Identification and quantification of honeybee venom constituents by multiplatform metabolomics

Abstract Honeybee (Apis mellifera) venom (HBV) has been a subject of extensive proteomics research; however, scarce information on its metabolite composition can be found in the literature. The aim of the study was to identify and quantify the metabolites present in HBV. To gain the highest metaboli...

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Autores principales:	Agnieszka Klupczynska, Szymon Plewa, Paweł Dereziński, Timothy J. Garrett, Vanessa Y. Rubio, Zenon J. Kokot, Jan Matysiak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab5a2c0bdf7d40cc9533c5c7d645f3fd
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spelling	oai:doaj.org-article:ab5a2c0bdf7d40cc9533c5c7d645f3fd2021-12-02T15:11:52ZIdentification and quantification of honeybee venom constituents by multiplatform metabolomics10.1038/s41598-020-78740-12045-2322https://doaj.org/article/ab5a2c0bdf7d40cc9533c5c7d645f3fd2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78740-1https://doaj.org/toc/2045-2322Abstract Honeybee (Apis mellifera) venom (HBV) has been a subject of extensive proteomics research; however, scarce information on its metabolite composition can be found in the literature. The aim of the study was to identify and quantify the metabolites present in HBV. To gain the highest metabolite coverage, three different mass spectrometry (MS)-based methodologies were applied. In the first step, untargeted metabolomics was used, which employed high-resolution, accurate-mass Orbitrap MS. It allowed obtaining a broad overview of HBV metabolic components. Then, two targeted metabolomics approaches, which employed triple quadrupole MS, were applied to quantify metabolites in HBV samples. The untargeted metabolomics not only confirmed the presence of amines, amino acids, carbohydrates, and organic acids in HBV, but also provided information on venom components from other metabolite classes (e.g., nucleosides, alcohols, purine and pyrimidine derivatives). The combination of three MS-based metabolomics platforms facilitated the identification of 214 metabolites in HBV samples, among which 138 were quantified. The obtaining of the wide free amino acid profiles of HBV is one of the project’s achievements. Our study contributed significantly to broadening the knowledge about HBV composition and should be continued to obtain the most comprehensive metabolite profile of HBV.Agnieszka KlupczynskaSzymon PlewaPaweł DerezińskiTimothy J. GarrettVanessa Y. RubioZenon J. KokotJan MatysiakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka Klupczynska Szymon Plewa Paweł Dereziński Timothy J. Garrett Vanessa Y. Rubio Zenon J. Kokot Jan Matysiak Identification and quantification of honeybee venom constituents by multiplatform metabolomics
description	Abstract Honeybee (Apis mellifera) venom (HBV) has been a subject of extensive proteomics research; however, scarce information on its metabolite composition can be found in the literature. The aim of the study was to identify and quantify the metabolites present in HBV. To gain the highest metabolite coverage, three different mass spectrometry (MS)-based methodologies were applied. In the first step, untargeted metabolomics was used, which employed high-resolution, accurate-mass Orbitrap MS. It allowed obtaining a broad overview of HBV metabolic components. Then, two targeted metabolomics approaches, which employed triple quadrupole MS, were applied to quantify metabolites in HBV samples. The untargeted metabolomics not only confirmed the presence of amines, amino acids, carbohydrates, and organic acids in HBV, but also provided information on venom components from other metabolite classes (e.g., nucleosides, alcohols, purine and pyrimidine derivatives). The combination of three MS-based metabolomics platforms facilitated the identification of 214 metabolites in HBV samples, among which 138 were quantified. The obtaining of the wide free amino acid profiles of HBV is one of the project’s achievements. Our study contributed significantly to broadening the knowledge about HBV composition and should be continued to obtain the most comprehensive metabolite profile of HBV.
format	article
author	Agnieszka Klupczynska Szymon Plewa Paweł Dereziński Timothy J. Garrett Vanessa Y. Rubio Zenon J. Kokot Jan Matysiak
author_facet	Agnieszka Klupczynska Szymon Plewa Paweł Dereziński Timothy J. Garrett Vanessa Y. Rubio Zenon J. Kokot Jan Matysiak
author_sort	Agnieszka Klupczynska
title	Identification and quantification of honeybee venom constituents by multiplatform metabolomics
title_short	Identification and quantification of honeybee venom constituents by multiplatform metabolomics
title_full	Identification and quantification of honeybee venom constituents by multiplatform metabolomics
title_fullStr	Identification and quantification of honeybee venom constituents by multiplatform metabolomics
title_full_unstemmed	Identification and quantification of honeybee venom constituents by multiplatform metabolomics
title_sort	identification and quantification of honeybee venom constituents by multiplatform metabolomics
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ab5a2c0bdf7d40cc9533c5c7d645f3fd
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Identification and quantification of honeybee venom constituents by multiplatform metabolomics

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