Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals

<i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Sinc...

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Autores principales: Jens A. Hammerl, Andrea Barac, Philipp Erben, Julius Fuhrmann, Ashish Gadicherla, Franziska Kumsteller, Anne Lauckner, Felix Müller, Stefan Hertwig
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:ab4da8c01a024268a1b9daaf484ddeb62021-11-11T16:51:19ZProperties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals10.3390/ijms2221113811422-00671661-6596https://doaj.org/article/ab4da8c01a024268a1b9daaf484ddeb62021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11381https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Since only few <i>Yersinia</i> phages have been described yet, some of which demonstrated a high specificity for certain serotypes, we isolated two phages from game animals and characterized them in terms of their morphology, host specificity, lytic activity on two bio-/serotypes and genome composition. The T7-related podovirus vB_YenP_Rambo and the myovirus vB_YenM_P281, which is very similar to a previously described phage PY100, showed a broad host range. Together, they lysed all the 62 tested pathogenic <i>Y. enterocolitica</i> strains belonging to the most important bio-/serotypes in Europe. A cocktail containing these two phages strongly reduced cultures of a bio-/serotype B4/O:3 and a B2/O:9 strain, even at very low MOIs (multiplicity of infection) and different temperatures, though, lysis of bio-/serotype B2/O:9 by vB_YenM_P281 and also by the related phage PY100 only occurred at 37 °C. Both phages were additionally able to lyse various <i>Y. pseudotuberculosis</i> strains at 28 °C and 37 °C, but only when the growth medium was supplemented with calcium and magnesium cations.Jens A. HammerlAndrea BaracPhilipp ErbenJulius FuhrmannAshish GadicherlaFranziska KumstellerAnne LaucknerFelix MüllerStefan HertwigMDPI AGarticle<i>Yersinia enterocolitica</i>phagegenomeapplicationtherapyBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11381, p 11381 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Yersinia enterocolitica</i>
phage
genome
application
therapy
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle <i>Yersinia enterocolitica</i>
phage
genome
application
therapy
Biology (General)
QH301-705.5
Chemistry
QD1-999
Jens A. Hammerl
Andrea Barac
Philipp Erben
Julius Fuhrmann
Ashish Gadicherla
Franziska Kumsteller
Anne Lauckner
Felix Müller
Stefan Hertwig
Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
description <i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Since only few <i>Yersinia</i> phages have been described yet, some of which demonstrated a high specificity for certain serotypes, we isolated two phages from game animals and characterized them in terms of their morphology, host specificity, lytic activity on two bio-/serotypes and genome composition. The T7-related podovirus vB_YenP_Rambo and the myovirus vB_YenM_P281, which is very similar to a previously described phage PY100, showed a broad host range. Together, they lysed all the 62 tested pathogenic <i>Y. enterocolitica</i> strains belonging to the most important bio-/serotypes in Europe. A cocktail containing these two phages strongly reduced cultures of a bio-/serotype B4/O:3 and a B2/O:9 strain, even at very low MOIs (multiplicity of infection) and different temperatures, though, lysis of bio-/serotype B2/O:9 by vB_YenM_P281 and also by the related phage PY100 only occurred at 37 °C. Both phages were additionally able to lyse various <i>Y. pseudotuberculosis</i> strains at 28 °C and 37 °C, but only when the growth medium was supplemented with calcium and magnesium cations.
format article
author Jens A. Hammerl
Andrea Barac
Philipp Erben
Julius Fuhrmann
Ashish Gadicherla
Franziska Kumsteller
Anne Lauckner
Felix Müller
Stefan Hertwig
author_facet Jens A. Hammerl
Andrea Barac
Philipp Erben
Julius Fuhrmann
Ashish Gadicherla
Franziska Kumsteller
Anne Lauckner
Felix Müller
Stefan Hertwig
author_sort Jens A. Hammerl
title Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
title_short Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
title_full Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
title_fullStr Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
title_full_unstemmed Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
title_sort properties of two broad host range phages of <i>yersinia enterocolitica</i> isolated from wild animals
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/ab4da8c01a024268a1b9daaf484ddeb6
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