Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma

Yifan Zhu,1,&ast; Lisheng Wang,1,&ast; Yanying Nong,2 Yunxiao Liang,3 Zongsheng Huang,3 Pingchuan Zhu,4 Qisong Zhang1 1Medical College of Guangxi University, Guangxi University, Nanning, Guangxi, 530004, People’s Republic of China; 2Department of Gastroenterology, Ruikang Hospital Affiliated...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhu Y, Wang L, Nong Y, Liang Y, Huang Z, Zhu P, Zhang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	colorectal advanced adenoma biomarker serum lipidomics uhplc-hrms colorectal cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/ab3bb8727d8b4e349eb731b8538f7c7c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ab3bb8727d8b4e349eb731b8538f7c7c
record_format	dspace
spelling	oai:doaj.org-article:ab3bb8727d8b4e349eb731b8538f7c7c2021-11-25T18:55:29ZSerum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma1179-1322https://doaj.org/article/ab3bb8727d8b4e349eb731b8538f7c7c2021-11-01T00:00:00Zhttps://www.dovepress.com/serum-untargeted-uhplc-hrms-based-lipidomics-to-discover-the-potential-peer-reviewed-fulltext-article-CMARhttps://doaj.org/toc/1179-1322Yifan Zhu,1,&ast; Lisheng Wang,1,&ast; Yanying Nong,2 Yunxiao Liang,3 Zongsheng Huang,3 Pingchuan Zhu,4 Qisong Zhang1 1Medical College of Guangxi University, Guangxi University, Nanning, Guangxi, 530004, People’s Republic of China; 2Department of Gastroenterology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi, 530011, People’s Republic of China; 3Department of Gastroenterology, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, 530021, People’s Republic of China; 4State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Qisong ZhangMedical College of Guangxi University, Guangxi University, No. 100, East Daxue Road, Nanning, Guangxi, 530004, People’s Republic of ChinaTel +86 0771-3387105Email zhangqisong@gxu.edu.cnBackground: As a key precancerous lesion, colorectal advanced adenoma (CAA) is closely related to the occurrence and development of colorectal cancer (CRC). Effective identification of CAA-related biomarkers can prevent CRC morbidity and mortality. Lipids, as an important endogenous substance, have been proved to be involved in the occurrence and development of CRC. Lipidomics is an advanced technique that studies lipid metabolism and biomarkers of diseases. However, there are no lipidomics studies based on large serum samples to explore diagnostic biomarkers for CAA.Methods: An integrated serum lipid profile from 50 normal (NR) and 46 CAA subjects was performed using ultra-high performance liquid chromatography tandem high-resolution mass spectrometry (UHPLC-HRMS). Lipidomic data were acquired for negative and positive ionization modes, respectively. Differential lipids were selected by univariate and multivariate statistics analyses. A receiver operator characteristic curve (ROC) analysis was conducted to evaluate the diagnostic performance of differential lipids.Results: A total of 53 differential lipids were obtained by combining univariate and multivariate statistical analyses (P < 0.05 and VIP > 1). In addition, 12 differential lipids showed good diagnostic performance (AUC > 0.90) for the discrimination of NR and CAA by receiver operating characteristic curve (ROC) analysis. Of them, the performance of PC 44:5 and PC 35:6e presented the outstanding performance (AUC = 1.00, (95% CI, 1.00– 1.00)). Moreover, triglyceride (TAG) had the highest proportion (37.74%) as the major dysregulated lipids in the CAA.Conclusion: This is the first study that profiled serum lipidomics and explored lipid biomarkers with good diagnostic ability of CAA to contribute to the early prevention of CRC. Twelve differential lipids that effectively discriminate between NR and CAA serve as the potential diagnostic markers of CAA. An obvious perturbation of TAG metabolism could be involved in the CAA formation.Keywords: colorectal advanced adenoma, biomarker, serum lipidomics, UHPLC-HRMS, colorectal cancerZhu YWang LNong YLiang YHuang ZZhu PZhang QDove Medical Pressarticlecolorectal advanced adenomabiomarkerserum lipidomicsuhplc-hrmscolorectal cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Management and Research, Vol Volume 13, Pp 8865-8878 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colorectal advanced adenoma biomarker serum lipidomics uhplc-hrms colorectal cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	colorectal advanced adenoma biomarker serum lipidomics uhplc-hrms colorectal cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Zhu Y Wang L Nong Y Liang Y Huang Z Zhu P Zhang Q Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma
