Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study

Abstract It is increasingly recognized that early detection of bone erosion plays an important role in the overall evaluation of rheumatoid arthritis and in the choice of the correct treatment approach. Since an appropriate use of imaging biomarkers in preclinical settings offers the prospect of sma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sandro Donato, Serena Pacilè, Federico Colombo, Chiara Garrovo, Simeone Dal Monego, Paolo Macor, Giuliana Tromba, Stefania Biffi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab602848eed0499594ed72f0d31587be
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ab602848eed0499594ed72f0d31587be
record_format	dspace
spelling	oai:doaj.org-article:ab602848eed0499594ed72f0d31587be2021-12-02T12:32:41ZMeniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study10.1038/s41598-017-08025-72045-2322https://doaj.org/article/ab602848eed0499594ed72f0d31587be2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08025-7https://doaj.org/toc/2045-2322Abstract It is increasingly recognized that early detection of bone erosion plays an important role in the overall evaluation of rheumatoid arthritis and in the choice of the correct treatment approach. Since an appropriate use of imaging biomarkers in preclinical settings offers the prospect of smaller and optimized sample size, in the present study we define an anatomical imaging biomarker that could be objectively measured from micro-CT imaging data as an indicator of bone erosion in arthritis process. The well-characterized antigen-induced arthritis (AIA) model in rats was used. The animals were divided into 2 groups: arthritic disease control and arthritic having been administrated with the tumor necrosis factor alpha-blocking agent (Humira). Rats were sacrificed in the acute phase of AIA; peripheral blood and synovial tissue were collected for assessment of arthritis. Ex vivo micro-CT tomography of knee joints was performed at the Elettra synchrotron light source (Trieste, Italy). Overall, results from this study suggest that use of high-resolution micro-CT analysis coupled with meniscal ossicles bone parameters quantification provide a powerful combination to enhance data interpretation and assessment of disease-modifying drugs in an animal model of arthritis.Sandro DonatoSerena PacilèFederico ColomboChiara GarrovoSimeone Dal MonegoPaolo MacorGiuliana TrombaStefania BiffiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandro Donato Serena Pacilè Federico Colombo Chiara Garrovo Simeone Dal Monego Paolo Macor Giuliana Tromba Stefania Biffi Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study
description	Abstract It is increasingly recognized that early detection of bone erosion plays an important role in the overall evaluation of rheumatoid arthritis and in the choice of the correct treatment approach. Since an appropriate use of imaging biomarkers in preclinical settings offers the prospect of smaller and optimized sample size, in the present study we define an anatomical imaging biomarker that could be objectively measured from micro-CT imaging data as an indicator of bone erosion in arthritis process. The well-characterized antigen-induced arthritis (AIA) model in rats was used. The animals were divided into 2 groups: arthritic disease control and arthritic having been administrated with the tumor necrosis factor alpha-blocking agent (Humira). Rats were sacrificed in the acute phase of AIA; peripheral blood and synovial tissue were collected for assessment of arthritis. Ex vivo micro-CT tomography of knee joints was performed at the Elettra synchrotron light source (Trieste, Italy). Overall, results from this study suggest that use of high-resolution micro-CT analysis coupled with meniscal ossicles bone parameters quantification provide a powerful combination to enhance data interpretation and assessment of disease-modifying drugs in an animal model of arthritis.
format	article
author	Sandro Donato Serena Pacilè Federico Colombo Chiara Garrovo Simeone Dal Monego Paolo Macor Giuliana Tromba Stefania Biffi
author_facet	Sandro Donato Serena Pacilè Federico Colombo Chiara Garrovo Simeone Dal Monego Paolo Macor Giuliana Tromba Stefania Biffi
author_sort	Sandro Donato
title	Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study
title_short	Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study
title_full	Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study
title_fullStr	Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study
title_full_unstemmed	Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study
title_sort	meniscal ossicles as micro-ct imaging biomarker in a rodent model of antigen-induced arthritis: a synchrotron-based x-ray pilot study
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ab602848eed0499594ed72f0d31587be
work_keys_str_mv	AT sandrodonato meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT serenapacile meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT federicocolombo meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT chiaragarrovo meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT simeonedalmonego meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT paolomacor meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT giulianatromba meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy AT stefaniabiffi meniscalossiclesasmicroctimagingbiomarkerinarodentmodelofantigeninducedarthritisasynchrotronbasedxraypilotstudy
_version_	1718393998872150016

Meniscal Ossicles as micro-CT Imaging Biomarker in a Rodent Model of Antigen-Induced Arthritis: a Synchrotron-Based X-ray Pilot Study

Ejemplares similares