Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments

Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation...

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Autores principales:	Yu Pan, Zhao Liu, Yijie Wang, Linshen Zhang, Niying Chua, Lei Dai, Jun Chen, Chun Loong Ho
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lactoferrin anti-infammatory protease-digested non-malignant colonic fibroblasts cells tNF-alpha Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ab4ac9c866cc459ead9bf00b13aabf85
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spelling	oai:doaj.org-article:ab4ac9c866cc459ead9bf00b13aabf852021-12-01T19:10:50ZEvaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments2296-418510.3389/fbioe.2021.779018https://doaj.org/article/ab4ac9c866cc459ead9bf00b13aabf852021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.779018/fullhttps://doaj.org/toc/2296-4185Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation. Protease-digested lactoferrin fragments often exhibit improved therapeutic properties compared to full-length lactoferrin (flHLF). However, there are no studies on the use of protease-digested lactoferrin fragments to treat inflammation. Herein, we assess the anti-inflammatory properties of engineered recombinant lactoferrin fragments (rtHLF4, rteHLF1, and rpHLF2) on non-malignant colonic fibroblast cells and colorectal cancer cells. We found that rtHLF4 is 10 times more effective to prevent inflammation compared to flHLF. These results were investigated by looking into the reactive oxygen species (ROS) production, angiogenesis activity, and cellular proliferation of the treated cells. We have demonstrated in this study the anti-inflammatory properties of the flHLF and the various lactoferrin fragments. These results complement the anti-cancer properties of these proteins that were demonstrated in an earlier study.Yu PanZhao LiuYijie WangLinshen ZhangNiying ChuaLei DaiJun ChenChun Loong HoFrontiers Media S.A.articlelactoferrinanti-infammatoryprotease-digestednon-malignant colonic fibroblasts cellstNF-alphaBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lactoferrin anti-infammatory protease-digested non-malignant colonic fibroblasts cells tNF-alpha Biotechnology TP248.13-248.65
spellingShingle	lactoferrin anti-infammatory protease-digested non-malignant colonic fibroblasts cells tNF-alpha Biotechnology TP248.13-248.65 Yu Pan Zhao Liu Yijie Wang Linshen Zhang Niying Chua Lei Dai Jun Chen Chun Loong Ho Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments
description	Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation. Protease-digested lactoferrin fragments often exhibit improved therapeutic properties compared to full-length lactoferrin (flHLF). However, there are no studies on the use of protease-digested lactoferrin fragments to treat inflammation. Herein, we assess the anti-inflammatory properties of engineered recombinant lactoferrin fragments (rtHLF4, rteHLF1, and rpHLF2) on non-malignant colonic fibroblast cells and colorectal cancer cells. We found that rtHLF4 is 10 times more effective to prevent inflammation compared to flHLF. These results were investigated by looking into the reactive oxygen species (ROS) production, angiogenesis activity, and cellular proliferation of the treated cells. We have demonstrated in this study the anti-inflammatory properties of the flHLF and the various lactoferrin fragments. These results complement the anti-cancer properties of these proteins that were demonstrated in an earlier study.
format	article
author	Yu Pan Zhao Liu Yijie Wang Linshen Zhang Niying Chua Lei Dai Jun Chen Chun Loong Ho
author_facet	Yu Pan Zhao Liu Yijie Wang Linshen Zhang Niying Chua Lei Dai Jun Chen Chun Loong Ho
author_sort	Yu Pan
title	Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments
title_short	Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments
title_full	Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments
title_fullStr	Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments
title_full_unstemmed	Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments
title_sort	evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ab4ac9c866cc459ead9bf00b13aabf85
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Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments

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