Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans

Abstract Long non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. In this study, we used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. Among th...

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Autores principales:	Lingmei Sun, Dan Li, Yujie Yuan, Dayong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab24b211377d4076ac99de8804e6c720
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spelling	oai:doaj.org-article:ab24b211377d4076ac99de8804e6c7202021-12-02T15:23:47ZIntestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans10.1038/s41598-021-81619-42045-2322https://doaj.org/article/ab24b211377d4076ac99de8804e6c7202021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81619-4https://doaj.org/toc/2045-2322Abstract Long non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. In this study, we used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. Among the intestinal lncRNAs, linc-2, linc-46, linc-61, and linc-78 were increased by simulated microgravity treatment, and linc-13, linc-14, linc-50, and linc-125 were decreased by simulated microgravity treatment. Among these 8 intestinal lncRNAs, RNAi knockdown of linc-2 or linc-61 induced a susceptibility to toxicity of simulated microgravity, whereas RNAi knockdown of linc-13, linc-14, or linc-50 induced a resistance to toxicity of simulated microgravity. In simulated microgravity treated nematodes, linc-50 potentially binds to three transcriptional factors (DAF-16, SKN-1, and HLH-30). RNAi knockdown of daf-16, skn-1, or hlh-30 could suppress resistance of linc-50(RNAi) nematodes to the toxicity of simulated microgravity. Therefore, our results provide an important basis for intestinal lncRNAs, such as the linc-50, in regulating the response to simulated microgravity in nematodes.Lingmei SunDan LiYujie YuanDayong WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lingmei Sun Dan Li Yujie Yuan Dayong Wang Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
description	Abstract Long non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. In this study, we used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. Among the intestinal lncRNAs, linc-2, linc-46, linc-61, and linc-78 were increased by simulated microgravity treatment, and linc-13, linc-14, linc-50, and linc-125 were decreased by simulated microgravity treatment. Among these 8 intestinal lncRNAs, RNAi knockdown of linc-2 or linc-61 induced a susceptibility to toxicity of simulated microgravity, whereas RNAi knockdown of linc-13, linc-14, or linc-50 induced a resistance to toxicity of simulated microgravity. In simulated microgravity treated nematodes, linc-50 potentially binds to three transcriptional factors (DAF-16, SKN-1, and HLH-30). RNAi knockdown of daf-16, skn-1, or hlh-30 could suppress resistance of linc-50(RNAi) nematodes to the toxicity of simulated microgravity. Therefore, our results provide an important basis for intestinal lncRNAs, such as the linc-50, in regulating the response to simulated microgravity in nematodes.
format	article
author	Lingmei Sun Dan Li Yujie Yuan Dayong Wang
author_facet	Lingmei Sun Dan Li Yujie Yuan Dayong Wang
author_sort	Lingmei Sun
title	Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
title_short	Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
title_full	Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
title_fullStr	Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
title_full_unstemmed	Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
title_sort	intestinal long non-coding rnas in response to simulated microgravity stress in caenorhabditis elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ab24b211377d4076ac99de8804e6c720
work_keys_str_mv	AT lingmeisun intestinallongnoncodingrnasinresponsetosimulatedmicrogravitystressincaenorhabditiselegans AT danli intestinallongnoncodingrnasinresponsetosimulatedmicrogravitystressincaenorhabditiselegans AT yujieyuan intestinallongnoncodingrnasinresponsetosimulatedmicrogravitystressincaenorhabditiselegans AT dayongwang intestinallongnoncodingrnasinresponsetosimulatedmicrogravitystressincaenorhabditiselegans
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Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans

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