In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.

Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free...

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Autores principales:	Laura FLÓREZ-SAMPEDRO, Wildeman ZAPATA, Lina P. Zapata, Amanda Ines MEJÍA, Carolina ARBOLEDA, Maria Teresa RUGELES
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2016
Materias:	Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase antiviral activity natural products. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/aafaea099c7a490fbda64ca0f0ed0443
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spelling	oai:doaj.org-article:aafaea099c7a490fbda64ca0f0ed04432021-11-19T04:09:47ZIn vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.0121-40042145-2660https://doaj.org/article/aafaea099c7a490fbda64ca0f0ed04432016-09-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/23524https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Aims: In this study we evaluated the anti-HIV-1 activity of the enzymatic crude (EC) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EC and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for GFP and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The ECs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EC from Lentinus sp. had the best selectivity index (SI: 4.77). Conclusions: These results suggest the potential anti-HIV-1 of the EC for the exploration of an alternative therapy against HIV-1 infection. Laura FLÓREZ-SAMPEDROWildeman ZAPATALina P. ZapataAmanda Ines MEJÍACarolina ARBOLEDAMaria Teresa RUGELESUniversidad de AntioquiaarticleHuman immunodeficiency virus type 1 (HIV-1)GanodermaLentinuslaccaseantiviral activitynatural products.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 23, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase antiviral activity natural products. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase antiviral activity natural products. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Laura FLÓREZ-SAMPEDRO Wildeman ZAPATA Lina P. Zapata Amanda Ines MEJÍA Carolina ARBOLEDA Maria Teresa RUGELES In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
description	Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Aims: In this study we evaluated the anti-HIV-1 activity of the enzymatic crude (EC) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EC and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for GFP and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The ECs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EC from Lentinus sp. had the best selectivity index (SI: 4.77). Conclusions: These results suggest the potential anti-HIV-1 of the EC for the exploration of an alternative therapy against HIV-1 infection.
format	article
author	Laura FLÓREZ-SAMPEDRO Wildeman ZAPATA Lina P. Zapata Amanda Ines MEJÍA Carolina ARBOLEDA Maria Teresa RUGELES
author_facet	Laura FLÓREZ-SAMPEDRO Wildeman ZAPATA Lina P. Zapata Amanda Ines MEJÍA Carolina ARBOLEDA Maria Teresa RUGELES
author_sort	Laura FLÓREZ-SAMPEDRO
title	In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
title_short	In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
title_full	In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
title_fullStr	In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
title_full_unstemmed	In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.
title_sort	in vitro anti-hiv-1 activity of the enzymatic extract enriched with laccase produced by the fungi ganoderma sp. and lentinus sp.
publisher	Universidad de Antioquia
publishDate	2016
url	https://doaj.org/article/aafaea099c7a490fbda64ca0f0ed0443
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In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.

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