The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization
The aim of this study was to evaluate the role of the regulatory small RNA (sRNA) Ern0160 in gastrointestinal tract (GIT) colonization by Enterococcus faecium. For this purpose, four strains of E. faecium were used, Aus0004 (WT), an ern0160-deleted Aus0004 mutant (Δ0160), a trans-complemented Δ0160...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/ab01dc7a34244511acc235600f42a732 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:ab01dc7a34244511acc235600f42a732 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:ab01dc7a34244511acc235600f42a7322021-11-10T06:36:51ZThe Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization1664-302X10.3389/fmicb.2021.757227https://doaj.org/article/ab01dc7a34244511acc235600f42a7322021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.757227/fullhttps://doaj.org/toc/1664-302XThe aim of this study was to evaluate the role of the regulatory small RNA (sRNA) Ern0160 in gastrointestinal tract (GIT) colonization by Enterococcus faecium. For this purpose, four strains of E. faecium were used, Aus0004 (WT), an ern0160-deleted Aus0004 mutant (Δ0160), a trans-complemented Δ0160 strain overexpressing ern0160 (Δ0160_0160), and a strain Δ0160 with an empty pAT29 vector (Δ0160_pAT29). Strains were studied both in vitro and in vivo, alone and in competitive assays. In in vitro experiments, no difference was observed between WT and Δ0160 strains cultured single while Δ0160_0160 strain grew more slowly than Δ0160_pAT29. In competitive assays, the WT strain was predominant compared to the deleted strain Δ0160 at the end of the experiment. Then, in vivo experiments were performed using a GIT colonization mouse model. Several existing models of GIT colonization were compared while a novel one, combining ceftriaxone and amoxicillin, was developed. A GIT colonization was performed with each strain alone, and no significant difference was noticed. By contrast, significant results were obtained with co-colonization experiments. With WT + Δ0160 suspension, a significant advantage for the WT strain was observed from day 5 to the end of the protocol, suggesting the involvement of ern0160 in GIT colonization. With Δ0160_0160 + Δ0160_pAT29 suspension, the strain with the empty vector took the advantage from day 3 to the end of the protocol, suggesting a deleterious effect of ern0160 overexpression. Altogether, these findings demonstrate the potential implication of Ern0160 in GIT colonization of E. faecium. Further investigations are needed for the identification of sRNA target(s) in order to decipher underlying molecular mechanisms.Sophie ReissierKillian Le NeindreKillian Le NeindreValérie BordeauLoren DejoiesLoren DejoiesAudrey Le BotAudrey Le BotBrice FeldenVincent CattoirVincent CattoirMatthieu RevestMatthieu RevestFrontiers Media S.A.articleE. faeciumVREsRNApathogenicityanimal modelgut colonizationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
E. faecium VRE sRNA pathogenicity animal model gut colonization Microbiology QR1-502 |
| spellingShingle |
E. faecium VRE sRNA pathogenicity animal model gut colonization Microbiology QR1-502 Sophie Reissier Killian Le Neindre Killian Le Neindre Valérie Bordeau Loren Dejoies Loren Dejoies Audrey Le Bot Audrey Le Bot Brice Felden Vincent Cattoir Vincent Cattoir Matthieu Revest Matthieu Revest The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization |
| description |
The aim of this study was to evaluate the role of the regulatory small RNA (sRNA) Ern0160 in gastrointestinal tract (GIT) colonization by Enterococcus faecium. For this purpose, four strains of E. faecium were used, Aus0004 (WT), an ern0160-deleted Aus0004 mutant (Δ0160), a trans-complemented Δ0160 strain overexpressing ern0160 (Δ0160_0160), and a strain Δ0160 with an empty pAT29 vector (Δ0160_pAT29). Strains were studied both in vitro and in vivo, alone and in competitive assays. In in vitro experiments, no difference was observed between WT and Δ0160 strains cultured single while Δ0160_0160 strain grew more slowly than Δ0160_pAT29. In competitive assays, the WT strain was predominant compared to the deleted strain Δ0160 at the end of the experiment. Then, in vivo experiments were performed using a GIT colonization mouse model. Several existing models of GIT colonization were compared while a novel one, combining ceftriaxone and amoxicillin, was developed. A GIT colonization was performed with each strain alone, and no significant difference was noticed. By contrast, significant results were obtained with co-colonization experiments. With WT + Δ0160 suspension, a significant advantage for the WT strain was observed from day 5 to the end of the protocol, suggesting the involvement of ern0160 in GIT colonization. With Δ0160_0160 + Δ0160_pAT29 suspension, the strain with the empty vector took the advantage from day 3 to the end of the protocol, suggesting a deleterious effect of ern0160 overexpression. Altogether, these findings demonstrate the potential implication of Ern0160 in GIT colonization of E. faecium. Further investigations are needed for the identification of sRNA target(s) in order to decipher underlying molecular mechanisms. |
| format |
article |
| author |
Sophie Reissier Killian Le Neindre Killian Le Neindre Valérie Bordeau Loren Dejoies Loren Dejoies Audrey Le Bot Audrey Le Bot Brice Felden Vincent Cattoir Vincent Cattoir Matthieu Revest Matthieu Revest |
| author_facet |
Sophie Reissier Killian Le Neindre Killian Le Neindre Valérie Bordeau Loren Dejoies Loren Dejoies Audrey Le Bot Audrey Le Bot Brice Felden Vincent Cattoir Vincent Cattoir Matthieu Revest Matthieu Revest |
| author_sort |
Sophie Reissier |
| title |
The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization |
| title_short |
The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization |
| title_full |
The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization |
| title_fullStr |
The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization |
| title_full_unstemmed |
The Regulatory RNA ern0160 Confers a Potential Selective Advantage to Enterococcus faecium for Intestinal Colonization |
| title_sort |
regulatory rna ern0160 confers a potential selective advantage to enterococcus faecium for intestinal colonization |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/ab01dc7a34244511acc235600f42a732 |
| work_keys_str_mv |
AT sophiereissier theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT killianleneindre theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT killianleneindre theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT valeriebordeau theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT lorendejoies theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT lorendejoies theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT audreylebot theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT audreylebot theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT bricefelden theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT vincentcattoir theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT vincentcattoir theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT matthieurevest theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT matthieurevest theregulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT sophiereissier regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT killianleneindre regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT killianleneindre regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT valeriebordeau regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT lorendejoies regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT lorendejoies regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT audreylebot regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT audreylebot regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT bricefelden regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT vincentcattoir regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT vincentcattoir regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT matthieurevest regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization AT matthieurevest regulatoryrnaern0160confersapotentialselectiveadvantagetoenterococcusfaeciumforintestinalcolonization |
| _version_ |
1718440498579898368 |