Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
<i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Sinc...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/ab4da8c01a024268a1b9daaf484ddeb6 |
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| Sumario: | <i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Since only few <i>Yersinia</i> phages have been described yet, some of which demonstrated a high specificity for certain serotypes, we isolated two phages from game animals and characterized them in terms of their morphology, host specificity, lytic activity on two bio-/serotypes and genome composition. The T7-related podovirus vB_YenP_Rambo and the myovirus vB_YenM_P281, which is very similar to a previously described phage PY100, showed a broad host range. Together, they lysed all the 62 tested pathogenic <i>Y. enterocolitica</i> strains belonging to the most important bio-/serotypes in Europe. A cocktail containing these two phages strongly reduced cultures of a bio-/serotype B4/O:3 and a B2/O:9 strain, even at very low MOIs (multiplicity of infection) and different temperatures, though, lysis of bio-/serotype B2/O:9 by vB_YenM_P281 and also by the related phage PY100 only occurred at 37 °C. Both phages were additionally able to lyse various <i>Y. pseudotuberculosis</i> strains at 28 °C and 37 °C, but only when the growth medium was supplemented with calcium and magnesium cations. |
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