Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction

Testicular development during juvenile is crucial for subsequent male reproductive function. However, it remains poorly understood about the contribution of the testis microenvironment to human germ cell maturation. Therefore, we systematically analyzed scRNA-seq transcriptome and found the dramatic...

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Autores principales:	Ning Zhang, Yinuo Wang, Ziyu Chen, Jianhong Ren, Abdur Rehman, Dawood Warraich Ahmad, Deyu Long, Junyao Hou, Yaqi Zhou, Luyu Yang, Yu Ni, Yayu Li, Changjian Du, Yingcui Yu, Mingzhi Liao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Bisphenol A(BPA) Microenvironment Secretory proteins Single-cell transcriptome Cell-cell communication network Reproductive toxicology Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ab64d00314a646f8a532754836a0e5d9
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spelling	oai:doaj.org-article:ab64d00314a646f8a532754836a0e5d92021-11-22T04:17:55ZSingle-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction0753-332210.1016/j.biopha.2021.112449https://doaj.org/article/ab64d00314a646f8a532754836a0e5d92022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S075333222101235Xhttps://doaj.org/toc/0753-3322Testicular development during juvenile is crucial for subsequent male reproductive function. However, it remains poorly understood about the contribution of the testis microenvironment to human germ cell maturation. Therefore, we systematically analyzed scRNA-seq transcriptome and found the dramatic changes in cell-type composition in human testis during puberty. Then we constructed cell-cell communication networks between germ cells and somatic cells in the juvenile testis, which may be achieved via immune-related pathways. Our results showed that maturation-promoting factors are the switches of the Sertoli cells that drive sperm maturation. Furthermore, we found that Bisphenol A(BPA) enhanced the maturation and growth of germ cells through the Sertoli cell's secretory protein. Finally, our results indicate Bisphenol A would lead to the dysregulation of secreted protein expression in Sertoli cells during spermatogenesis, which in turn has direct cytotoxicity to Sertoli cells. Bisphenol A is one of the underlying causes of non-obstructive azoospermia (NOA). In summary, our results reveal the reproductive toxicity and molecular mechanism of Bisphenol A in Sertoli cells and male reproduction. Provide a reference for the toxicity of Bisphenol A to human reproduction.Ning ZhangYinuo WangZiyu ChenJianhong RenAbdur RehmanDawood Warraich AhmadDeyu LongJunyao HouYaqi ZhouLuyu YangYu NiYayu LiChangjian DuYingcui YuMingzhi LiaoElsevierarticleBisphenol A(BPA)MicroenvironmentSecretory proteinsSingle-cell transcriptomeCell-cell communication networkReproductive toxicologyTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112449- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bisphenol A(BPA) Microenvironment Secretory proteins Single-cell transcriptome Cell-cell communication network Reproductive toxicology Therapeutics. Pharmacology RM1-950
spellingShingle	Bisphenol A(BPA) Microenvironment Secretory proteins Single-cell transcriptome Cell-cell communication network Reproductive toxicology Therapeutics. Pharmacology RM1-950 Ning Zhang Yinuo Wang Ziyu Chen Jianhong Ren Abdur Rehman Dawood Warraich Ahmad Deyu Long Junyao Hou Yaqi Zhou Luyu Yang Yu Ni Yayu Li Changjian Du Yingcui Yu Mingzhi Liao Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction
description	Testicular development during juvenile is crucial for subsequent male reproductive function. However, it remains poorly understood about the contribution of the testis microenvironment to human germ cell maturation. Therefore, we systematically analyzed scRNA-seq transcriptome and found the dramatic changes in cell-type composition in human testis during puberty. Then we constructed cell-cell communication networks between germ cells and somatic cells in the juvenile testis, which may be achieved via immune-related pathways. Our results showed that maturation-promoting factors are the switches of the Sertoli cells that drive sperm maturation. Furthermore, we found that Bisphenol A(BPA) enhanced the maturation and growth of germ cells through the Sertoli cell's secretory protein. Finally, our results indicate Bisphenol A would lead to the dysregulation of secreted protein expression in Sertoli cells during spermatogenesis, which in turn has direct cytotoxicity to Sertoli cells. Bisphenol A is one of the underlying causes of non-obstructive azoospermia (NOA). In summary, our results reveal the reproductive toxicity and molecular mechanism of Bisphenol A in Sertoli cells and male reproduction. Provide a reference for the toxicity of Bisphenol A to human reproduction.
format	article
author	Ning Zhang Yinuo Wang Ziyu Chen Jianhong Ren Abdur Rehman Dawood Warraich Ahmad Deyu Long Junyao Hou Yaqi Zhou Luyu Yang Yu Ni Yayu Li Changjian Du Yingcui Yu Mingzhi Liao
author_facet	Ning Zhang Yinuo Wang Ziyu Chen Jianhong Ren Abdur Rehman Dawood Warraich Ahmad Deyu Long Junyao Hou Yaqi Zhou Luyu Yang Yu Ni Yayu Li Changjian Du Yingcui Yu Mingzhi Liao
author_sort	Ning Zhang
title	Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction
title_short	Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction
title_full	Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction
title_fullStr	Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction
title_full_unstemmed	Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction
title_sort	single-cell transcriptome analysis of bisphenol a exposure reveals the key roles of the testicular microenvironment in male reproduction
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/ab64d00314a646f8a532754836a0e5d9
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Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction

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