Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturall...

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Autores principales:	Kunli Zhang, Pinpin Chu, Shuai Song, Dongxia Yang, Zhibiao Bian, Yan Li, Hongchao Gou, Zhiyong Jiang, Rujian Cai, Chunling Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Haemophilus parasuis outer membrane vesicles purification proteome analysis inflammatory response Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/ab112a9b631c4b4d857f3c178494f140
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spelling	oai:doaj.org-article:ab112a9b631c4b4d857f3c178494f1402021-12-01T08:01:33ZProteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis2297-176910.3389/fvets.2021.756764https://doaj.org/article/ab112a9b631c4b4d857f3c178494f1402021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.756764/fullhttps://doaj.org/toc/2297-1769Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection.Kunli ZhangKunli ZhangPinpin ChuPinpin ChuShuai SongShuai SongDongxia YangDongxia YangZhibiao BianZhibiao BianYan LiYan LiHongchao GouHongchao GouZhiyong JiangZhiyong JiangRujian CaiRujian CaiChunling LiChunling LiFrontiers Media S.A.articleHaemophilus parasuisouter membrane vesiclespurificationproteome analysisinflammatory responseVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Haemophilus parasuis outer membrane vesicles purification proteome analysis inflammatory response Veterinary medicine SF600-1100
spellingShingle	Haemophilus parasuis outer membrane vesicles purification proteome analysis inflammatory response Veterinary medicine SF600-1100 Kunli Zhang Kunli Zhang Pinpin Chu Pinpin Chu Shuai Song Shuai Song Dongxia Yang Dongxia Yang Zhibiao Bian Zhibiao Bian Yan Li Yan Li Hongchao Gou Hongchao Gou Zhiyong Jiang Zhiyong Jiang Rujian Cai Rujian Cai Chunling Li Chunling Li Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
description	Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection.
format	article
author	Kunli Zhang Kunli Zhang Pinpin Chu Pinpin Chu Shuai Song Shuai Song Dongxia Yang Dongxia Yang Zhibiao Bian Zhibiao Bian Yan Li Yan Li Hongchao Gou Hongchao Gou Zhiyong Jiang Zhiyong Jiang Rujian Cai Rujian Cai Chunling Li Chunling Li
author_facet	Kunli Zhang Kunli Zhang Pinpin Chu Pinpin Chu Shuai Song Shuai Song Dongxia Yang Dongxia Yang Zhibiao Bian Zhibiao Bian Yan Li Yan Li Hongchao Gou Hongchao Gou Zhiyong Jiang Zhiyong Jiang Rujian Cai Rujian Cai Chunling Li Chunling Li
author_sort	Kunli Zhang
title	Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
title_short	Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
title_full	Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
title_fullStr	Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
title_full_unstemmed	Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
title_sort	proteome analysis of outer membrane vesicles from a highly virulent strain of haemophilus parasuis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ab112a9b631c4b4d857f3c178494f140
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Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

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