Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat

Abstract Antibiotic-resistant Escherichia coli (E. coli) are common in retail poultry products. In this study, we aimed to isolate and characterize multidrug resistant (MDR) E. coli in raw chicken meat samples collected from poultry shops in Sylhet division, Bangladesh, as well as to determine corre...

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Autores principales:	Md. Masudur Rahman, Asmaul Husna, Hatem A. Elshabrawy, Jahangir Alam, Nurjahan Yasmin Runa, A. T. M. Badruzzaman, Nahid Arjuman Banu, Mohammad Al Mamun, Bashudeb Paul, Shobhan Das, Md. Mahfujur Rahman, A. T. M. Mahbub-E-Elahi, Ahmed S. Khairalla, Hossam M. Ashour
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab19a1318aaf428aae6e545409b85bc6
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spelling	oai:doaj.org-article:ab19a1318aaf428aae6e545409b85bc62021-12-02T13:58:14ZIsolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat10.1038/s41598-020-78367-22045-2322https://doaj.org/article/ab19a1318aaf428aae6e545409b85bc62020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78367-2https://doaj.org/toc/2045-2322Abstract Antibiotic-resistant Escherichia coli (E. coli) are common in retail poultry products. In this study, we aimed to isolate and characterize multidrug resistant (MDR) E. coli in raw chicken meat samples collected from poultry shops in Sylhet division, Bangladesh, as well as to determine correlation between resistance phenotype and genotype. A total of 600 chicken meat swabs (divided equally between broiler and layer farms, n = 300 each) were collected and the isolates identified as E. coli (n = 381) were selected. Disc diffusion antimicrobial susceptibility assay showed resistance of these isolates to ampicillin, erythromycin, tetracycline, streptomycin, trimethoprim-sulfamethoxazole, chloramphenicol, and gentamicin. Polymerase chain reaction (PCR) identified several antibiotic resistance genes (ARGs) in our isolates. Among these ARGs, the prevalence of tetA (for tetracycline) was the highest (72.58%) in broiler chicken isolates, followed by sul1 (for sulfonamide; 44.16%), aadA1 (for streptomycin; 33.50%), ereA (for erythromycin; 27.41%), aac-3-IV (for gentamicin; 25.38%), and the two genes cmlA (24.87%) and catA1 (8.63%) for chloramphenicol. On the other hand, the respective prevalence in layer chicken isolates were 82.06%, 47.83%, 35.87%, 35.33%, 23.91%, 19.02%, and 5.43%. Furthermore, 49.23% of the isolates from broiler chicken were MDR, with the presence of multiple antibiotic resistance genes, including 3 (40.11%) and 4 (9.13%) genes. On the other hand, 51.09% of layer chicken E. coli isolates were MDR, with 3, 4 or 5 ARGs detected in 36.41%, 14.13%, and 0.54% of the isolates, respectively. We also found that 12.8% of broiler chicken E. coli isolates and 7.61% of layer chicken isolates carried genes coding for extended-spectrum SHV beta-lactamases. Lastly, we report the presence of the AmpC beta-lactamase producing gene (CITM) in 4.56% and 3.26% of broiler and layer chicken E. coli isolates, respectively. We found significant correlations between most of the antimicrobial resistant phenotypes and genotypes observed among the investigated E. coli isolates. Our findings highlight the need for the prudent use of antimicrobials in chickens to minimize the development of antibiotic-resistant bacterial strains.Md. Masudur RahmanAsmaul HusnaHatem A. ElshabrawyJahangir AlamNurjahan Yasmin RunaA. T. M. BadruzzamanNahid Arjuman BanuMohammad Al MamunBashudeb PaulShobhan DasMd. Mahfujur RahmanA. T. M. Mahbub-E-ElahiAhmed S. KhairallaHossam M. AshourNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Md. Masudur Rahman Asmaul Husna Hatem A. Elshabrawy Jahangir Alam Nurjahan Yasmin Runa A. T. M. Badruzzaman Nahid Arjuman Banu Mohammad Al Mamun Bashudeb Paul Shobhan Das Md. Mahfujur Rahman A. T. M. Mahbub-E-Elahi Ahmed S. Khairalla Hossam M. Ashour Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
description	Abstract Antibiotic-resistant Escherichia coli (E. coli) are common in retail poultry products. In this study, we aimed to isolate and characterize multidrug resistant (MDR) E. coli in raw chicken meat samples collected from poultry shops in Sylhet division, Bangladesh, as well as to determine correlation between resistance phenotype and genotype. A total of 600 chicken meat swabs (divided equally between broiler and layer farms, n = 300 each) were collected and the isolates identified as E. coli (n = 381) were selected. Disc diffusion antimicrobial susceptibility assay showed resistance of these isolates to ampicillin, erythromycin, tetracycline, streptomycin, trimethoprim-sulfamethoxazole, chloramphenicol, and gentamicin. Polymerase chain reaction (PCR) identified several antibiotic resistance genes (ARGs) in our isolates. Among these ARGs, the prevalence of tetA (for tetracycline) was the highest (72.58%) in broiler chicken isolates, followed by sul1 (for sulfonamide; 44.16%), aadA1 (for streptomycin; 33.50%), ereA (for erythromycin; 27.41%), aac-3-IV (for gentamicin; 25.38%), and the two genes cmlA (24.87%) and catA1 (8.63%) for chloramphenicol. On the other hand, the respective prevalence in layer chicken isolates were 82.06%, 47.83%, 35.87%, 35.33%, 23.91%, 19.02%, and 5.43%. Furthermore, 49.23% of the isolates from broiler chicken were MDR, with the presence of multiple antibiotic resistance genes, including 3 (40.11%) and 4 (9.13%) genes. On the other hand, 51.09% of layer chicken E. coli isolates were MDR, with 3, 4 or 5 ARGs detected in 36.41%, 14.13%, and 0.54% of the isolates, respectively. We also found that 12.8% of broiler chicken E. coli isolates and 7.61% of layer chicken isolates carried genes coding for extended-spectrum SHV beta-lactamases. Lastly, we report the presence of the AmpC beta-lactamase producing gene (CITM) in 4.56% and 3.26% of broiler and layer chicken E. coli isolates, respectively. We found significant correlations between most of the antimicrobial resistant phenotypes and genotypes observed among the investigated E. coli isolates. Our findings highlight the need for the prudent use of antimicrobials in chickens to minimize the development of antibiotic-resistant bacterial strains.
format	article
author	Md. Masudur Rahman Asmaul Husna Hatem A. Elshabrawy Jahangir Alam Nurjahan Yasmin Runa A. T. M. Badruzzaman Nahid Arjuman Banu Mohammad Al Mamun Bashudeb Paul Shobhan Das Md. Mahfujur Rahman A. T. M. Mahbub-E-Elahi Ahmed S. Khairalla Hossam M. Ashour
author_facet	Md. Masudur Rahman Asmaul Husna Hatem A. Elshabrawy Jahangir Alam Nurjahan Yasmin Runa A. T. M. Badruzzaman Nahid Arjuman Banu Mohammad Al Mamun Bashudeb Paul Shobhan Das Md. Mahfujur Rahman A. T. M. Mahbub-E-Elahi Ahmed S. Khairalla Hossam M. Ashour
author_sort	Md. Masudur Rahman
title	Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
title_short	Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
title_full	Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
title_fullStr	Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
title_full_unstemmed	Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
title_sort	isolation and molecular characterization of multidrug-resistant escherichia coli from chicken meat
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ab19a1318aaf428aae6e545409b85bc6
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Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat

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