Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.

Several regulators are controlling the formation of the photosynthetic apparatus in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. Among the proteins affecting photosynthesis gene expression is the blue light photoreceptor cryptochrome CryB. This study addresses the effect of Cr...

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Autores principales:	Sebastian Frühwirth, Kristin Teich, Gabriele Klug
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aaf30372db21443db8340df060ee69cb
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spelling	oai:doaj.org-article:aaf30372db21443db8340df060ee69cb2021-11-18T07:23:09ZEffects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.1932-620310.1371/journal.pone.0033791https://doaj.org/article/aaf30372db21443db8340df060ee69cb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22496766/?tool=EBIhttps://doaj.org/toc/1932-6203Several regulators are controlling the formation of the photosynthetic apparatus in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. Among the proteins affecting photosynthesis gene expression is the blue light photoreceptor cryptochrome CryB. This study addresses the effect of CryB on global gene expression. The data reveal that CryB does not only influence photosynthesis gene expression but also genes for the non-photosynthetic energy metabolism like citric acid cycle and oxidative phosphorylation. In addition several genes involved in RNA processing and in transcriptional regulation are affected by a cryB deletion. Although CryB was shown to undergo a photocycle it does not only affect gene expression in response to blue light illumination but also in response to singlet oxygen stress conditions. While there is a large overlap in these responses, some CryB-dependent effects are specific for blue-light or photooxidative stress. In addition to protein-coding genes some genes for sRNAs show CryB-dependent expression. These findings give new insight into the function of bacterial cryptochromes and demonstrate for the first time a function in the oxidative stress response.Sebastian FrühwirthKristin TeichGabriele KlugPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e33791 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Frühwirth Kristin Teich Gabriele Klug Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
description	Several regulators are controlling the formation of the photosynthetic apparatus in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. Among the proteins affecting photosynthesis gene expression is the blue light photoreceptor cryptochrome CryB. This study addresses the effect of CryB on global gene expression. The data reveal that CryB does not only influence photosynthesis gene expression but also genes for the non-photosynthetic energy metabolism like citric acid cycle and oxidative phosphorylation. In addition several genes involved in RNA processing and in transcriptional regulation are affected by a cryB deletion. Although CryB was shown to undergo a photocycle it does not only affect gene expression in response to blue light illumination but also in response to singlet oxygen stress conditions. While there is a large overlap in these responses, some CryB-dependent effects are specific for blue-light or photooxidative stress. In addition to protein-coding genes some genes for sRNAs show CryB-dependent expression. These findings give new insight into the function of bacterial cryptochromes and demonstrate for the first time a function in the oxidative stress response.
format	article
author	Sebastian Frühwirth Kristin Teich Gabriele Klug
author_facet	Sebastian Frühwirth Kristin Teich Gabriele Klug
author_sort	Sebastian Frühwirth
title	Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
title_short	Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
title_full	Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
title_fullStr	Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
title_full_unstemmed	Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
title_sort	effects of the cryptochrome cryb from rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/aaf30372db21443db8340df060ee69cb
work_keys_str_mv	AT sebastianfruhwirth effectsofthecryptochromecrybfromrhodobactersphaeroidesonglobalgeneexpressioninthedarkorbluelightorinthepresenceofsingletoxygen AT kristinteich effectsofthecryptochromecrybfromrhodobactersphaeroidesonglobalgeneexpressioninthedarkorbluelightorinthepresenceofsingletoxygen AT gabrieleklug effectsofthecryptochromecrybfromrhodobactersphaeroidesonglobalgeneexpressioninthedarkorbluelightorinthepresenceofsingletoxygen
_version_	1718423555386900480

Effects of the cryptochrome CryB from Rhodobacter sphaeroides on global gene expression in the dark or blue light or in the presence of singlet oxygen.

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