YY1’s role in the Peg3 imprinted domain

Abstract The ICR (Imprinting Control Region) of the Peg3 (Paternally Expressed Gene 3) domain contains an unusual cluster of YY1 binding sites. In the current study, these YY1 binding sites were mutated to characterize the unknown roles in the mouse Peg3 domain. According to the results, paternal an...

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Autores principales:	Hongzhi He, An Ye, Bambarendage P. U. Perera, Joomyeong Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aae8f4d347f64f8a9dcf9bb197d0dc36
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spelling	oai:doaj.org-article:aae8f4d347f64f8a9dcf9bb197d0dc362021-12-02T16:07:46ZYY1’s role in the Peg3 imprinted domain10.1038/s41598-017-06817-52045-2322https://doaj.org/article/aae8f4d347f64f8a9dcf9bb197d0dc362017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06817-5https://doaj.org/toc/2045-2322Abstract The ICR (Imprinting Control Region) of the Peg3 (Paternally Expressed Gene 3) domain contains an unusual cluster of YY1 binding sites. In the current study, these YY1 binding sites were mutated to characterize the unknown roles in the mouse Peg3 domain. According to the results, paternal and maternal transmission of the mutant allele did not cause any major effect on the survival of the pups. In the mutants, the maternal-specific DNA methylation on the ICR was properly established and maintained, causing no major effect on the imprinting of the domain. In contrast, the paternal transmission resulted in changes in the expression levels of several genes: down-regulation of Peg3 and Usp29 and up-regulation of Zim1. These changes were more pronounced during the neonatal stage than during the adult stage. In the case of Peg3 and Zim1, the levels of the observed changes were also different between males and females, suggesting the different degrees of YY1 involvement between two sexes. Overall, the results indicated that YY1 is mainly involved in controlling the transcriptional levels, but not the DNA methylation, of the Peg3 domain.Hongzhi HeAn YeBambarendage P. U. PereraJoomyeong KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongzhi He An Ye Bambarendage P. U. Perera Joomyeong Kim YY1’s role in the Peg3 imprinted domain
description	Abstract The ICR (Imprinting Control Region) of the Peg3 (Paternally Expressed Gene 3) domain contains an unusual cluster of YY1 binding sites. In the current study, these YY1 binding sites were mutated to characterize the unknown roles in the mouse Peg3 domain. According to the results, paternal and maternal transmission of the mutant allele did not cause any major effect on the survival of the pups. In the mutants, the maternal-specific DNA methylation on the ICR was properly established and maintained, causing no major effect on the imprinting of the domain. In contrast, the paternal transmission resulted in changes in the expression levels of several genes: down-regulation of Peg3 and Usp29 and up-regulation of Zim1. These changes were more pronounced during the neonatal stage than during the adult stage. In the case of Peg3 and Zim1, the levels of the observed changes were also different between males and females, suggesting the different degrees of YY1 involvement between two sexes. Overall, the results indicated that YY1 is mainly involved in controlling the transcriptional levels, but not the DNA methylation, of the Peg3 domain.
format	article
author	Hongzhi He An Ye Bambarendage P. U. Perera Joomyeong Kim
author_facet	Hongzhi He An Ye Bambarendage P. U. Perera Joomyeong Kim
author_sort	Hongzhi He
title	YY1’s role in the Peg3 imprinted domain
title_short	YY1’s role in the Peg3 imprinted domain
title_full	YY1’s role in the Peg3 imprinted domain
title_fullStr	YY1’s role in the Peg3 imprinted domain
title_full_unstemmed	YY1’s role in the Peg3 imprinted domain
title_sort	yy1’s role in the peg3 imprinted domain
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/aae8f4d347f64f8a9dcf9bb197d0dc36
work_keys_str_mv	AT hongzhihe yy1sroleinthepeg3imprinteddomain AT anye yy1sroleinthepeg3imprinteddomain AT bambarendagepuperera yy1sroleinthepeg3imprinteddomain AT joomyeongkim yy1sroleinthepeg3imprinteddomain
_version_	1718384736501497856

YY1’s role in the Peg3 imprinted domain

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