Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells

Lih-Rou Rau,1 Wan-Yu Huang,1 Jiunn-Woei Liaw,2–5 Shiao-Wen Tsai1,3,6 1Graduate Institute of Biochemical and Biomedical Engineering, 2Department of Mechanical Engineering, 3Center for Biomedical Engineering, Chang Gung University, 4Institute for Radiological Research, Chang Gung Memorial H...

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Autores principales:	Rau LR, Huang WY, Liaw JW, Tsai SW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	pulsed laser photothermal gold nanoparticles osteoblast apoptosis mineralization Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ab0920004c1f4433add49059534dd185
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spelling	oai:doaj.org-article:ab0920004c1f4433add49059534dd1852021-12-02T07:13:45ZPhotothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells1178-2013https://doaj.org/article/ab0920004c1f4433add49059534dd1852016-07-01T00:00:00Zhttps://www.dovepress.com/photothermal-effects-of-laser-activated-surface-plasmonic-gold-nanopar-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lih-Rou Rau,1 Wan-Yu Huang,1 Jiunn-Woei Liaw,2–5 Shiao-Wen Tsai1,3,6 1Graduate Institute of Biochemical and Biomedical Engineering, 2Department of Mechanical Engineering, 3Center for Biomedical Engineering, Chang Gung University, 4Institute for Radiological Research, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan, 5Center for Advanced Molecular Imaging and Translation, 6Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan, Republic of China Abstract: The specific properties of gold nanoparticles (AuNPs) make them a novel class of photothermal agents that can induce cancer cell damage and even death through the conversion of optical energy to thermal energy. Most relevant studies have focused on increasing the precision of cell targeting, improving the efficacy of energy transfer, and exploring additional functions. Nevertheless, most cells can uptake nanosized particles through nonspecific endocytosis; therefore, before hyperthermia via AuNPs can be applied for clinical use, it is important to understand the adverse optical–thermal effects of AuNPs on nontargeted cells. However, few studies have investigated the thermal effects induced by pulsed laser-activated AuNPs on nearby healthy cells due to nonspecific treatment. The aim of this study is to evaluate the photothermal effects induced by AuNPs plus a pulsed laser on MG63, an osteoblast-like cell line, specifically examining the effects on cell morphology, viability, death program, and differentiation. The cells were treated with media containing 50 nm AuNPs at a concentration of 5 ppm for 1 hour. Cultured cells were then exposed to irradiation at 60 mW/cm2 and 80 mW/cm2 by a Nd:YAG laser (532 nm wavelength). We observed that the cytoskeletons of MG63 cells treated with bare AuNPs followed by pulsed laser irradiation were damaged, and these cells had few bubbles on the cell membrane compared with those that were not treated (control) or were treated with AuNPs or the laser alone. There were no significant differences between the AuNPs plus laser treatment group and the other groups in terms of cell viability, death program analysis results, or alkaline phosphatase and calcium accumulation during culture for up to 21 days. However, the calcium deposit areas in the cells treated with AuNPs plus laser were larger than those in other groups during the early culture period. Keywords: pulsed laser, photothermal, gold nanoparticles, osteoblast, apoptosis, mineralizationRau LRHuang WYLiaw JWTsai SWDove Medical Pressarticlepulsed laserphotothermalgold nanoparticlesosteoblastapoptosismineralizationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3461-3473 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	pulsed laser photothermal gold nanoparticles osteoblast apoptosis mineralization Medicine (General) R5-920
spellingShingle	pulsed laser photothermal gold nanoparticles osteoblast apoptosis mineralization Medicine (General) R5-920 Rau LR Huang WY Liaw JW Tsai SW Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
description	Lih-Rou Rau,1 Wan-Yu Huang,1 Jiunn-Woei Liaw,2–5 Shiao-Wen Tsai1,3,6 1Graduate Institute of Biochemical and Biomedical Engineering, 2Department of Mechanical Engineering, 3Center for Biomedical Engineering, Chang Gung University, 4Institute for Radiological Research, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan, 5Center for Advanced Molecular Imaging and Translation, 6Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan, Republic of China Abstract: The specific properties of gold nanoparticles (AuNPs) make them a novel class of photothermal agents that can induce cancer cell damage and even death through the conversion of optical energy to thermal energy. Most relevant studies have focused on increasing the precision of cell targeting, improving the efficacy of energy transfer, and exploring additional functions. Nevertheless, most cells can uptake nanosized particles through nonspecific endocytosis; therefore, before hyperthermia via AuNPs can be applied for clinical use, it is important to understand the adverse optical–thermal effects of AuNPs on nontargeted cells. However, few studies have investigated the thermal effects induced by pulsed laser-activated AuNPs on nearby healthy cells due to nonspecific treatment. The aim of this study is to evaluate the photothermal effects induced by AuNPs plus a pulsed laser on MG63, an osteoblast-like cell line, specifically examining the effects on cell morphology, viability, death program, and differentiation. The cells were treated with media containing 50 nm AuNPs at a concentration of 5 ppm for 1 hour. Cultured cells were then exposed to irradiation at 60 mW/cm2 and 80 mW/cm2 by a Nd:YAG laser (532 nm wavelength). We observed that the cytoskeletons of MG63 cells treated with bare AuNPs followed by pulsed laser irradiation were damaged, and these cells had few bubbles on the cell membrane compared with those that were not treated (control) or were treated with AuNPs or the laser alone. There were no significant differences between the AuNPs plus laser treatment group and the other groups in terms of cell viability, death program analysis results, or alkaline phosphatase and calcium accumulation during culture for up to 21 days. However, the calcium deposit areas in the cells treated with AuNPs plus laser were larger than those in other groups during the early culture period. Keywords: pulsed laser, photothermal, gold nanoparticles, osteoblast, apoptosis, mineralization
format	article
author	Rau LR Huang WY Liaw JW Tsai SW
author_facet	Rau LR Huang WY Liaw JW Tsai SW
author_sort	Rau LR
title	Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
title_short	Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
title_full	Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
title_fullStr	Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
title_full_unstemmed	Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
title_sort	photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/ab0920004c1f4433add49059534dd185
work_keys_str_mv	AT raulr photothermaleffectsoflaseractivatedsurfaceplasmonicgoldnanoparticlesontheapoptosisandosteogenesisofosteoblastlikecells AT huangwy photothermaleffectsoflaseractivatedsurfaceplasmonicgoldnanoparticlesontheapoptosisandosteogenesisofosteoblastlikecells AT liawjw photothermaleffectsoflaseractivatedsurfaceplasmonicgoldnanoparticlesontheapoptosisandosteogenesisofosteoblastlikecells AT tsaisw photothermaleffectsoflaseractivatedsurfaceplasmonicgoldnanoparticlesontheapoptosisandosteogenesisofosteoblastlikecells
_version_	1718399532230770688

Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells

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