Versatile selective evolutionary pressure using synthetic defect in universal metabolism

Rational design of enzymes with new or improved properties is rarely straightforward, and artificial selection pressure approaches that link an improvement in the target to cell growth are an alternative. Here, the authors show that diverse enzymes sharing the ubiquitous cofactor NAD(P)+ can substit...

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Autores principales:	Lara Sellés Vidal, James W. Murray, John T. Heap
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ab219fa13e604f7d8d906754f709a9a0
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id	oai:doaj.org-article:ab219fa13e604f7d8d906754f709a9a0
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spelling	oai:doaj.org-article:ab219fa13e604f7d8d906754f709a9a02021-11-28T12:31:33ZVersatile selective evolutionary pressure using synthetic defect in universal metabolism10.1038/s41467-021-27266-92041-1723https://doaj.org/article/ab219fa13e604f7d8d906754f709a9a02021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27266-9https://doaj.org/toc/2041-1723Rational design of enzymes with new or improved properties is rarely straightforward, and artificial selection pressure approaches that link an improvement in the target to cell growth are an alternative. Here, the authors show that diverse enzymes sharing the ubiquitous cofactor NAD(P)+ can substitute for defective NAD+ regeneration, representing a very broadly-applicable artificial selection.Lara Sellés VidalJames W. MurrayJohn T. HeapNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lara Sellés Vidal James W. Murray John T. Heap Versatile selective evolutionary pressure using synthetic defect in universal metabolism
description	Rational design of enzymes with new or improved properties is rarely straightforward, and artificial selection pressure approaches that link an improvement in the target to cell growth are an alternative. Here, the authors show that diverse enzymes sharing the ubiquitous cofactor NAD(P)+ can substitute for defective NAD+ regeneration, representing a very broadly-applicable artificial selection.
format	article
author	Lara Sellés Vidal James W. Murray John T. Heap
author_facet	Lara Sellés Vidal James W. Murray John T. Heap
author_sort	Lara Sellés Vidal
title	Versatile selective evolutionary pressure using synthetic defect in universal metabolism
title_short	Versatile selective evolutionary pressure using synthetic defect in universal metabolism
title_full	Versatile selective evolutionary pressure using synthetic defect in universal metabolism
title_fullStr	Versatile selective evolutionary pressure using synthetic defect in universal metabolism
title_full_unstemmed	Versatile selective evolutionary pressure using synthetic defect in universal metabolism
title_sort	versatile selective evolutionary pressure using synthetic defect in universal metabolism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ab219fa13e604f7d8d906754f709a9a0
work_keys_str_mv	AT larasellesvidal versatileselectiveevolutionarypressureusingsyntheticdefectinuniversalmetabolism AT jameswmurray versatileselectiveevolutionarypressureusingsyntheticdefectinuniversalmetabolism AT johntheap versatileselectiveevolutionarypressureusingsyntheticdefectinuniversalmetabolism
_version_	1718407911130005504

Versatile selective evolutionary pressure using synthetic defect in universal metabolism

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