Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle

Abstract Cryotherapy is commonly used in the treatment of skeletal muscle injuries. However, the data to support the use of cryotherapy is inconclusive, and the biochemical etiology of cryotherapy in human skeletal muscle remains largely unknown. We therefore sought to determine how a clinically-rel...

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Autores principales:	Dylan C. Sarver, Kristoffer B. Sugg, Nathaniel P. Disser, Elizabeth R. Sibilsky Enselman, Tariq M. Awan, Christopher L. Mendias
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aae5def16cce402b9a6bff686435d621
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spelling	oai:doaj.org-article:aae5def16cce402b9a6bff686435d6212021-12-02T12:32:42ZLocal cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle10.1038/s41598-017-02754-52045-2322https://doaj.org/article/aae5def16cce402b9a6bff686435d6212017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02754-5https://doaj.org/toc/2045-2322Abstract Cryotherapy is commonly used in the treatment of skeletal muscle injuries. However, the data to support the use of cryotherapy is inconclusive, and the biochemical etiology of cryotherapy in human skeletal muscle remains largely unknown. We therefore sought to determine how a clinically-relevant dose of cryotherapy would impact the transcriptome and metabolome of skeletal muscle. Eight healthy male subjects (age 24.7 ± 4.5 years, BMI 22.2 ± 1.6) received a 15 minute bout of local cryotherapy, delivered via ice cup massage over the anterolateral thigh. This resulted in an 85% decrease in skin temperature and a predicted 27% reduction in intramuscular temperature. The contralateral side served as a non-treated control. Two hours after cryotherapy, muscle biopsies were obtained to analyze changes in the transcriptome, metabolome, and activation of p38 MAPK, ERK1/2, Akt, and p70S6K proteins. No changes were detected in the transcriptome between control and cooled muscles. Cryotherapy reduced levels of hexose sugars and hypoxanthine by 1.3%, but no statistically different changes were observed in 60 additional metabolites. Overall, no differences in phosphorylated p38 MAPK, ERK1/2, Akt, and p70S6K were observed. A clinically relevant dose of cryotherapy produced negligible acute biochemical and molecular changes in the skeletal muscle of human subjects.Dylan C. SarverKristoffer B. SuggNathaniel P. DisserElizabeth R. Sibilsky EnselmanTariq M. AwanChristopher L. MendiasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dylan C. Sarver Kristoffer B. Sugg Nathaniel P. Disser Elizabeth R. Sibilsky Enselman Tariq M. Awan Christopher L. Mendias Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
description	Abstract Cryotherapy is commonly used in the treatment of skeletal muscle injuries. However, the data to support the use of cryotherapy is inconclusive, and the biochemical etiology of cryotherapy in human skeletal muscle remains largely unknown. We therefore sought to determine how a clinically-relevant dose of cryotherapy would impact the transcriptome and metabolome of skeletal muscle. Eight healthy male subjects (age 24.7 ± 4.5 years, BMI 22.2 ± 1.6) received a 15 minute bout of local cryotherapy, delivered via ice cup massage over the anterolateral thigh. This resulted in an 85% decrease in skin temperature and a predicted 27% reduction in intramuscular temperature. The contralateral side served as a non-treated control. Two hours after cryotherapy, muscle biopsies were obtained to analyze changes in the transcriptome, metabolome, and activation of p38 MAPK, ERK1/2, Akt, and p70S6K proteins. No changes were detected in the transcriptome between control and cooled muscles. Cryotherapy reduced levels of hexose sugars and hypoxanthine by 1.3%, but no statistically different changes were observed in 60 additional metabolites. Overall, no differences in phosphorylated p38 MAPK, ERK1/2, Akt, and p70S6K were observed. A clinically relevant dose of cryotherapy produced negligible acute biochemical and molecular changes in the skeletal muscle of human subjects.
format	article
author	Dylan C. Sarver Kristoffer B. Sugg Nathaniel P. Disser Elizabeth R. Sibilsky Enselman Tariq M. Awan Christopher L. Mendias
author_facet	Dylan C. Sarver Kristoffer B. Sugg Nathaniel P. Disser Elizabeth R. Sibilsky Enselman Tariq M. Awan Christopher L. Mendias
author_sort	Dylan C. Sarver
title	Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
title_short	Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
title_full	Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
title_fullStr	Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
title_full_unstemmed	Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
title_sort	local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/aae5def16cce402b9a6bff686435d621
work_keys_str_mv	AT dylancsarver localcryotherapyminimallyimpactsthemetabolomeandtranscriptomeofhumanskeletalmuscle AT kristofferbsugg localcryotherapyminimallyimpactsthemetabolomeandtranscriptomeofhumanskeletalmuscle AT nathanielpdisser localcryotherapyminimallyimpactsthemetabolomeandtranscriptomeofhumanskeletalmuscle AT elizabethrsibilskyenselman localcryotherapyminimallyimpactsthemetabolomeandtranscriptomeofhumanskeletalmuscle AT tariqmawan localcryotherapyminimallyimpactsthemetabolomeandtranscriptomeofhumanskeletalmuscle AT christopherlmendias localcryotherapyminimallyimpactsthemetabolomeandtranscriptomeofhumanskeletalmuscle
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Local cryotherapy minimally impacts the metabolome and transcriptome of human skeletal muscle

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