Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis

Abstract Cytomegalovirus retinochoroiditis (CMV-R) is the primary cause of blindness among AIDS patients. Since HLA-G is associated with the modulation of the immune response, we hypothesized that variability at the 3′ untraslated region (3′UTR) of the gene could be implicated on the predisposition...

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Autores principales:	Luciana de Morais Vicente, Erick C. Castelli, Maria de Lourdes Veronese Rodrigues, Neifi Hassan Saloum Deghaide, Celso Teixeira Mendes-Junior, João M. Furtado, Eduardo Antonio Donadi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab2e1992cc9441b18f9b07c8a415dd25
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spelling	oai:doaj.org-article:ab2e1992cc9441b18f9b07c8a415dd252021-12-02T15:09:48ZVariability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis10.1038/s41598-020-75639-92045-2322https://doaj.org/article/ab2e1992cc9441b18f9b07c8a415dd252020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-75639-9https://doaj.org/toc/2045-2322Abstract Cytomegalovirus retinochoroiditis (CMV-R) is the primary cause of blindness among AIDS patients. Since HLA-G is associated with the modulation of the immune response, we hypothesized that variability at the 3′ untraslated region (3′UTR) of the gene could be implicated on the predisposition to CMV-R. We evaluated whether HLA-G 3′UTR influences CMV-R development in Brazilian AIDS patients. Peripheral blood DNA was obtained from two groups of patients: (1) AIDS exhibiting CMV-R (n = 40) and (2) AIDS without CMV-R (n = 147). HLA-G 3′UTR typing was performed using sequencing analysis. Allele, genotype and haplotype frequencies were evaluated using Fisher’s exact test accompanied by the calculation of the odds ratio and its 95% confidence interval (95% CI). The etiologic (EF) and preventive fractions were also estimated. Compared to AIDS patients without CMV-R, AIDS patients with CMV-R showed increased frequencies of the: (1) + 3001T allele, (2) the + 3001C/T genotype and (3) the UTR-17 (InsTTCCGTGACG) haplotype (EFs = 0.02–0.04). The UTR-3 (DelTCCGCGACG) haplotype was associated with protection against CMV-R development. Although the risk for developing CMR-V at the population level was relatively low (EF), the identification of HLA-G 3′UTR variation sites may help to further evaluate the role of post-transcriptional factors that may contribute to the existent immunosuppresion caused by HIV per se.Luciana de Morais VicenteErick C. CastelliMaria de Lourdes Veronese RodriguesNeifi Hassan Saloum DeghaideCelso Teixeira Mendes-JuniorJoão M. FurtadoEduardo Antonio DonadiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luciana de Morais Vicente Erick C. Castelli Maria de Lourdes Veronese Rodrigues Neifi Hassan Saloum Deghaide Celso Teixeira Mendes-Junior João M. Furtado Eduardo Antonio Donadi Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
description	Abstract Cytomegalovirus retinochoroiditis (CMV-R) is the primary cause of blindness among AIDS patients. Since HLA-G is associated with the modulation of the immune response, we hypothesized that variability at the 3′ untraslated region (3′UTR) of the gene could be implicated on the predisposition to CMV-R. We evaluated whether HLA-G 3′UTR influences CMV-R development in Brazilian AIDS patients. Peripheral blood DNA was obtained from two groups of patients: (1) AIDS exhibiting CMV-R (n = 40) and (2) AIDS without CMV-R (n = 147). HLA-G 3′UTR typing was performed using sequencing analysis. Allele, genotype and haplotype frequencies were evaluated using Fisher’s exact test accompanied by the calculation of the odds ratio and its 95% confidence interval (95% CI). The etiologic (EF) and preventive fractions were also estimated. Compared to AIDS patients without CMV-R, AIDS patients with CMV-R showed increased frequencies of the: (1) + 3001T allele, (2) the + 3001C/T genotype and (3) the UTR-17 (InsTTCCGTGACG) haplotype (EFs = 0.02–0.04). The UTR-3 (DelTCCGCGACG) haplotype was associated with protection against CMV-R development. Although the risk for developing CMR-V at the population level was relatively low (EF), the identification of HLA-G 3′UTR variation sites may help to further evaluate the role of post-transcriptional factors that may contribute to the existent immunosuppresion caused by HIV per se.
format	article
author	Luciana de Morais Vicente Erick C. Castelli Maria de Lourdes Veronese Rodrigues Neifi Hassan Saloum Deghaide Celso Teixeira Mendes-Junior João M. Furtado Eduardo Antonio Donadi
author_facet	Luciana de Morais Vicente Erick C. Castelli Maria de Lourdes Veronese Rodrigues Neifi Hassan Saloum Deghaide Celso Teixeira Mendes-Junior João M. Furtado Eduardo Antonio Donadi
author_sort	Luciana de Morais Vicente
title	Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
title_short	Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
title_full	Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
title_fullStr	Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
title_full_unstemmed	Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
title_sort	variability at the 3′ untranslated region of the hla-g gene: a study on patients with aids and cytomegalovirus retinochoroiditis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ab2e1992cc9441b18f9b07c8a415dd25
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Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis

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