Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.

Maize (Zea mays) is the most widely grown crop species in the world and a classical model organism for plant research. The completion of a high-quality reference genome sequence and the advent of high-throughput sequencing have greatly empowered re-sequencing studies in maize. In this study, plants...

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Autores principales: Martin Mascher, Nina Gerlach, Manfred Gahrtz, Marcel Bucher, Uwe Scholz, Thomas Dresselhaus
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Publicado: Public Library of Science (PLoS) 2014
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spelling oai:doaj.org-article:ab294c21ffc94bd8835d2d4854491a1d2021-11-18T08:20:25ZSequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.1932-620310.1371/journal.pone.0096782https://doaj.org/article/ab294c21ffc94bd8835d2d4854491a1d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24804793/?tool=EBIhttps://doaj.org/toc/1932-6203Maize (Zea mays) is the most widely grown crop species in the world and a classical model organism for plant research. The completion of a high-quality reference genome sequence and the advent of high-throughput sequencing have greatly empowered re-sequencing studies in maize. In this study, plants of maize inbred line B73 descended from two different sets of seed material grown for several generations either in the field or in the greenhouse were found to show a different growth phenotype and ionome under phosphate starvation conditions and moreover a different responsiveness towards mycorrhizal fungi of the species Glomus intraradices (syn: Rhizophagus irregularis). Whole genome re-sequencing of individuals from both sets and comparison to the B73 reference sequence revealed three cryptic introgressions on chromosomes 1, 5 and 10 in the line grown in the greenhouse summing up to a total of 5,257 single-nucleotide polymorphisms (SNPs). Transcriptome sequencing of three individuals from each set lent further support to the location of the introgression intervals and confirmed them to be fixed in all sequenced individuals. Moreover, we identified >120 genes differentially expressed between the two B73 lines. We thus have found a nearly-isogenic line (NIL) of maize inbred line B73 that is characterized by an altered growth phenotype under phosphate starvation conditions and an improved responsiveness towards symbiosis with mycorrhizal fungi. Through next-generation sequencing of the genomes and transcriptomes we were able to delineate exact introgression intervals. Putative de novo mutations appeared approximately uniformly distributed along the ten maize chromosomes mainly representing G:C -> A:T transitions. The plant material described in this study will be a valuable tool both for functional studies of genes differentially expressed in both B73 lines and for research on growth behavior especially in response to symbiosis between maize and mycorrhizal fungi.Martin MascherNina GerlachManfred GahrtzMarcel BucherUwe ScholzThomas DresselhausPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96782 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Martin Mascher
Nina Gerlach
Manfred Gahrtz
Marcel Bucher
Uwe Scholz
Thomas Dresselhaus
Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.
description Maize (Zea mays) is the most widely grown crop species in the world and a classical model organism for plant research. The completion of a high-quality reference genome sequence and the advent of high-throughput sequencing have greatly empowered re-sequencing studies in maize. In this study, plants of maize inbred line B73 descended from two different sets of seed material grown for several generations either in the field or in the greenhouse were found to show a different growth phenotype and ionome under phosphate starvation conditions and moreover a different responsiveness towards mycorrhizal fungi of the species Glomus intraradices (syn: Rhizophagus irregularis). Whole genome re-sequencing of individuals from both sets and comparison to the B73 reference sequence revealed three cryptic introgressions on chromosomes 1, 5 and 10 in the line grown in the greenhouse summing up to a total of 5,257 single-nucleotide polymorphisms (SNPs). Transcriptome sequencing of three individuals from each set lent further support to the location of the introgression intervals and confirmed them to be fixed in all sequenced individuals. Moreover, we identified >120 genes differentially expressed between the two B73 lines. We thus have found a nearly-isogenic line (NIL) of maize inbred line B73 that is characterized by an altered growth phenotype under phosphate starvation conditions and an improved responsiveness towards symbiosis with mycorrhizal fungi. Through next-generation sequencing of the genomes and transcriptomes we were able to delineate exact introgression intervals. Putative de novo mutations appeared approximately uniformly distributed along the ten maize chromosomes mainly representing G:C -> A:T transitions. The plant material described in this study will be a valuable tool both for functional studies of genes differentially expressed in both B73 lines and for research on growth behavior especially in response to symbiosis between maize and mycorrhizal fungi.
format article
author Martin Mascher
Nina Gerlach
Manfred Gahrtz
Marcel Bucher
Uwe Scholz
Thomas Dresselhaus
author_facet Martin Mascher
Nina Gerlach
Manfred Gahrtz
Marcel Bucher
Uwe Scholz
Thomas Dresselhaus
author_sort Martin Mascher
title Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.
title_short Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.
title_full Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.
title_fullStr Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.
title_full_unstemmed Sequence and ionomic analysis of divergent strains of maize inbred line B73 with an altered growth phenotype.
title_sort sequence and ionomic analysis of divergent strains of maize inbred line b73 with an altered growth phenotype.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/ab294c21ffc94bd8835d2d4854491a1d
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AT ninagerlach sequenceandionomicanalysisofdivergentstrainsofmaizeinbredlineb73withanalteredgrowthphenotype
AT manfredgahrtz sequenceandionomicanalysisofdivergentstrainsofmaizeinbredlineb73withanalteredgrowthphenotype
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