Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot

Locally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusa...

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Autores principales:	Alessandro Passera, Alessia Follador, Stefano Morandi, Niccolò Miotti, Martina Ghidoli, Giovanni Venturini, Fabio Quaglino, Milena Brasca, Paola Casati, Roberto Pilu, Davide Bulgarelli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Fusarium verticillioides</i> 16S metabarcoding digital PCR RAPD Firmicutes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ab413c4eac9b4a2b820e78ffb6420047
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spelling	oai:doaj.org-article:ab413c4eac9b4a2b820e78ffb64200472021-11-25T18:25:36ZBacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot10.3390/microorganisms91123882076-2607https://doaj.org/article/ab413c4eac9b4a2b820e78ffb64200472021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2388https://doaj.org/toc/2076-2607Locally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusarium ear rot, and whether this trait could be related to particular compositions of the bacterial microbiota in the embryo, using different approaches. Our cultivation-independent approach utilized the metabarcoding of a portion of the 16S rRNA gene to study bacterial populations in these samples. Multivariate statistical analyses indicated that the microbiota of the embryos of the accessions grouped in two different clusters: one comprising three landraces and the hybrid, one including the remaining two landraces, which showed a lower susceptibility to fusarium ear rot in field. The main discriminant between these clusters was the frequency of Firmicutes, higher in the second cluster, and this abundance was confirmed by quantification through digital PCR. The cultivation-dependent approach allowed the isolation of 70 bacterial strains, mostly Firmicutes. In vivo assays allowed the identification of five candidate biocontrol strains against fusarium ear rot. Our data revealed novel insights into the role of the maize embryo microbiota and set the stage for further studies aimed at integrating this knowledge into plant breeding programs.Alessandro PasseraAlessia FolladorStefano MorandiNiccolò MiottiMartina GhidoliGiovanni VenturiniFabio QuaglinoMilena BrascaPaola CasatiRoberto PiluDavide BulgarelliMDPI AGarticle<i>Fusarium verticillioides</i>16S metabarcodingdigital PCRRAPDFirmicutesBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2388, p 2388 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Fusarium verticillioides</i> 16S metabarcoding digital PCR RAPD Firmicutes Biology (General) QH301-705.5
spellingShingle	<i>Fusarium verticillioides</i> 16S metabarcoding digital PCR RAPD Firmicutes Biology (General) QH301-705.5 Alessandro Passera Alessia Follador Stefano Morandi Niccolò Miotti Martina Ghidoli Giovanni Venturini Fabio Quaglino Milena Brasca Paola Casati Roberto Pilu Davide Bulgarelli Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
description	Locally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusarium ear rot, and whether this trait could be related to particular compositions of the bacterial microbiota in the embryo, using different approaches. Our cultivation-independent approach utilized the metabarcoding of a portion of the 16S rRNA gene to study bacterial populations in these samples. Multivariate statistical analyses indicated that the microbiota of the embryos of the accessions grouped in two different clusters: one comprising three landraces and the hybrid, one including the remaining two landraces, which showed a lower susceptibility to fusarium ear rot in field. The main discriminant between these clusters was the frequency of Firmicutes, higher in the second cluster, and this abundance was confirmed by quantification through digital PCR. The cultivation-dependent approach allowed the isolation of 70 bacterial strains, mostly Firmicutes. In vivo assays allowed the identification of five candidate biocontrol strains against fusarium ear rot. Our data revealed novel insights into the role of the maize embryo microbiota and set the stage for further studies aimed at integrating this knowledge into plant breeding programs.
format	article
author	Alessandro Passera Alessia Follador Stefano Morandi Niccolò Miotti Martina Ghidoli Giovanni Venturini Fabio Quaglino Milena Brasca Paola Casati Roberto Pilu Davide Bulgarelli
author_facet	Alessandro Passera Alessia Follador Stefano Morandi Niccolò Miotti Martina Ghidoli Giovanni Venturini Fabio Quaglino Milena Brasca Paola Casati Roberto Pilu Davide Bulgarelli
author_sort	Alessandro Passera
title	Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
title_short	Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
title_full	Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
title_fullStr	Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
title_full_unstemmed	Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
title_sort	bacterial communities in the embryo of maize landraces: relation with susceptibility to fusarium ear rot
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ab413c4eac9b4a2b820e78ffb6420047
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Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot

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