Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals
<i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Sinc...
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oai:doaj.org-article:ab4da8c01a024268a1b9daaf484ddeb62021-11-11T16:51:19ZProperties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals10.3390/ijms2221113811422-00671661-6596https://doaj.org/article/ab4da8c01a024268a1b9daaf484ddeb62021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11381https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Since only few <i>Yersinia</i> phages have been described yet, some of which demonstrated a high specificity for certain serotypes, we isolated two phages from game animals and characterized them in terms of their morphology, host specificity, lytic activity on two bio-/serotypes and genome composition. The T7-related podovirus vB_YenP_Rambo and the myovirus vB_YenM_P281, which is very similar to a previously described phage PY100, showed a broad host range. Together, they lysed all the 62 tested pathogenic <i>Y. enterocolitica</i> strains belonging to the most important bio-/serotypes in Europe. A cocktail containing these two phages strongly reduced cultures of a bio-/serotype B4/O:3 and a B2/O:9 strain, even at very low MOIs (multiplicity of infection) and different temperatures, though, lysis of bio-/serotype B2/O:9 by vB_YenM_P281 and also by the related phage PY100 only occurred at 37 °C. Both phages were additionally able to lyse various <i>Y. pseudotuberculosis</i> strains at 28 °C and 37 °C, but only when the growth medium was supplemented with calcium and magnesium cations.Jens A. HammerlAndrea BaracPhilipp ErbenJulius FuhrmannAshish GadicherlaFranziska KumstellerAnne LaucknerFelix MüllerStefan HertwigMDPI AGarticle<i>Yersinia enterocolitica</i>phagegenomeapplicationtherapyBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11381, p 11381 (2021) |
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<i>Yersinia enterocolitica</i> phage genome application therapy Biology (General) QH301-705.5 Chemistry QD1-999 |
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<i>Yersinia enterocolitica</i> phage genome application therapy Biology (General) QH301-705.5 Chemistry QD1-999 Jens A. Hammerl Andrea Barac Philipp Erben Julius Fuhrmann Ashish Gadicherla Franziska Kumsteller Anne Lauckner Felix Müller Stefan Hertwig Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals |
description |
<i>Yersinia</i> (<i>Y</i>.) <i>enterocolitica</i> and <i>Y. pseudotuberculosis</i> are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Since only few <i>Yersinia</i> phages have been described yet, some of which demonstrated a high specificity for certain serotypes, we isolated two phages from game animals and characterized them in terms of their morphology, host specificity, lytic activity on two bio-/serotypes and genome composition. The T7-related podovirus vB_YenP_Rambo and the myovirus vB_YenM_P281, which is very similar to a previously described phage PY100, showed a broad host range. Together, they lysed all the 62 tested pathogenic <i>Y. enterocolitica</i> strains belonging to the most important bio-/serotypes in Europe. A cocktail containing these two phages strongly reduced cultures of a bio-/serotype B4/O:3 and a B2/O:9 strain, even at very low MOIs (multiplicity of infection) and different temperatures, though, lysis of bio-/serotype B2/O:9 by vB_YenM_P281 and also by the related phage PY100 only occurred at 37 °C. Both phages were additionally able to lyse various <i>Y. pseudotuberculosis</i> strains at 28 °C and 37 °C, but only when the growth medium was supplemented with calcium and magnesium cations. |
format |
article |
author |
Jens A. Hammerl Andrea Barac Philipp Erben Julius Fuhrmann Ashish Gadicherla Franziska Kumsteller Anne Lauckner Felix Müller Stefan Hertwig |
author_facet |
Jens A. Hammerl Andrea Barac Philipp Erben Julius Fuhrmann Ashish Gadicherla Franziska Kumsteller Anne Lauckner Felix Müller Stefan Hertwig |
author_sort |
Jens A. Hammerl |
title |
Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals |
title_short |
Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals |
title_full |
Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals |
title_fullStr |
Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals |
title_full_unstemmed |
Properties of Two Broad Host Range Phages of <i>Yersinia enterocolitica</i> Isolated from Wild Animals |
title_sort |
properties of two broad host range phages of <i>yersinia enterocolitica</i> isolated from wild animals |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/ab4da8c01a024268a1b9daaf484ddeb6 |
work_keys_str_mv |
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