Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.

Some bacterial group II introns are widely used for genetic engineering in bacteria, because they can be reprogrammed to insert into the desired DNA target sites. There is considerable interest in developing this group II intron gene targeting technology for use in eukaryotes, but nuclear genomes pr...

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Autores principales:	Rafael Nisa-Martínez, Philippe Laporte, José Ignacio Jiménez-Zurdo, Florian Frugier, Martin Crespi, Nicolás Toro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab71968e57af4dc7bf054431a3df2b70
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spelling	oai:doaj.org-article:ab71968e57af4dc7bf054431a3df2b702021-11-18T08:39:26ZLocalization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.1932-620310.1371/journal.pone.0084056https://doaj.org/article/ab71968e57af4dc7bf054431a3df2b702013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391881/?tool=EBIhttps://doaj.org/toc/1932-6203Some bacterial group II introns are widely used for genetic engineering in bacteria, because they can be reprogrammed to insert into the desired DNA target sites. There is considerable interest in developing this group II intron gene targeting technology for use in eukaryotes, but nuclear genomes present several obstacles to the use of this approach. The nuclear genomes of eukaryotes do not contain group II introns, but these introns are thought to have been the progenitors of nuclear spliceosomal introns. We investigated the expression and subcellular localization of the bacterial RmInt1 group II intron-encoded protein (IEP) in Arabidopsis thaliana protoplasts. Following the expression of translational fusions of the wild-type protein and several mutant variants with EGFP, the full-length IEP was found exclusively in the nucleolus, whereas the maturase domain alone targeted EGFP to nuclear speckles. The distribution of the bacterial RmInt1 IEP in plant cell protoplasts suggests that the compartmentalization of eukaryotic cells into nucleus and cytoplasm does not prevent group II introns from invading the host genome. Furthermore, the trafficking of the IEP between the nucleolus and the speckles upon maturase inactivation is consistent with the hypothesis that the spliceosomal machinery evolved from group II introns.Rafael Nisa-MartínezPhilippe LaporteJosé Ignacio Jiménez-ZurdoFlorian FrugierMartin CrespiNicolás ToroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84056 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rafael Nisa-Martínez Philippe Laporte José Ignacio Jiménez-Zurdo Florian Frugier Martin Crespi Nicolás Toro Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
description	Some bacterial group II introns are widely used for genetic engineering in bacteria, because they can be reprogrammed to insert into the desired DNA target sites. There is considerable interest in developing this group II intron gene targeting technology for use in eukaryotes, but nuclear genomes present several obstacles to the use of this approach. The nuclear genomes of eukaryotes do not contain group II introns, but these introns are thought to have been the progenitors of nuclear spliceosomal introns. We investigated the expression and subcellular localization of the bacterial RmInt1 group II intron-encoded protein (IEP) in Arabidopsis thaliana protoplasts. Following the expression of translational fusions of the wild-type protein and several mutant variants with EGFP, the full-length IEP was found exclusively in the nucleolus, whereas the maturase domain alone targeted EGFP to nuclear speckles. The distribution of the bacterial RmInt1 IEP in plant cell protoplasts suggests that the compartmentalization of eukaryotic cells into nucleus and cytoplasm does not prevent group II introns from invading the host genome. Furthermore, the trafficking of the IEP between the nucleolus and the speckles upon maturase inactivation is consistent with the hypothesis that the spliceosomal machinery evolved from group II introns.
format	article
author	Rafael Nisa-Martínez Philippe Laporte José Ignacio Jiménez-Zurdo Florian Frugier Martin Crespi Nicolás Toro
author_facet	Rafael Nisa-Martínez Philippe Laporte José Ignacio Jiménez-Zurdo Florian Frugier Martin Crespi Nicolás Toro
author_sort	Rafael Nisa-Martínez
title	Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
title_short	Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
title_full	Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
title_fullStr	Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
title_full_unstemmed	Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
title_sort	localization of a bacterial group ii intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ab71968e57af4dc7bf054431a3df2b70
work_keys_str_mv	AT rafaelnisamartinez localizationofabacterialgroupiiintronencodedproteinineukaryoticnuclearsplicingrelatedcellcompartments AT philippelaporte localizationofabacterialgroupiiintronencodedproteinineukaryoticnuclearsplicingrelatedcellcompartments AT joseignaciojimenezzurdo localizationofabacterialgroupiiintronencodedproteinineukaryoticnuclearsplicingrelatedcellcompartments AT florianfrugier localizationofabacterialgroupiiintronencodedproteinineukaryoticnuclearsplicingrelatedcellcompartments AT martincrespi localizationofabacterialgroupiiintronencodedproteinineukaryoticnuclearsplicingrelatedcellcompartments AT nicolastoro localizationofabacterialgroupiiintronencodedproteinineukaryoticnuclearsplicingrelatedcellcompartments
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Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.

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