description	Yifan Zhu,1,&ast; Lisheng Wang,1,&ast; Yanying Nong,2 Yunxiao Liang,3 Zongsheng Huang,3 Pingchuan Zhu,4 Qisong Zhang1 1Medical College of Guangxi University, Guangxi University, Nanning, Guangxi, 530004, People’s Republic of China; 2Department of Gastroenterology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi, 530011, People’s Republic of China; 3Department of Gastroenterology, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, 530021, People’s Republic of China; 4State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Qisong ZhangMedical College of Guangxi University, Guangxi University, No. 100, East Daxue Road, Nanning, Guangxi, 530004, People’s Republic of ChinaTel +86 0771-3387105Email zhangqisong@gxu.edu.cnBackground: As a key precancerous lesion, colorectal advanced adenoma (CAA) is closely related to the occurrence and development of colorectal cancer (CRC). Effective identification of CAA-related biomarkers can prevent CRC morbidity and mortality. Lipids, as an important endogenous substance, have been proved to be involved in the occurrence and development of CRC. Lipidomics is an advanced technique that studies lipid metabolism and biomarkers of diseases. However, there are no lipidomics studies based on large serum samples to explore diagnostic biomarkers for CAA.Methods: An integrated serum lipid profile from 50 normal (NR) and 46 CAA subjects was performed using ultra-high performance liquid chromatography tandem high-resolution mass spectrometry (UHPLC-HRMS). Lipidomic data were acquired for negative and positive ionization modes, respectively. Differential lipids were selected by univariate and multivariate statistics analyses. A receiver operator characteristic curve (ROC) analysis was conducted to evaluate the diagnostic performance of differential lipids.Results: A total of 53 differential lipids were obtained by combining univariate and multivariate statistical analyses (P < 0.05 and VIP > 1). In addition, 12 differential lipids showed good diagnostic performance (AUC > 0.90) for the discrimination of NR and CAA by receiver operating characteristic curve (ROC) analysis. Of them, the performance of PC 44:5 and PC 35:6e presented the outstanding performance (AUC = 1.00, (95% CI, 1.00– 1.00)). Moreover, triglyceride (TAG) had the highest proportion (37.74%) as the major dysregulated lipids in the CAA.Conclusion: This is the first study that profiled serum lipidomics and explored lipid biomarkers with good diagnostic ability of CAA to contribute to the early prevention of CRC. Twelve differential lipids that effectively discriminate between NR and CAA serve as the potential diagnostic markers of CAA. An obvious perturbation of TAG metabolism could be involved in the CAA formation.Keywords: colorectal advanced adenoma, biomarker, serum lipidomics, UHPLC-HRMS, colorectal cancer
format	article
author	Zhu Y Wang L Nong Y Liang Y Huang Z Zhu P Zhang Q
author_facet	Zhu Y Wang L Nong Y Liang Y Huang Z Zhu P Zhang Q
author_sort	Zhu Y
title	Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma
title_short	Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma
title_full	Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma
title_fullStr	Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma
title_full_unstemmed	Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma
title_sort	serum untargeted uhplc-hrms-based lipidomics to discover the potential biomarker of colorectal advanced adenoma
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/ab3bb8727d8b4e349eb731b8538f7c7c
work_keys_str_mv	AT zhuy serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma AT wangl serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma AT nongy serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma AT liangy serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma AT huangz serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma AT zhup serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma AT zhangq serumuntargeteduhplchrmsbasedlipidomicstodiscoverthepotentialbiomarkerofcolorectaladvancedadenoma
_version_	1718410545600659456

Serum Untargeted UHPLC-HRMS-Based Lipidomics to Discover the Potential Biomarker of Colorectal Advanced Adenoma

Ejemplares similares