Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol
Abstract We describe here a novel protocol that sequentially combines venous followed by arterial occlusions to determine muscle blood flow and O2 uptake from a single measurement point using near-infrared spectroscopy (NIRS) during handgrip exercise. NIRS data were obtained from the flexor digitoru...
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Nature Portfolio
2021
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oai:doaj.org-article:ab48d8e0aa38427f8f0215d5ae520ea82021-12-02T14:01:21ZMeasurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol10.1038/s41598-020-79741-w2045-2322https://doaj.org/article/ab48d8e0aa38427f8f0215d5ae520ea82021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79741-whttps://doaj.org/toc/2045-2322Abstract We describe here a novel protocol that sequentially combines venous followed by arterial occlusions to determine muscle blood flow and O2 uptake from a single measurement point using near-infrared spectroscopy (NIRS) during handgrip exercise. NIRS data were obtained from the flexor digitorum superficialis (FDS) muscle on the dominant arm of 15 young, healthy adults (3 women; 26 ± 7 years; 78.6 ± 9.1 kg). Participants completed a series of 15-s static handgrip contractions at 20, 40 and 60% of maximal voluntary contraction (MVC) immediately followed by either a: (i) venous occlusion (VO); (ii); arterial occlusion (AO); or venous then arterial occlusion (COMBO). Each condition was repeated 3 times for each exercise-intensity. The concordance correlation coefficient (CCC) and robust linear mixed effects modeling were used to determine measurement agreement between vascular occlusion conditions. FDS muscle blood flow ( $${\dot{\text{Q}}}_{{{\text{FDS}}}}$$ Q ˙ FDS ) and conductance ( $${\text{C}}_{{{\text{FDS}}}}$$ C FDS ) demonstrated strong absolute agreement between VO and COMBO trials from rest up to 60%MVC, as evidenced by high values for CCC (> 0.82) and a linear relationship between conditions that closely approximated the line-of-identity (perfect agreement). Conversely, although FDS muscle O2 uptake ( $${{\dot {\text{V}}}}{{\text{O}}_{2{\text{FDS}}}}$$ V ˙ O 2 FDS ) displayed “substantial” to “near perfect” agreement between methods across exercise intensities (i.e., CCC > 0.80), there was a tendency for COMBO trials to underestimate $${{\dot {\text{V}}}}{{\text{O}}_{2{\text{FDS}}}}$$ V ˙ O 2 FDS by up to 7%. These findings indicate that the COMBO method provides valid estimates of $${\dot {\text{Q}}}_{{\text{FDS}}}$$ Q ˙ FDS and, to a slightly lesser extent, $${{\dot {\text{V}}}}{{\text{O}}_{2{\text{FDS}}}}$$ V ˙ O 2 FDS at rest and during static handgrip exercise up to 60%MVC. Practical implications and suggested improvements of the method are discussed.Joshua J. DennisChad C. WigginsJoshua R. SmithJennifer M. J. IsautierBruce D. JohnsonMichael J. JoynerTroy J. CrossNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Joshua J. Dennis Chad C. Wiggins Joshua R. Smith Jennifer M. J. Isautier Bruce D. Johnson Michael J. Joyner Troy J. Cross Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| description |
Abstract We describe here a novel protocol that sequentially combines venous followed by arterial occlusions to determine muscle blood flow and O2 uptake from a single measurement point using near-infrared spectroscopy (NIRS) during handgrip exercise. NIRS data were obtained from the flexor digitorum superficialis (FDS) muscle on the dominant arm of 15 young, healthy adults (3 women; 26 ± 7 years; 78.6 ± 9.1 kg). Participants completed a series of 15-s static handgrip contractions at 20, 40 and 60% of maximal voluntary contraction (MVC) immediately followed by either a: (i) venous occlusion (VO); (ii); arterial occlusion (AO); or venous then arterial occlusion (COMBO). Each condition was repeated 3 times for each exercise-intensity. The concordance correlation coefficient (CCC) and robust linear mixed effects modeling were used to determine measurement agreement between vascular occlusion conditions. FDS muscle blood flow ( $${\dot{\text{Q}}}_{{{\text{FDS}}}}$$ Q ˙ FDS ) and conductance ( $${\text{C}}_{{{\text{FDS}}}}$$ C FDS ) demonstrated strong absolute agreement between VO and COMBO trials from rest up to 60%MVC, as evidenced by high values for CCC (> 0.82) and a linear relationship between conditions that closely approximated the line-of-identity (perfect agreement). Conversely, although FDS muscle O2 uptake ( $${{\dot {\text{V}}}}{{\text{O}}_{2{\text{FDS}}}}$$ V ˙ O 2 FDS ) displayed “substantial” to “near perfect” agreement between methods across exercise intensities (i.e., CCC > 0.80), there was a tendency for COMBO trials to underestimate $${{\dot {\text{V}}}}{{\text{O}}_{2{\text{FDS}}}}$$ V ˙ O 2 FDS by up to 7%. These findings indicate that the COMBO method provides valid estimates of $${\dot {\text{Q}}}_{{\text{FDS}}}$$ Q ˙ FDS and, to a slightly lesser extent, $${{\dot {\text{V}}}}{{\text{O}}_{2{\text{FDS}}}}$$ V ˙ O 2 FDS at rest and during static handgrip exercise up to 60%MVC. Practical implications and suggested improvements of the method are discussed. |
| format |
article |
| author |
Joshua J. Dennis Chad C. Wiggins Joshua R. Smith Jennifer M. J. Isautier Bruce D. Johnson Michael J. Joyner Troy J. Cross |
| author_facet |
Joshua J. Dennis Chad C. Wiggins Joshua R. Smith Jennifer M. J. Isautier Bruce D. Johnson Michael J. Joyner Troy J. Cross |
| author_sort |
Joshua J. Dennis |
| title |
Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| title_short |
Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| title_full |
Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| title_fullStr |
Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| title_full_unstemmed |
Measurement of muscle blood flow and O2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| title_sort |
measurement of muscle blood flow and o2 uptake via near-infrared spectroscopy using a novel occlusion protocol |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/ab48d8e0aa38427f8f0215d5ae520ea8 |
| work_keys_str_mv |
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