Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses

Abstract Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood...

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Autores principales:	Jessie Edson, Justin Brown, William L. Miller, W. David Walter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aaf6f449e0a94231bde1061f6777edd4
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spelling	oai:doaj.org-article:aaf6f449e0a94231bde1061f6777edd42021-12-02T16:58:20ZComparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses10.1038/s41598-021-89390-22045-2322https://doaj.org/article/aaf6f449e0a94231bde1061f6777edd42021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89390-2https://doaj.org/toc/2045-2322Abstract Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood is the most common sample collected in vials or on Whatman Flinders Technology Associates (FTA) cards with short- and long-term storage providing adequate DNA for study objectives. The focus of this study was to determine if biological samples stored on Whatman FTA Elute cards were a reasonable alternative to traditional DNA sample collection, storage, and extraction. Tissue, nasal swabs, and ocular fluid were collected from white-tailed deer (Odocoileus virginianus). Tissue samples and nasal swabs acted as a control to compare extraction and DNA suitability for microsatellite analysis for nasal swabs and ocular fluid extracted from FTA Elute cards. We determined that FTA Elute cards improved the extraction time and storage of samples and that nasal swabs and ocular fluid containing pigmented fluid were reasonable alternatives to traditional tissue DNA extractions.Jessie EdsonJustin BrownWilliam L. MillerW. David WalterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessie Edson Justin Brown William L. Miller W. David Walter Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
description	Abstract Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood is the most common sample collected in vials or on Whatman Flinders Technology Associates (FTA) cards with short- and long-term storage providing adequate DNA for study objectives. The focus of this study was to determine if biological samples stored on Whatman FTA Elute cards were a reasonable alternative to traditional DNA sample collection, storage, and extraction. Tissue, nasal swabs, and ocular fluid were collected from white-tailed deer (Odocoileus virginianus). Tissue samples and nasal swabs acted as a control to compare extraction and DNA suitability for microsatellite analysis for nasal swabs and ocular fluid extracted from FTA Elute cards. We determined that FTA Elute cards improved the extraction time and storage of samples and that nasal swabs and ocular fluid containing pigmented fluid were reasonable alternatives to traditional tissue DNA extractions.
format	article
author	Jessie Edson Justin Brown William L. Miller W. David Walter
author_facet	Jessie Edson Justin Brown William L. Miller W. David Walter
author_sort	Jessie Edson
title	Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
title_short	Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
title_full	Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
title_fullStr	Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
title_full_unstemmed	Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
title_sort	comparison of sample types from white-tailed deer (odocoileus virginianus) for dna extraction and analyses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aaf6f449e0a94231bde1061f6777edd4
work_keys_str_mv	AT jessieedson comparisonofsampletypesfromwhitetaileddeerodocoileusvirginianusfordnaextractionandanalyses AT justinbrown comparisonofsampletypesfromwhitetaileddeerodocoileusvirginianusfordnaextractionandanalyses AT williamlmiller comparisonofsampletypesfromwhitetaileddeerodocoileusvirginianusfordnaextractionandanalyses AT wdavidwalter comparisonofsampletypesfromwhitetaileddeerodocoileusvirginianusfordnaextractionandanalyses
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Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses

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