Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses

Jakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Departmen...

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Autores principales:	Sirc J, Kubinova S, Hobzova R, Stranska D, Kozlik P, Bosakova Z, Marekova D, Holan V, Sykova E, Michalek J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ab68077eaf6746e780f28db300bcd18e
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spelling	oai:doaj.org-article:ab68077eaf6746e780f28db300bcd18e2021-12-02T00:37:47ZControlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses1176-91141178-2013https://doaj.org/article/ab68077eaf6746e780f28db300bcd18e2012-10-01T00:00:00Zhttp://www.dovepress.com/controlled-gentamicin-release-from-multi-layered-electrospun-nanofibro-a11205https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Department of Neuroscience, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic; 3Elmarco Ltd, Liberec, Czech Republic; 4Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic; 5Department of Transplant Immunology, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague, Czech RepublicAbstract: Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider&trade; needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.Keywords: nanofibers, electrospinning, multilayered structure, morphology, gentamicin, drug releaseSirc JKubinova SHobzova RStranska DKozlik PBosakova ZMarekova DHolan VSykova EMichalek JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5315-5325 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sirc J Kubinova S Hobzova R Stranska D Kozlik P Bosakova Z Marekova D Holan V Sykova E Michalek J Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
description	Jakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Department of Neuroscience, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic; 3Elmarco Ltd, Liberec, Czech Republic; 4Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic; 5Department of Transplant Immunology, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague, Czech RepublicAbstract: Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider&trade; needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.Keywords: nanofibers, electrospinning, multilayered structure, morphology, gentamicin, drug release
format	article
author	Sirc J Kubinova S Hobzova R Stranska D Kozlik P Bosakova Z Marekova D Holan V Sykova E Michalek J
author_facet	Sirc J Kubinova S Hobzova R Stranska D Kozlik P Bosakova Z Marekova D Holan V Sykova E Michalek J
author_sort	Sirc J
title	Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
title_short	Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
title_full	Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
title_fullStr	Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
title_full_unstemmed	Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
title_sort	controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ab68077eaf6746e780f28db300bcd18e
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Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses

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