Propionibacterium acnes-Derived Circulating Immune Complexes in Sarcoidosis Patients

Propionibacterium acnes is a potential etiologic agent of sarcoidosis and a dysregulated immune response to the commensal bacterium is suspected to cause granuloma formation. P. acnes-derived insoluble immune complexes were recently demonstrated in sinus macroph...

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Autores principales:	Keisuke Uchida, Asuka Furukawa, Akiko Yoneyama, Haruhiko Furusawa, Daisuke Kobayashi, Takashi Ito, Kurara Yamamoto, Masaki Sekine, Keiko Miura, Takumi Akashi, Yoshinobu Eishi, Kenichi Ohashi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sarcoidosis <i>Propionibacterium acnes</i> <i>Cutibacterium acnes</i> lipoteichoic acid infectious antibody immune complex Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aadfaac3de554a78a3bcad5fbffef29d
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spelling	oai:doaj.org-article:aadfaac3de554a78a3bcad5fbffef29d2021-11-25T18:24:08Z<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients10.3390/microorganisms91121942076-2607https://doaj.org/article/aadfaac3de554a78a3bcad5fbffef29d2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2194https://doaj.org/toc/2076-2607<i>Propionibacterium acnes</i> is a potential etiologic agent of sarcoidosis and a dysregulated immune response to the commensal bacterium is suspected to cause granuloma formation. <i>P. acnes</i>-derived insoluble immune complexes were recently demonstrated in sinus macrophages of sarcoidosis lymph nodes, suggesting local proliferation of the bacterium in affected organs. In the present study, we developed a method for detecting <i>P. acnes</i>-derived immune complexes in human blood by measuring the concentration of <i>P. acnes</i>-specific lipoteichoic acid (PLTA) detectable after an antigen retrieval pretreatment of plasma samples. Before pretreatment, anti-PLTA antibody was detected and PLTA could not be detected, in all plasma samples from 51 sarcoidosis patients and 35 healthy volunteers. After pretreatment, however, a significant level of PLTA (>105 ng/mL) was detected in 33 (65%) sarcoidosis patients and 5 (14%) control subjects, with 86% specificity and 65% sensitivity for sarcoidosis. In both groups, plasma anti-PLTA antibody titers did not differ between samples with and without detection of PLTA. PLTA levels were abnormally increased (>202 ng/mL) in 21 (41%) sarcoidosis patients. These findings suggest that <i>P. acnes</i>-derived circulating immune complexes present in human blood are abnormally increased in many sarcoidosis patients, presumably due to local proliferation of the bacterium in the affected organs.Keisuke UchidaAsuka FurukawaAkiko YoneyamaHaruhiko FurusawaDaisuke KobayashiTakashi ItoKurara YamamotoMasaki SekineKeiko MiuraTakumi AkashiYoshinobu EishiKenichi OhashiMDPI AGarticlesarcoidosis<i>Propionibacterium acnes</i><i>Cutibacterium acnes</i>lipoteichoic acidinfectious antibodyimmune complexBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2194, p 2194 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sarcoidosis <i>Propionibacterium acnes</i> <i>Cutibacterium acnes</i> lipoteichoic acid infectious antibody immune complex Biology (General) QH301-705.5
spellingShingle	sarcoidosis <i>Propionibacterium acnes</i> <i>Cutibacterium acnes</i> lipoteichoic acid infectious antibody immune complex Biology (General) QH301-705.5 Keisuke Uchida Asuka Furukawa Akiko Yoneyama Haruhiko Furusawa Daisuke Kobayashi Takashi Ito Kurara Yamamoto Masaki Sekine Keiko Miura Takumi Akashi Yoshinobu Eishi Kenichi Ohashi <i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients
description	<i>Propionibacterium acnes</i> is a potential etiologic agent of sarcoidosis and a dysregulated immune response to the commensal bacterium is suspected to cause granuloma formation. <i>P. acnes</i>-derived insoluble immune complexes were recently demonstrated in sinus macrophages of sarcoidosis lymph nodes, suggesting local proliferation of the bacterium in affected organs. In the present study, we developed a method for detecting <i>P. acnes</i>-derived immune complexes in human blood by measuring the concentration of <i>P. acnes</i>-specific lipoteichoic acid (PLTA) detectable after an antigen retrieval pretreatment of plasma samples. Before pretreatment, anti-PLTA antibody was detected and PLTA could not be detected, in all plasma samples from 51 sarcoidosis patients and 35 healthy volunteers. After pretreatment, however, a significant level of PLTA (>105 ng/mL) was detected in 33 (65%) sarcoidosis patients and 5 (14%) control subjects, with 86% specificity and 65% sensitivity for sarcoidosis. In both groups, plasma anti-PLTA antibody titers did not differ between samples with and without detection of PLTA. PLTA levels were abnormally increased (>202 ng/mL) in 21 (41%) sarcoidosis patients. These findings suggest that <i>P. acnes</i>-derived circulating immune complexes present in human blood are abnormally increased in many sarcoidosis patients, presumably due to local proliferation of the bacterium in the affected organs.
format	article
author	Keisuke Uchida Asuka Furukawa Akiko Yoneyama Haruhiko Furusawa Daisuke Kobayashi Takashi Ito Kurara Yamamoto Masaki Sekine Keiko Miura Takumi Akashi Yoshinobu Eishi Kenichi Ohashi
author_facet	Keisuke Uchida Asuka Furukawa Akiko Yoneyama Haruhiko Furusawa Daisuke Kobayashi Takashi Ito Kurara Yamamoto Masaki Sekine Keiko Miura Takumi Akashi Yoshinobu Eishi Kenichi Ohashi
author_sort	Keisuke Uchida
title	<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients
title_short	<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients
title_full	<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients
title_fullStr	<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients
title_full_unstemmed	<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients
title_sort	<i>propionibacterium acnes</i>-derived circulating immune complexes in sarcoidosis patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/aadfaac3de554a78a3bcad5fbffef29d
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<i>Propionibacterium acnes</i>-Derived Circulating Immune Complexes in Sarcoidosis Patients

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