornu and Ophrys panormitana (Orchidaceae)

This study is the first approach to in vitro asymbiotic germination of two species of Sicilian threatened terrestrial orchids, Anacamptis longicornu and Ophrys panormitana. Seeds were collected in the wild and cultured in two different media—Orchimax medium (OM)...

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Autores principales:	Myriam Arcidiacono, Caterina Catalano, Antonio Motisi, Maurizio Sajeva, Francesco Carimi, Angela Carra
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	acclimatization embryonic developmental stages endangered species Mediterranean terrestrial orchids photoperiod Botany QK1-989
Acceso en línea:	https://doaj.org/article/aad8b5d040004c2c909b32098d049e63
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spelling	oai:doaj.org-article:aad8b5d040004c2c909b32098d049e632021-11-25T18:47:32ZInfluence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)10.3390/plants101125432223-7747https://doaj.org/article/aad8b5d040004c2c909b32098d049e632021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2543https://doaj.org/toc/2223-7747This study is the first approach to in vitro asymbiotic germination of two species of Sicilian threatened terrestrial orchids, <i>Anacamptis longicornu</i> and <i>Ophrys panormitana.</i> Seeds were collected in the wild and cultured in two different media—Orchimax medium (OM) and Murashige and Skoog (MS)—and exposed to different photoperiods and temperatures to evaluate the best conditions for the specific stages of development. The germination of <i>A. longicornu</i> was very high on OM (95.5%) and lower on MS medium (21.4%), whereas <i>O. panormitana</i> germinated only on OM medium, with significantly lower percentages (12.0%), compared with <i>A. longicornu</i>. This difference is caused by variation in quality and quantity of nutrients used, primarily by nitrogen source. The results show that temperature and photoperiod widely affect seed germination and development. Although further investigations on asymbiotic and symbiotic germination are needed for the improvement of conservation of Mediterranean terrestrial orchids, our results contribute to the conservation of this group of plants.Myriam ArcidiaconoCaterina CatalanoAntonio MotisiMaurizio SajevaFrancesco CarimiAngela CarraMDPI AGarticleacclimatizationembryonic developmental stagesendangered speciesMediterranean terrestrial orchidsphotoperiodBotanyQK1-989ENPlants, Vol 10, Iss 2543, p 2543 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acclimatization embryonic developmental stages endangered species Mediterranean terrestrial orchids photoperiod Botany QK1-989
spellingShingle	acclimatization embryonic developmental stages endangered species Mediterranean terrestrial orchids photoperiod Botany QK1-989 Myriam Arcidiacono Caterina Catalano Antonio Motisi Maurizio Sajeva Francesco Carimi Angela Carra Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)
description	This study is the first approach to in vitro asymbiotic germination of two species of Sicilian threatened terrestrial orchids, <i>Anacamptis longicornu</i> and <i>Ophrys panormitana.</i> Seeds were collected in the wild and cultured in two different media—Orchimax medium (OM) and Murashige and Skoog (MS)—and exposed to different photoperiods and temperatures to evaluate the best conditions for the specific stages of development. The germination of <i>A. longicornu</i> was very high on OM (95.5%) and lower on MS medium (21.4%), whereas <i>O. panormitana</i> germinated only on OM medium, with significantly lower percentages (12.0%), compared with <i>A. longicornu</i>. This difference is caused by variation in quality and quantity of nutrients used, primarily by nitrogen source. The results show that temperature and photoperiod widely affect seed germination and development. Although further investigations on asymbiotic and symbiotic germination are needed for the improvement of conservation of Mediterranean terrestrial orchids, our results contribute to the conservation of this group of plants.
format	article
author	Myriam Arcidiacono Caterina Catalano Antonio Motisi Maurizio Sajeva Francesco Carimi Angela Carra
author_facet	Myriam Arcidiacono Caterina Catalano Antonio Motisi Maurizio Sajeva Francesco Carimi Angela Carra
author_sort	Myriam Arcidiacono
title	Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)
title_short	Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)
title_full	Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)
title_fullStr	Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)
title_full_unstemmed	Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)
title_sort	influence of culture conditions on in vitro asymbiotic germination of <i>anacamptis longicornu</i> and <i>ophrys panormitana</i> (orchidaceae)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/aad8b5d040004c2c909b32098d049e63
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Influence of Culture Conditions on In Vitro Asymbiotic Germination of <i>Anacamptis longicornu</i> and <i>Ophrys panormitana</i> (Orchidaceae)

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