Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress

Abstract The pollination services provided by the honey bee are critical in both natural and agricultural ecosystems. Honey bee colonies in the United States have suffered from an increased rate of die-off in recent years, stemming from a complex set of interacting stresses that remain poorly descri...

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Autores principales:	Samantha R. Shih, Dunay M. Bach, Nicole C. Rondeau, Jessica Sam, Natalie L. Lovinger, Allison J. Lopatkin, Jonathan W. Snow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aae2f7404e724995b64f1b9e615e3f3e
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spelling	oai:doaj.org-article:aae2f7404e724995b64f1b9e615e3f3e2021-11-14T12:21:49ZHoney bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress10.1038/s41598-021-01547-12045-2322https://doaj.org/article/aae2f7404e724995b64f1b9e615e3f3e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01547-1https://doaj.org/toc/2045-2322Abstract The pollination services provided by the honey bee are critical in both natural and agricultural ecosystems. Honey bee colonies in the United States have suffered from an increased rate of die-off in recent years, stemming from a complex set of interacting stresses that remain poorly described. Defining specific common cellular processes and cellular stress responses impacted by multiple stressors represent a key step in understanding these synergies. Proteotoxic stresses negatively impact protein synthesis, folding, and degradation. Diverse proteotoxic stresses induce expression of genes encoding small heat shock proteins (sHSP) of the expanded lethal (2) essential for life (l(2)efl) gene family. In addition to upregulation by the Integrated Stress Response (ISR), the Heat Shock Response (HSR), and the Oxidative Stress Response (OSR), our data provide first evidence that sHSP genes are upregulated by the Unfolded Protein Response (UPR). As these genes appear to be part of a core stress response that could serve as a useful biomarker for cellular stress in honey bees, we designed and tested an RT-LAMP assay to detect increased l(2)efl gene expression in response to heat-stress. While this assay provides a powerful proof of principle, further work will be necessary to link changes in sHSP gene expression to colony-level outcomes, to adapt our preliminary assay into a Point of Care Testing (POCT) assay appropriate for use as a diagnostic tool for use in the field, and to couple assay results to management recommendations.Samantha R. ShihDunay M. BachNicole C. RondeauJessica SamNatalie L. LovingerAllison J. LopatkinJonathan W. SnowNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samantha R. Shih Dunay M. Bach Nicole C. Rondeau Jessica Sam Natalie L. Lovinger Allison J. Lopatkin Jonathan W. Snow Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
description	Abstract The pollination services provided by the honey bee are critical in both natural and agricultural ecosystems. Honey bee colonies in the United States have suffered from an increased rate of die-off in recent years, stemming from a complex set of interacting stresses that remain poorly described. Defining specific common cellular processes and cellular stress responses impacted by multiple stressors represent a key step in understanding these synergies. Proteotoxic stresses negatively impact protein synthesis, folding, and degradation. Diverse proteotoxic stresses induce expression of genes encoding small heat shock proteins (sHSP) of the expanded lethal (2) essential for life (l(2)efl) gene family. In addition to upregulation by the Integrated Stress Response (ISR), the Heat Shock Response (HSR), and the Oxidative Stress Response (OSR), our data provide first evidence that sHSP genes are upregulated by the Unfolded Protein Response (UPR). As these genes appear to be part of a core stress response that could serve as a useful biomarker for cellular stress in honey bees, we designed and tested an RT-LAMP assay to detect increased l(2)efl gene expression in response to heat-stress. While this assay provides a powerful proof of principle, further work will be necessary to link changes in sHSP gene expression to colony-level outcomes, to adapt our preliminary assay into a Point of Care Testing (POCT) assay appropriate for use as a diagnostic tool for use in the field, and to couple assay results to management recommendations.
format	article
author	Samantha R. Shih Dunay M. Bach Nicole C. Rondeau Jessica Sam Natalie L. Lovinger Allison J. Lopatkin Jonathan W. Snow
author_facet	Samantha R. Shih Dunay M. Bach Nicole C. Rondeau Jessica Sam Natalie L. Lovinger Allison J. Lopatkin Jonathan W. Snow
author_sort	Samantha R. Shih
title	Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
title_short	Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
title_full	Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
title_fullStr	Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
title_full_unstemmed	Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
title_sort	honey bee shsp are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aae2f7404e724995b64f1b9e615e3f3e
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Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress

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