Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis

Abstract In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatm...

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Autores principales:	Christiano Calixto Conceição, Jhenifer Nascimento da Silva, Angélica Arcanjo, Cíntia Lopes Nogueira, Leonardo Araujo de Abreu, Pedro Lagerblad de Oliveira, Katia C. Gondim, Bruno Moraes, Stephanie Serafim de Carvalho, Renato Martins da Silva, Itabajara da Silva Vaz, Luciano Andrade Moreira, Carlos Logullo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aaf52a57e54445829414d706c350608d
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spelling	oai:doaj.org-article:aaf52a57e54445829414d706c350608d2021-12-02T17:18:20ZAedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis10.1038/s41598-021-98738-72045-2322https://doaj.org/article/aaf52a57e54445829414d706c350608d2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98738-7https://doaj.org/toc/2045-2322Abstract In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.Christiano Calixto ConceiçãoJhenifer Nascimento da SilvaAngélica ArcanjoCíntia Lopes NogueiraLeonardo Araujo de AbreuPedro Lagerblad de OliveiraKatia C. GondimBruno MoraesStephanie Serafim de CarvalhoRenato Martins da SilvaItabajara da Silva VazLuciano Andrade MoreiraCarlos LogulloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christiano Calixto Conceição Jhenifer Nascimento da Silva Angélica Arcanjo Cíntia Lopes Nogueira Leonardo Araujo de Abreu Pedro Lagerblad de Oliveira Katia C. Gondim Bruno Moraes Stephanie Serafim de Carvalho Renato Martins da Silva Itabajara da Silva Vaz Luciano Andrade Moreira Carlos Logullo Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
description	Abstract In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.
format	article
author	Christiano Calixto Conceição Jhenifer Nascimento da Silva Angélica Arcanjo Cíntia Lopes Nogueira Leonardo Araujo de Abreu Pedro Lagerblad de Oliveira Katia C. Gondim Bruno Moraes Stephanie Serafim de Carvalho Renato Martins da Silva Itabajara da Silva Vaz Luciano Andrade Moreira Carlos Logullo
author_facet	Christiano Calixto Conceição Jhenifer Nascimento da Silva Angélica Arcanjo Cíntia Lopes Nogueira Leonardo Araujo de Abreu Pedro Lagerblad de Oliveira Katia C. Gondim Bruno Moraes Stephanie Serafim de Carvalho Renato Martins da Silva Itabajara da Silva Vaz Luciano Andrade Moreira Carlos Logullo
author_sort	Christiano Calixto Conceição
title	Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_short	Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_full	Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_fullStr	Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_full_unstemmed	Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_sort	aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-wolbachia symbiosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aaf52a57e54445829414d706c350608d
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Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis

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