Versatile selective evolutionary pressure using synthetic defect in universal metabolism
Rational design of enzymes with new or improved properties is rarely straightforward, and artificial selection pressure approaches that link an improvement in the target to cell growth are an alternative. Here, the authors show that diverse enzymes sharing the ubiquitous cofactor NAD(P)+ can substit...
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Autores principales: | Lara Sellés Vidal, James W. Murray, John T. Heap |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/ab219fa13e604f7d8d906754f709a9a0 |
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