Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers

Red ginseng (RG) has been extensively utilized in Asian countries due to its pharmacological effects. For the quality evaluation of RG, small molecules, such as ginsenosides, have been widely considered as candidates of its quality markers (Q-markers), and various analytical techniques have been dev...

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Autores principales:	Qian Cheng, Shuhuan Peng, Fangyi Li, Pengdi Cui, Chunxia Zhao, Xiaohui Yan, Tongchuan Suo, Chunhua Wang, Yongzhi He, Zheng Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	compositional monosaccharides HPLC–ELSD/PDA combined with HPSEC–MALLS–RID molecular weight quality markers (Q-marker) red ginseng polysaccharides Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ab20326e5c784d939ed9ed6b325eb3c5
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spelling	oai:doaj.org-article:ab20326e5c784d939ed9ed6b325eb3c52021-11-25T18:59:17ZQuality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers10.3390/separations81101982297-8739https://doaj.org/article/ab20326e5c784d939ed9ed6b325eb3c52021-10-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/198https://doaj.org/toc/2297-8739Red ginseng (RG) has been extensively utilized in Asian countries due to its pharmacological effects. For the quality evaluation of RG, small molecules, such as ginsenosides, have been widely considered as candidates of its quality markers (Q-markers), and various analytical techniques have been developed in order to identify these compounds. However, despite the efforts to analyze the hydrophobic constituents, it is worth pointing out that about 60% of the mass of RG is made of carbohydrates, including mono-, oligo- and polysaccharides. Consequently, the quality differentiation and identification of RG from the perspective of sugar-markers should be focused. High performance liquid chromatography and evaporative light scattering detector (HPLC–ELSD) method for the determination of disaccharides in RG was established. Furthermore, high performance size exclusion chromatography–multi-angle laser light scattering–refractive index detector (HPSEC–MALLS–RID) for the determination of molecular weight and high performance liquid chromatography photodiode array (HPLC–PDA) for the determination of compositional monosaccharides in RG polysaccharides were also established. HPLC–ELSD/PDA combined with HPSEC–MALLS–RID could be used to determine the contents of disaccharides, molecular weights, and compositional monosaccharides of RG polysaccharides, which could be used for quality control, and this is a new view on the sugar marker to quality differentiation of various origins of RG.Qian ChengShuhuan PengFangyi LiPengdi CuiChunxia ZhaoXiaohui YanTongchuan SuoChunhua WangYongzhi HeZheng LiMDPI AGarticlecompositional monosaccharidesHPLC–ELSD/PDA combined with HPSEC–MALLS–RIDmolecular weightquality markers (Q-marker)red ginseng polysaccharidesPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 198, p 198 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	compositional monosaccharides HPLC–ELSD/PDA combined with HPSEC–MALLS–RID molecular weight quality markers (Q-marker) red ginseng polysaccharides Physics QC1-999 Chemistry QD1-999
spellingShingle	compositional monosaccharides HPLC–ELSD/PDA combined with HPSEC–MALLS–RID molecular weight quality markers (Q-marker) red ginseng polysaccharides Physics QC1-999 Chemistry QD1-999 Qian Cheng Shuhuan Peng Fangyi Li Pengdi Cui Chunxia Zhao Xiaohui Yan Tongchuan Suo Chunhua Wang Yongzhi He Zheng Li Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers
description	Red ginseng (RG) has been extensively utilized in Asian countries due to its pharmacological effects. For the quality evaluation of RG, small molecules, such as ginsenosides, have been widely considered as candidates of its quality markers (Q-markers), and various analytical techniques have been developed in order to identify these compounds. However, despite the efforts to analyze the hydrophobic constituents, it is worth pointing out that about 60% of the mass of RG is made of carbohydrates, including mono-, oligo- and polysaccharides. Consequently, the quality differentiation and identification of RG from the perspective of sugar-markers should be focused. High performance liquid chromatography and evaporative light scattering detector (HPLC–ELSD) method for the determination of disaccharides in RG was established. Furthermore, high performance size exclusion chromatography–multi-angle laser light scattering–refractive index detector (HPSEC–MALLS–RID) for the determination of molecular weight and high performance liquid chromatography photodiode array (HPLC–PDA) for the determination of compositional monosaccharides in RG polysaccharides were also established. HPLC–ELSD/PDA combined with HPSEC–MALLS–RID could be used to determine the contents of disaccharides, molecular weights, and compositional monosaccharides of RG polysaccharides, which could be used for quality control, and this is a new view on the sugar marker to quality differentiation of various origins of RG.
format	article
author	Qian Cheng Shuhuan Peng Fangyi Li Pengdi Cui Chunxia Zhao Xiaohui Yan Tongchuan Suo Chunhua Wang Yongzhi He Zheng Li
author_facet	Qian Cheng Shuhuan Peng Fangyi Li Pengdi Cui Chunxia Zhao Xiaohui Yan Tongchuan Suo Chunhua Wang Yongzhi He Zheng Li
author_sort	Qian Cheng
title	Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers
title_short	Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers
title_full	Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers
title_fullStr	Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers
title_full_unstemmed	Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers
title_sort	quality distinguish of red ginseng from different origins by hplc–elsd/pda combined with hpsec–malls–rid, focus on the sugar-markers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ab20326e5c784d939ed9ed6b325eb3c5
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Quality Distinguish of Red Ginseng from Different Origins by HPLC–ELSD/PDA Combined with HPSEC–MALLS–RID, Focus on the Sugar-Markers

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