Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis

Colon tumor endothelial cells (CTECs) plays substantial roles to induce immune invasion, angiogenesis and metastasis. Thus, identification of the CTECs-derived transcriptomes could be helpful for colon cancer diagnosis and potential therapy. Methods: By analysis of CTECs-derived gene expression...

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Autores principales:	Jie Wang, Md. Nazim Uddin, Rehana Akter, Yun Wu
Formato:	article
Lenguaje:	EN
Publicado:	AIMS Press 2021
Materias:	colon tumor endothelial cells prognostic hub genes immune-inhibitory markers immunosuppressive cells colon tumor microenvironment Biotechnology TP248.13-248.65 Mathematics QA1-939
Acceso en línea:	https://doaj.org/article/ab0421ed17104ffeb4a919af269c5999
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spelling	oai:doaj.org-article:ab0421ed17104ffeb4a919af269c59992021-11-23T01:51:12ZContribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis10.3934/mbe.20213601551-0018https://doaj.org/article/ab0421ed17104ffeb4a919af269c59992021-08-01T00:00:00Zhttps://www.aimspress.com/article/doi/10.3934/mbe.2021360?viewType=HTMLhttps://doaj.org/toc/1551-0018Colon tumor endothelial cells (CTECs) plays substantial roles to induce immune invasion, angiogenesis and metastasis. Thus, identification of the CTECs-derived transcriptomes could be helpful for colon cancer diagnosis and potential therapy. Methods: By analysis of CTECs-derived gene expression profiling dataset, we identified differentially expressed genes (DEGs) between CTECs and colon normal endothelial cells (CNECs). In addition, we identified the significant pathways and protein-protein interaction (PPI) network that was significantly associated with the DEGs. Furthermore, we identified hub genes whose expression was significantly associated with prognosis and immune cell infiltrations in colon cancer. Finally, we identified the significant correlations between the prognostic hub genes and immune-inhibitory markers in colon cancer. Results: We identified 362 DEGs in CTECs relative to the CNECs, including117 up-regulated genes and 245 down-regulated genes in the CTECs. In addition, we identified significantly up-regulated pathways in CTECs that were mainly involved in cancer and immune regulation. Furthermore, we identified hub genes (such as SPARC, COL1A1, COL1A2 and IGFBP3) that are associated with prognosis and immune cells infiltrations in colon cancer. Interestingly, we found that prognosis-associated hub genes (SPARC, COL1A1, COL1A2 and IGFBP3) are positively correlated with immune-inhibitory markers of various immunosuppressive cells, including TAM, M2 macrophage, Tregs and T cell exhaustion. Finally, our findings revealed that prognosis-associated upregulated hub genes are positively correlated with immune checkpoint markers, including PD-L1 and PD-L2 and the immunosuppressive markers including TGFB1 and TGFBR1. Conclusions: The identification of CTECs-specific transcriptomes may provide crucial insights into the colon tumor microenvironment that mediates the development of colon cancer.Jie WangMd. Nazim UddinRehana AkterYun WuAIMS Pressarticlecolon tumor endothelial cellsprognostic hub genesimmune-inhibitory markersimmunosuppressive cellscolon tumor microenvironmentBiotechnologyTP248.13-248.65MathematicsQA1-939ENMathematical Biosciences and Engineering, Vol 18, Iss 6, Pp 7280-7300 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colon tumor endothelial cells prognostic hub genes immune-inhibitory markers immunosuppressive cells colon tumor microenvironment Biotechnology TP248.13-248.65 Mathematics QA1-939
spellingShingle	colon tumor endothelial cells prognostic hub genes immune-inhibitory markers immunosuppressive cells colon tumor microenvironment Biotechnology TP248.13-248.65 Mathematics QA1-939 Jie Wang Md. Nazim Uddin Rehana Akter Yun Wu Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
description	Colon tumor endothelial cells (CTECs) plays substantial roles to induce immune invasion, angiogenesis and metastasis. Thus, identification of the CTECs-derived transcriptomes could be helpful for colon cancer diagnosis and potential therapy. Methods: By analysis of CTECs-derived gene expression profiling dataset, we identified differentially expressed genes (DEGs) between CTECs and colon normal endothelial cells (CNECs). In addition, we identified the significant pathways and protein-protein interaction (PPI) network that was significantly associated with the DEGs. Furthermore, we identified hub genes whose expression was significantly associated with prognosis and immune cell infiltrations in colon cancer. Finally, we identified the significant correlations between the prognostic hub genes and immune-inhibitory markers in colon cancer. Results: We identified 362 DEGs in CTECs relative to the CNECs, including117 up-regulated genes and 245 down-regulated genes in the CTECs. In addition, we identified significantly up-regulated pathways in CTECs that were mainly involved in cancer and immune regulation. Furthermore, we identified hub genes (such as SPARC, COL1A1, COL1A2 and IGFBP3) that are associated with prognosis and immune cells infiltrations in colon cancer. Interestingly, we found that prognosis-associated hub genes (SPARC, COL1A1, COL1A2 and IGFBP3) are positively correlated with immune-inhibitory markers of various immunosuppressive cells, including TAM, M2 macrophage, Tregs and T cell exhaustion. Finally, our findings revealed that prognosis-associated upregulated hub genes are positively correlated with immune checkpoint markers, including PD-L1 and PD-L2 and the immunosuppressive markers including TGFB1 and TGFBR1. Conclusions: The identification of CTECs-specific transcriptomes may provide crucial insights into the colon tumor microenvironment that mediates the development of colon cancer.
format	article
author	Jie Wang Md. Nazim Uddin Rehana Akter Yun Wu
author_facet	Jie Wang Md. Nazim Uddin Rehana Akter Yun Wu
author_sort	Jie Wang
title	Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
title_short	Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
title_full	Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
title_fullStr	Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
title_full_unstemmed	Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
title_sort	contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis
publisher	AIMS Press
publishDate	2021
url	https://doaj.org/article/ab0421ed17104ffeb4a919af269c5999
work_keys_str_mv	AT jiewang contributionofendothelialcellderivedtranscriptomestothecoloncancerbasedonbioinformaticsanalysis AT mdnazimuddin contributionofendothelialcellderivedtranscriptomestothecoloncancerbasedonbioinformaticsanalysis AT rehanaakter contributionofendothelialcellderivedtranscriptomestothecoloncancerbasedonbioinformaticsanalysis AT yunwu contributionofendothelialcellderivedtranscriptomestothecoloncancerbasedonbioinformaticsanalysis
_version_	1718417394538381312

Contribution of endothelial cell-derived transcriptomes to the colon cancer based on bioinformatics analysis

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