An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.

In plants, the effective mobilization of seed nutrient reserves is crucial during germination and for seedling establishment. The Arabidopsis H+-PPase-loss-of-function fugu5 mutants exhibit a reduced number of cells in the cotyledons. This leads to enhanced post-mitotic cell expansion, also known as...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hiromitsu Tabeta, Shunsuke Watanabe, Keita Fukuda, Shizuka Gunji, Mariko Asaoka, Masami Yokota Hirai, Mitsunori Seo, Hirokazu Tsukaya, Ali Ferjani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ab3bfa8490d0447380fbbf166fcef9b5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ab3bfa8490d0447380fbbf166fcef9b5
record_format	dspace
spelling	oai:doaj.org-article:ab3bfa8490d0447380fbbf166fcef9b52021-12-02T20:03:24ZAn auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.1553-73901553-740410.1371/journal.pgen.1009674https://doaj.org/article/ab3bfa8490d0447380fbbf166fcef9b52021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009674https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404In plants, the effective mobilization of seed nutrient reserves is crucial during germination and for seedling establishment. The Arabidopsis H+-PPase-loss-of-function fugu5 mutants exhibit a reduced number of cells in the cotyledons. This leads to enhanced post-mitotic cell expansion, also known as compensated cell enlargement (CCE). While decreased cell numbers have been ascribed to reduced gluconeogenesis from triacylglycerol, the molecular mechanisms underlying CCE remain ill-known. Given the role of indole 3-butyric acid (IBA) in cotyledon development, and because CCE in fugu5 is specifically and completely cancelled by ech2, which shows defective IBA-to-indoleacetic acid (IAA) conversion, IBA has emerged as a potential regulator of CCE. Here, to further illuminate the regulatory role of IBA in CCE, we used a series of high-order mutants that harbored a specific defect in IBA-to-IAA conversion, IBA efflux, IAA signaling, or vacuolar type H+-ATPase (V-ATPase) activity and analyzed the genetic interaction with fugu5-1. We found that while CCE in fugu5 was promoted by IBA, defects in IBA-to-IAA conversion, IAA response, or the V-ATPase activity alone cancelled CCE. Consistently, endogenous IAA in fugu5 reached a level 2.2-fold higher than the WT in 1-week-old seedlings. Finally, the above findings were validated in icl-2, mls-2, pck1-2 and ibr10 mutants, in which CCE was triggered by low sugar contents. This provides a scenario in which following seed germination, the low-sugar-state triggers IAA synthesis, leading to CCE through the activation of the V-ATPase. These findings illustrate how fine-tuning cell and organ size regulation depend on interplays between metabolism and IAA levels in plants.Hiromitsu TabetaShunsuke WatanabeKeita FukudaShizuka GunjiMariko AsaokaMasami Yokota HiraiMitsunori SeoHirokazu TsukayaAli FerjaniPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009674 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Hiromitsu Tabeta Shunsuke Watanabe Keita Fukuda Shizuka Gunji Mariko Asaoka Masami Yokota Hirai Mitsunori Seo Hirokazu Tsukaya Ali Ferjani An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
description	In plants, the effective mobilization of seed nutrient reserves is crucial during germination and for seedling establishment. The Arabidopsis H+-PPase-loss-of-function fugu5 mutants exhibit a reduced number of cells in the cotyledons. This leads to enhanced post-mitotic cell expansion, also known as compensated cell enlargement (CCE). While decreased cell numbers have been ascribed to reduced gluconeogenesis from triacylglycerol, the molecular mechanisms underlying CCE remain ill-known. Given the role of indole 3-butyric acid (IBA) in cotyledon development, and because CCE in fugu5 is specifically and completely cancelled by ech2, which shows defective IBA-to-indoleacetic acid (IAA) conversion, IBA has emerged as a potential regulator of CCE. Here, to further illuminate the regulatory role of IBA in CCE, we used a series of high-order mutants that harbored a specific defect in IBA-to-IAA conversion, IBA efflux, IAA signaling, or vacuolar type H+-ATPase (V-ATPase) activity and analyzed the genetic interaction with fugu5-1. We found that while CCE in fugu5 was promoted by IBA, defects in IBA-to-IAA conversion, IAA response, or the V-ATPase activity alone cancelled CCE. Consistently, endogenous IAA in fugu5 reached a level 2.2-fold higher than the WT in 1-week-old seedlings. Finally, the above findings were validated in icl-2, mls-2, pck1-2 and ibr10 mutants, in which CCE was triggered by low sugar contents. This provides a scenario in which following seed germination, the low-sugar-state triggers IAA synthesis, leading to CCE through the activation of the V-ATPase. These findings illustrate how fine-tuning cell and organ size regulation depend on interplays between metabolism and IAA levels in plants.
format	article
author	Hiromitsu Tabeta Shunsuke Watanabe Keita Fukuda Shizuka Gunji Mariko Asaoka Masami Yokota Hirai Mitsunori Seo Hirokazu Tsukaya Ali Ferjani
author_facet	Hiromitsu Tabeta Shunsuke Watanabe Keita Fukuda Shizuka Gunji Mariko Asaoka Masami Yokota Hirai Mitsunori Seo Hirokazu Tsukaya Ali Ferjani
author_sort	Hiromitsu Tabeta
title	An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
title_short	An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
title_full	An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
title_fullStr	An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
title_full_unstemmed	An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
title_sort	auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ab3bfa8490d0447380fbbf166fcef9b5
work_keys_str_mv	AT hiromitsutabeta anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT shunsukewatanabe anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT keitafukuda anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT shizukagunji anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT marikoasaoka anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT masamiyokotahirai anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT mitsunoriseo anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT hirokazutsukaya anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT aliferjani anauxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT hiromitsutabeta auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT shunsukewatanabe auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT keitafukuda auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT shizukagunji auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT marikoasaoka auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT masamiyokotahirai auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT mitsunoriseo auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT hirokazutsukaya auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon AT aliferjani auxinsignalingnetworktranslateslowsugarstateinputintocompensatedcellenlargementinthefugu5cotyledon
_version_	1718375678657691648

An auxin signaling network translates low-sugar-state input into compensated cell enlargement in the fugu5 cotyledon.

Ejemplares similares